公开(公告)号：US20230193335A1

公开(公告)日：2023-06-22

申请号：US17759270

申请日：2021-01-22

Applicant: GLYCOM A/S

Inventor： Margit PEDERSEN ,  Manos PAPADAKIS ,  Katrine Bych KAMPMANN

IPC: C12P19/04 ,  C12N9/10 ,  C12N15/70 ,  C07K14/24

CPC classification number: C12P19/04 ,  C12N9/1051 ,  C12Y204/01065 ,  C12Y204/01146 ,  C12N15/70 ,  C07K14/24

Abstract: The present inventive concept relates to a genetically modified cell enabled for the production of an oligosaccharide, preferably, an HMO, comprising a recombinant nucleic encoding a putative transporter protein of the MFS superfamily; and methods using said cell for the production of oligosaccharide(s), preferably an HMO.

公开(公告)号：US20240102063A1

公开(公告)日：2024-03-28

申请号：US18262099

申请日：2022-01-21

Applicant: GLYCOM A/S

Inventor： Margit PEDERSEN ,  Isotta D'ARRIGO ,  Katrine BYCH KAMPMANN ,  Manos PAPADAKIS

IPC: C12P19/04 ,  C07K14/24 ,  C12N9/10 ,  C12N15/52 ,  C12P19/18

CPC classification number: C12P19/04 ,  C07K14/24 ,  C12N9/1081 ,  C12N15/52 ,  C12P19/18 ,  C12Y204/99001 ,  C12Y204/99004

Abstract: The present invention relates to the field of recombinant production of biological molecules in genetically modified cells. More particularly, it relates to a method for recombinant production of sialylated human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS), the protein expressed being Fred.

公开(公告)号：US20230109937A1

公开(公告)日：2023-04-13

申请号：US17759276

申请日：2021-01-22

Applicant: GLYCOM A/S

Inventor： Margit PEDERSEN ,  Isotta D'ARRIGO ,  Katrine Bych KAMPMANN ,  Manos PAPADAKIS

IPC: C12N15/70 ,  C12P19/00 ,  C07H3/06 ,  C12N1/20

Abstract: The present invention relates to the field of recombinant production of biological molecules in genetically modified cells. More particularly, it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS), the protein expressed being Fred.

公开(公告)号：US20180371001A1

公开(公告)日：2018-12-27

申请号：US16063465

申请日：2016-12-19

Applicant: GLYCOM A/S

Inventor： Manos PAPADAKIS ,  Margit PEDERSEN ,  Katrine BYCH

IPC: C07H3/06 ,  C07H1/00 ,  C12N15/70 ,  C07H3/02 ,  C12P19/18

CPC classification number: C07H3/06 ,  C07H1/00 ,  C07H3/02 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12N15/70 ,  C12P19/18 ,  C12Y204/99001

Abstract: The invention relates to a microbial production of recombinant oligosaccharides, particularly of human milk oligosaccharides (HMOs), using a genetically modified microorganism, particularly E. coli, in antibiotic-free cultivating medium.

公开(公告)号：US20230072639A1

公开(公告)日：2023-03-09

申请号：US17759267

申请日：2021-01-22

Applicant: GLYCOM A/S

Inventor： Margit PEDERSEN ,  Isotta D'ARRIGO ,  Katrine Bych KAMPMANN ,  Manos PAPADAKIS

IPC: C12N5/10 ,  C12P19/00 ,  C12N15/74

Abstract: The present invention relates to the field of recombinant production of biological molecules in genetically modified cells. More particularly, it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS), the protein expressed being Bad.

公开(公告)号：US20220282262A1

公开(公告)日：2022-09-08

申请号：US17299583

申请日：2019-12-04

Applicant: GLYCOM A/S

Inventor： Manos PAPADAKIS ,  Margit PEDERSEN

IPC: C12N15/70 ,  C12N9/10 ,  C12P19/18

Abstract: The present invention provides a method for biotechnological production of LNFP-V by using recombinant bacterial cells. LNFP-V is produced by using a polypeptide having an a1,3/4-fucosyl transferase activity and comprising or consisting of an amino acid sequence that has a sequence identity of at least 90% with amino acid sequence of SEQ ID No. 1 or 3.

公开(公告)号：US20210102216A1

公开(公告)日：2021-04-08

申请号：US16956092

申请日：2018-12-19

Applicant: GLYCOM A/S

Inventor： Margit PEDERSEN ,  Manos PAPADAKIS

IPC: C12N15/70

Abstract: The present invention relates to the field of recombining production of biological molecules in host cells. The invention provides nucleic acid constructs that allow to modify expression of a desired gene using both in vitro and in vivo gene expression systems. The constructs can advantageously be used to produce a variety of biological molecules recombinantly in industrial scales, e.g. human milk oligosaccharides (HMO).