公开(公告)号：US20180119084A1

公开(公告)日：2018-05-03

申请号：US15561826

申请日：2016-04-12

Applicant: GE HEALTHCARE BIO-SCIENCES CORP.

Inventor： Colin R. Tuohey ,  Richard Lee Damren ,  Thomas John Erdenberger ,  Parish M. Galliher ,  Patrick M. Guertin

IPC: C12M1/12 ,  C12N7/00 ,  C12M1/00 ,  C12M1/02 ,  A61L2/00 ,  C12M1/34

CPC classification number: C12M37/00 ,  A61L2/0017 ,  A61L2/0023 ,  A61L2/0035 ,  A61L2/081 ,  A61L2202/14 ,  A61L2202/23 ,  C12M23/06 ,  C12M23/14 ,  C12M23/26 ,  C12M23/28 ,  C12M27/02 ,  C12M29/00 ,  C12M41/16 ,  C12M41/22 ,  C12N7/00 ,  C12N2795/00061

Abstract: The invention discloses a bioreactor apparatus (1;101;201;301) for cultivation of cells comprising: a) a disposable bioreactor vessel (2) with one or more walls (3,4,5) defining an inner volume (6), at least one port (10) in a wall, wherein the disposable bioreactor vessel is positioned in a rigid support structure (8;108); and b) a heater (9;109;209;309), capable of heating an amount of culture medium to a target temperature in the range of 55-95° C., while the amount of culture medium is being confined in or conveyed to the inner volume.

公开(公告)号：US09655960B2

公开(公告)日：2017-05-23

申请号：US14267942

申请日：2014-05-02

Applicant: GE HEALTHCARE BIO-SCIENCES CORP.

Inventor： Cynthia K. Lee ,  Thomas P. Monath ,  Patrick M. Guertin ,  Edward G. Hayman

IPC: A61K39/12 ,  C12N7/01 ,  C12N7/00 ,  A61K39/00

CPC classification number: A61K39/12 ,  A61K2039/5252 ,  A61K2039/55505 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2770/24121 ,  C12N2770/24134 ,  Y02A50/388

Abstract: The invention provides a an inactive, non-replicating vaccine comprising whole virion, chemically inactivated Yellow Fever virus which is inactivated using a method that ensures preservation of critical, neutralizing epitopes. The Yellow Fever virus has been adapted to propagate in cells to higher yields than the unadapted virus. The invention also provides methods for preventing Yellow Fever viral infection.

公开(公告)号：US09932562B2

公开(公告)日：2018-04-03

申请号：US14054461

申请日：2013-10-15

Applicant: GE Healthcare Bio-Sciences Corp.

Inventor： Patrick M. Guertin ,  Joseph D. Crowell

IPC: C12N7/00 ,  C12M1/12 ,  C12M1/00

CPC classification number: C12N7/00 ,  C12M25/14 ,  C12M47/10 ,  C12N2770/24151

Abstract: The invention provides a method of increasing product yield per culture in a population of product-secreting cells bound to a scaffold, for example, a microcarrier, at least partially immersed in a culture medium in a bioreactor. The method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration while leaving the scaffold with the bound cells in the bioreactor; re-feeding the cells by adding a fresh culture medium to increase the culture medium in the bioreactor to approximately the original volume; agitating the culture medium to allow the cells to grow and release a second-secreted product concentration; harvesting product by removing the culture medium with the second-secreted product concentration while leaving the scaffold with the bound cells behind. Also disclosed is a method of increasing virus yield per culture in a population of virus-infected cells bound to a microcarrier suspended in a culture medium.