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公开(公告)号：US20240279828A1
公开(公告)日：2024-08-22
申请号：US18565339
申请日：2022-05-31
发明人： Feichi HU , Michael W. REED , John COOPER , Jackson KELLOCK , Cheng-Hsien WU
IPC分类号： C25B11/085 , B01J19/00 , C07C245/20 , C25B3/07 , C25B3/09 , C25B3/29 , C25B11/052 , C25B11/061 , C25B11/095 , C25D9/02 , C25F1/00
CPC分类号： C25B11/085 , B01J19/0046 , C07C245/20 , C25B3/07 , C25B3/09 , C25B3/29 , C25B11/052 , C25B11/061 , C25B11/095 , C25D9/02 , C25F1/00 , B01J2219/00596 , B01J2219/00713 , B01J2219/00722
摘要： Functionalized aryldiazonium salts and films formed by electrografting of functionalized aryldiazonium salts are provided. Methods for purifying functionalized aryldiazonium salts and for coating solid support systems with functionalized aryldiazonium salts are also provided. These coated solid support systems can be used, for example, in methods of oligonucleotide synthesis.
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公开(公告)号：US20240200126A1
公开(公告)日：2024-06-20
申请号：US18287245
申请日：2022-04-20
发明人： Feichi HU , Qi WANG , Cheng-Hsien WU , Nileshi SARAF
IPC分类号： C12Q1/6858 , C12Q1/6876
CPC分类号： C12Q1/6858 , C12Q1/6876
摘要： A primer group and method for detecting single-base mutations. The primer group comprises the following primers: an identification primer, which is composed of, from the 5′ end to the 3′ end, (a) a nucleotide sequence which complements a segment of continuous nucleotides in a nucleic acid sequence to be detected, wherein the 5′ end of the continuous nucleotides starts at a first nucleotide downstream of an expected mutation site; and (b) a nucleotide which complements a non-mutated nucleotide or an expected mutated nucleotide at the expected single-base mutation site of the nucleic acid sequence. The primer group also comprises an amplification primer. The amplification primer is capable of using the identification primer to amplify an amplification product which is obtained by amplifying the nucleic acid sequence. The identification primer is 1 to 19 nucleotides less than the amplification primer.
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