公开(公告)号：US20160108094A1

公开(公告)日：2016-04-21

申请号：US14927052

申请日：2015-10-29

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Jeannette ADU-BOBIE ,  Mariagrazia PIZZA ,  Nathalie NORAIS ,  Germano FERRARI ,  Guido GRANDI

IPC: C07K14/245 ,  C07K14/22

CPC classification number: C07K14/245 ,  A61K39/00 ,  A61K39/0258 ,  A61K39/095 ,  C07K14/22 ,  C12N9/1051 ,  Y02A50/474

Abstract: Knockout of the meningococcal mltA homolog gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titres than OMVs prepared by normal production processes. Thus the invention provides a bacterium having a knockout mutation of its mltA gene. The invention also provides a bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan; and (ii) does not express a protein having the lytic transglycosylase activity MltA protein. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.

Abstract translation: 脑膜炎球菌mltA同系物的敲除使细菌自发释放富含免疫原性外膜蛋白的囊泡，并且能够以比正常生产过程制备的OMV更高的杀菌滴度引发交叉保护性抗体应答。 因此，本发明提供了具有其mltA基因的敲除突变的细菌。 本发明还提供了一种细菌，其中所述细菌：（i）具有包含肽聚糖的细胞壁; 和（ii）不表达具有溶解性转糖苷酶活性MltA蛋白质的蛋白质。 本发明还提供包含囊泡的组合物，其在本发明的细菌培养期间释放到培养基中。

公开(公告)号：US20180009852A1

公开(公告)日：2018-01-11

申请号：US15698434

申请日：2017-09-07

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Jeannette ADU-BOBIE ,  Mariagrazia PIZZA ,  Nathalie NORAIS ,  Germano FERRARI ,  Guido Grandi

IPC: C07K14/245 ,  A61K39/095 ,  C12N9/10 ,  C07K14/22 ,  A61K39/00 ,  A61K39/108

CPC classification number: C07K14/245 ,  A61K39/00 ,  A61K39/0258 ,  A61K39/095 ,  C07K14/22 ,  C12N9/1051 ,  Y02A50/474

Abstract: Knockout of the meningococcal mltA homolog gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titres than OMVs prepared by normal production processes. Thus the invention provides a bacterium having a knockout mutation of its mltA gene. The invention also provides a bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan; and (ii) does not express a protein having the lytic transglycosylase activity MltA protein. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.