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公开(公告)号：US08765408B2
公开(公告)日：2014-07-01
申请号：US13363848
申请日：2012-02-01
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株的基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US08119365B2
公开(公告)日：2012-02-21
申请号：US11400711
申请日：2006-04-07
摘要： A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.
摘要翻译： 提供了缺乏基因组和非基因组IS元件的细菌。 细菌可能更稳定并且可用于生产氨基酸，多肽，核酸和其它产物。
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公开(公告)号：US07303906B2
公开(公告)日：2007-12-04
申请号：US10655914
申请日：2003-09-05
申请人： Frederick R. Blattner , Gyorgy Posfai , Christopher D. Herring , Guy Plunkett, III , Jeremy Glasner , Trevor Twose
发明人： Frederick R. Blattner , Gyorgy Posfai , Christopher D. Herring , Guy Plunkett, III , Jeremy Glasner , Trevor Twose
CPC分类号： C12N1/08
摘要： The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specific E. coli strains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.
摘要翻译： 本发明公开了一种具有遗传工程化至少比其天然亲本菌株基因组小10％的基因组的细菌具有较好的转化能力。 公开了具有显着降低的基因组大小的特异性大肠杆菌菌株，其具有高度转化能力。 介绍了一种可以进一步提高转换效率的介质和方法。
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公开(公告)号：US06989265B2
公开(公告)日：2006-01-24
申请号：US10057582
申请日：2002-01-23
申请人： Frederick R. Blattner , Gyorgy Posfai , Christopher D. Herring , Guy Plunkett, III , Jeremy D. Glasner
发明人： Frederick R. Blattner , Gyorgy Posfai , Christopher D. Herring , Guy Plunkett, III , Jeremy D. Glasner
IPC分类号： C12N1/20
CPC分类号： C12N15/102 , C12N1/08 , C12R1/19
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
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公开(公告)号：US08178339B2
公开(公告)日：2012-05-15
申请号：US12064332
申请日：2006-08-18
摘要： Reduced genome strains of E. coli MG1655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
摘要翻译： 描述了大肠杆菌MG1655的减少的基因组菌株。 在各种实施方案中，与亲本菌株和市售菌株相比，菌株具有相同或改善的生长速率，转化效率，蛋白质表达，DNA产生，DNA产量和/或DNA质量的一种或多种。
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公开(公告)号：US20130011874A1
公开(公告)日：2013-01-10
申请号：US13445672
申请日：2012-04-12
摘要： Reduced genome strains of E. coli MG1 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
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公开(公告)号：US09085765B2
公开(公告)日：2015-07-21
申请号：US13445672
申请日：2012-04-12
摘要： Reduced genome strains of E. coli MGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
摘要翻译： 描述了大肠杆菌MG155的减少的基因组菌株。 在各种实施方案中，与亲本菌株和市售菌株相比，菌株具有相同或改善的生长速率，转化效率，蛋白质表达，DNA产生，DNA产量和/或DNA质量的一种或多种。
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公开(公告)号：US20090075333A1
公开(公告)日：2009-03-19
申请号：US12064332
申请日：2006-08-18
摘要： Reduced genome strains of E. coli MGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
摘要翻译： 描述了大肠杆菌MG155的减少的基因组菌株。 在各种实施方案中，与亲本菌株和市售菌株相比，菌株具有相同或改善的生长速率，转化效率，蛋白质表达，DNA产生，DNA产量和/或DNA质量的一种或多种。
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公开(公告)号：US08043842B2
公开(公告)日：2011-10-25
申请号：US11173900
申请日：2005-07-01
申请人： Frederick R. Blattner , Gyorgy Posfai , Christopher D. Herring , Guy Plunkett , Jeremy D. Glasner
发明人： Frederick R. Blattner , Gyorgy Posfai , Christopher D. Herring , Guy Plunkett , Jeremy D. Glasner
CPC分类号： C12N15/102 , C12N1/08 , C12R1/19
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化至少比其天然亲本菌株的基因组小至少2至14％的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US20100144548A1
公开(公告)日：2010-06-10
申请号：US12375911
申请日：2007-08-03
申请人： Waclaw Szybalski , Jadwiga Wild , Zdenka Hradecna , David Frisch , Frederick R. Blattner , Katarzyna Gromek
发明人： Waclaw Szybalski , Jadwiga Wild , Zdenka Hradecna , David Frisch , Frederick R. Blattner , Katarzyna Gromek
CPC分类号： C12N15/70 , C12N15/1093 , C12N15/64 , C12N15/66
摘要： The present invention relates generally to the field of molecular biology and genomics. More specifically, the present invention concerns the cloning of nucleic acid molecules and the production of nucleic acid libraries, as well as the expression of recombinant proteins and bactofection.
摘要翻译： 本发明一般涉及分子生物学和基因组学领域。 更具体地，本发明涉及核酸分子的克隆和核酸文库的产生，以及重组蛋白质和细菌转染的表达。
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