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    Substrate probe for use in measuring enzyme activity
    1.
    发明授权
    Substrate probe for use in measuring enzyme activity 有权
    用于测量酶活性的底物探针

    公开(公告)号：US08481254B2

    公开(公告)日：2013-07-09

    申请号：US12675511

    申请日：2008-09-08

    申请人： Fumio Yamauchi ,  Kimihiro Yoshimura ,  Katsuaki Kuge ,  Tetsuya Yano ,  Masahiro Shirakawa ,  Yasuhiro Aoyama ,  Shinsuke Sando

    发明人： Fumio Yamauchi ,  Kimihiro Yoshimura ,  Katsuaki Kuge ,  Tetsuya Yano ,  Masahiro Shirakawa ,  Yasuhiro Aoyama ,  Shinsuke Sando

    IPC分类号： C12Q1/00

    CPC分类号： C12Q1/54 ,  C12Q1/32 ,  C12Q1/34 ,  G01N24/088 ,  G01R33/281 ,  G01R33/4633 ,  G01R33/465 ,  G01R33/483 ,  G01R33/5601 ,  Y02P20/582

    摘要： Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.

    摘要翻译： 能够高精度地检测酶活性的底物探针和使用底物探针的多核磁共振法检测酶活性的方法。 通过使用用于通过多维核磁共振法测定酶活性的底物探针进行多维核磁共振，其特征在于含有被活性酶选择性识别的酶识别位点作为 至少一个结构单元和至少三个具有核自旋和不同共振频率的核磁共振活性核连接的基团，其特异性地存在于酶识别中，从而检测底物探针和酶的存在 活动。 或者，通过多维核共振成像方法进行酶活性的成像。

    Molecules capable of binding to telomere and the like and method with the use of the same
    4.
    发明授权
    Molecules capable of binding to telomere and the like and method with the use of the same 失效
    能够结合端粒等的分子及其使用方法

    公开(公告)号：US07351531B2

    公开(公告)日：2008-04-01

    申请号：US10333152

    申请日：2001-07-17

    申请人： Isao Saito ,  Kazuhiko Nakatani ,  Shinsuke Sando

    发明人： Isao Saito ,  Kazuhiko Nakatani ,  Shinsuke Sando

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02

    CPC分类号： C07D519/00 ,  A61K31/4375

    摘要： A method of forming a self-connective structure (a hairpin structure, a four-stranded structure, etc.) of a single-stranded oligonucleotide chain by forming a mimetic base pair of a mismatch base pair failing to form any normal base pair among base pairs of the single-stranded oligonucleotide chain with the use of a compounds represented by the following general formula (I): A-L-B (I) wherein A represents a chemical structural moiety capable of forming a pair with one base of a base pair failing to form any normal base pair; B represents a chemical structural moiety capable of forming a pair with the other base of the base pair failing to form any normal base pair; and L represents a linker structure by which the chemical structures A and B are linked to each other; and a method of inhibiting the activity of enzyme according to the previous method.

    摘要翻译： 通过形成不能在碱基中形成任何正常碱基对的错配碱基对的模拟碱基对，形成单链寡核苷酸链的自连接结构（发夹结构，四链结构等）的方法 使用由以下通式（I）表示的化合物的单链寡核苷酸链对：ALB（I）其中A表示能够与未成形的碱基对的一个碱基形成一对的化学结构部分 任何正常的碱基对; B表示能够与碱基对的另一个碱基形成一对而不能形成任何正常碱基对的化学结构部分; L表示化学结构A和B彼此连接的连接体结构; 以及根据前述方法抑制酶的活性的方法。

    Mismatch-recognizing molecules
    5.
    发明授权
    Mismatch-recognizing molecules 失效
    不匹配识别分子

    公开(公告)号：US06607890B1

    公开(公告)日：2003-08-19

    申请号：US09889976

    申请日：2001-07-24

    申请人： Kazuhiko Nakatani ,  Isao Saito ,  Shinsuke Sando

    发明人： Kazuhiko Nakatani ,  Isao Saito ,  Shinsuke Sando

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6832 ,  C12Q2527/125 ,  C12Q2527/107

    摘要： A method whereby a mismatched base pair in a nucleic acid such as DNA or RNA can be conveniently and highly sensitively detected; and detection reagents therefor. A method comprising, in a base pair failing to form a normal base pair, forming a mimetic base pair with the use of a mismatch-recognizing molecule represented by the following general formula (I): A—L—B (I) wherein A represents a chemical structure moiety capable of forming a pair with one of the bases of the base pair failing to form a normal base pair; B represents a chemical structure moiety capable of forming a pair with the other of the bases of the base pair failing to form a normal base pair; and L represents a linker structure whereby the chemical structure moieties A and B are linked to each other; and measuring the formation of the above-described mimetic base pair to thereby detect and identify the base pair failing to form a normal base pair; kits therefor; reagents for detecting a mismatch; and a method of detecting an abnormality in the base sequence of a gene.

    摘要翻译： 可以方便，高度敏感地检测核酸如DNA或RNA中的错配碱基对的方法; 及其检测试剂。 在不能形成正常碱基对的碱基对中，使用由以下通式（I）表示的错配识别分子形成模拟碱基对：ALB（I）其中A表示化学结构 能够与碱基对的碱基之一形成一对的部分未能形成正常的碱基对; B表示能够与碱基对的另一个碱基形成正常碱基对的化学结构部分; L表示连接结构，其中化学结构部分A和B彼此连接; 并测量上述模拟碱基对的形成，从而检测和鉴定不能形成正常碱基对的碱基对; 套装; 用于检测错配的试剂; 以及检测基因的碱基序列异常的方法。