公开(公告)号：US09915592B2

公开(公告)日：2018-03-13

申请号：US14772305

申请日：2014-03-05

Applicant: General Electric Company

Inventor： Anup Sood ,  Kevin Bernard Kenny ,  Arunkumar Natarajan ,  Lakshmi Sireesha Kaanumalle ,  Elizabeth Mary McDonough

IPC: G01N1/30 ,  G01N33/50 ,  G01N33/58

CPC classification number: G01N1/30 ,  G01N33/5005 ,  G01N33/582

Abstract: Methods comprising probing multiple targets in an H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, optionally detecting H&E staining of the sample, removing the hematoxylin and eosin signals, and detecting additional features or targets in the biological sample. The detecting step may include performing the steps of binding at least one probe to one or more targets to the sample, detecting a signal from the probe and contacting the sample with a bleaching agent. The process of binding, detecting and bleaching may be iteratively repeated.

公开(公告)号：US20170122872A1

公开(公告)日：2017-05-04

申请号：US15299085

申请日：2016-10-20

Applicant: General Electric Company

Inventor： Anup Sood ,  ARUNKUMAR NATARAJAN ,  Dmitry Dylov ,  Lakshmi Sireesha Kaanumalle ,  Elizabeth Mary McDonough

IPC: G01N21/64 ,  G01N33/574 ,  G01N33/483

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N33/4833 ,  G01N33/57407 ,  G01N2021/6439 ,  G01N2333/70503 ,  G01N2333/91215

Abstract: The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of: a) providing a biological microscopy sample; b) irradiating the sample with visible light, wherein the visible light has a spectrum such that at least 50% of the light intensity emanates from a narrow wavelength interval within the visible range. The invention also discloses a method for autofluorescence reduction with triplet sensitizers irradiated with visible light.

公开(公告)号：US20160069782A1

公开(公告)日：2016-03-10

申请号：US14772305

申请日：2014-03-05

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Anup Sood ,  Kevin Bernard Kenny ,  Arunkumar Natarajan ,  Lakshmi Sireesha Kaanumalle ,  Elizabeth Mary McDonough

IPC: G01N1/30 ,  G01N33/58

CPC classification number: G01N1/30 ,  G01N33/5005 ,  G01N33/582

Abstract: Methods comprising probing multiple targets in an H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, optionally detecting H&E staining of the sample, removing the hematoxylin and eosin signals, and detecting additional features or targets in the biological sample. The detecting step may include performing the steps of binding at least one probe to one or more targets to the sample, detecting a signal from the probe and contacting the sample with a bleaching agent. The process of binding, detecting and bleaching may be iteratively repeated.

Abstract translation: 提供了包括在H＆E染色的生物样品中探测多个靶标的方法。 所述方法包括提供一个苏木精和曙红含有多个目标染色生物样本，任选检测所述样品的H＆E染色，除去苏木精和曙红信号，并且检测生物样品中另外的特征或对象的步骤。 检测步骤可以包括执行中的至少一个探针结合到一个或多个目标的样品中，检测来自探针的信号，并且与漂白剂接触所述样品的步骤。 结合，检测和漂白的过程可以迭代重复。