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    PROPHAGE ELEMENT-FREE BACTERIA
    1.
    发明申请
    PROPHAGE ELEMENT-FREE BACTERIA 有权
    推荐元素免费细菌

    公开(公告)号：US20120219994A1

    公开(公告)日：2012-08-30

    申请号：US13363848

    申请日：2012-02-01

    申请人： FREDERICK R. BLATTNER ,  John W. Campbell ,  David Frisch ,  Guy Plunkett, III ,  Gyorgy Posfai

    发明人： FREDERICK R. BLATTNER ,  John W. Campbell ,  David Frisch ,  Guy Plunkett, III ,  Gyorgy Posfai

    IPC分类号： C12N1/20 ,  C12N1/21 ,  C12P21/02

    CPC分类号： C07K14/245 ,  C12N1/08 ,  C12N1/20 ,  C12N15/70 ,  C12P19/34 ,  C12P21/02 ,  C12R1/19

    摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

    摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。

    Prophage element-free bacteria
    2.
    发明授权
    Prophage element-free bacteria 有权
    无菌元素细菌

    公开(公告)号：US08765408B2

    公开(公告)日：2014-07-01

    申请号：US13363848

    申请日：2012-02-01

    申请人： Frederick R. Blattner ,  John W. Campbell ,  David Frisch ,  Guy Plunkett, III ,  Gyorgy Posfai

    发明人： Frederick R. Blattner ,  John W. Campbell ,  David Frisch ,  Guy Plunkett, III ,  Gyorgy Posfai

    IPC分类号： C12P21/06 ,  C12N1/20 ,  C12N1/21

    CPC分类号： C07K14/245 ,  C12N1/08 ,  C12N1/20 ,  C12N15/70 ,  C12P19/34 ,  C12P21/02 ,  C12R1/19

    摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

    摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株的基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。