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公开(公告)号：US10343142B2
公开(公告)日：2019-07-09
申请号：US16189894
申请日：2018-11-13
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC: B01J20/24 , B01D15/38 , B01J20/286 , C07K1/22 , B01J20/32 , C07K14/31 , C07K16/00 , C07K17/00 , B01J20/28 , B01J20/285 , B01J20/289 , C07K17/10
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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Patent Agency Ranking
公开(公告)号：US09663559B2
公开(公告)日：2017-05-30
申请号：US15063471
申请日：2016-03-07
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
CPC classification number: B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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3.发明申请公开(公告)号：US20190060869A1
公开(公告)日：2019-02-28
申请号：US16189894
申请日：2018-11-13
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC: B01J20/24 , B01D15/38 , C07K16/00 , B01J20/32 , C07K17/10 , B01J20/28 , B01J20/285 , B01J20/289 , C07K14/31 , C07K1/22 , B01J20/286 , C07K17/00
CPC classification number: B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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公开(公告)号：US10213765B2
公开(公告)日：2019-02-26
申请号：US15603285
申请日：2017-05-23
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC: C07K1/22 , B01D15/38 , B01J20/24 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K17/00 , B01J20/28 , B01J20/285 , B01J20/289 , C07K17/10
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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公开(公告)号：US10189891B2
公开(公告)日：2019-01-29
申请号：US14385336
申请日：2013-03-26
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Mats Ander , Goran Bauren , Tomas Bjorkman , Per-Mikael Aberg , Gustav Rodrigo
IPC: C07K1/22 , C07K14/31 , C07K16/00 , B01J20/286 , B01D15/38 , B01J20/291 , G01N33/68 , B01J20/32
Abstract: The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine.
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6.发明申请公开(公告)号：US20170259244A1
公开(公告)日：2017-09-14
申请号：US15603285
申请日：2017-05-23
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC: B01J20/24 , C07K14/31 , C07K17/10 , B01D15/38 , B01J20/28 , B01J20/285 , B01J20/289 , B01J20/32 , C07K1/22 , C07K16/00
CPC classification number: B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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