公开(公告)号：US10751645B2

公开(公告)日：2020-08-25

申请号：US15882998

申请日：2018-01-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J Johansson ,  Jean-Luc Maloisel

IPC: B01D15/38 ,  B01D15/32 ,  B01D15/36 ,  B01J20/32 ,  B01J20/286 ,  B01J41/20 ,  C07K1/36 ,  C07K16/06 ,  C07K1/16 ,  C07K16/00

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.