公开(公告)号：US11035012B2

公开(公告)日：2021-06-15

申请号：US15904232

申请日：2018-02-23

Applicant: GEN-PROBE INCORPORATED

Inventor： Reinhold Pollner ,  Mehrdad Majlessi ,  Susan Yamagata ,  Michael M. Becker ,  Mark Reynolds ,  Lyle Arnold

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/70 ,  C12Q1/6837

Abstract: The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.

公开(公告)号：US20180251862A1

公开(公告)日：2018-09-06

申请号：US15904232

申请日：2018-02-23

Applicant: GEN-PROBE INCORPORATED

Inventor： Reinhold Pollner ,  Mehrdad Majlessi ,  Susan Yamagata ,  Michael M. Becker ,  Mark Reynolds ,  Lyle Arnold

IPC: C12Q1/70 ,  C12Q1/6837

CPC classification number: C12Q1/707 ,  C12Q1/6837 ,  C12Q1/703 ,  Y02A50/53 ,  Y02A50/54 ,  C12Q2525/161

Abstract: The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.