公开(公告)号：US4959303A

公开(公告)日：1990-09-25

申请号：US135869

申请日：1987-12-21

申请人： Gary L. Milburn ,  Judith Rabbie ,  Thomas M. Houts

发明人： Gary L. Milburn ,  Judith Rabbie ,  Thomas M. Houts

IPC分类号： G01N33/543 ,  G01N33/569 ,  G01N33/571

CPC分类号： G01N33/54306 ,  G01N33/56911 ,  G01N33/571 ,  Y10S435/871 ,  Y10S436/824 ,  Y10S436/828

摘要： Methods are disclosed for detecting an antigen in a biological sample. The methods involve providing in combination a solid support, which is substantially free of specific binding proteins, and a medium comprising an antigen from the sample and an antibody for the antigen. The combination is incubated under conditions sufficient for the antibody when bound to the antigen to bind to the support. The presence or amount of antibody on the support or in the medium is determined and is related to the presence of antigen in the sample. The methods have particular application to the detection of gram-negative bacteria.

公开(公告)号：US5187066A

公开(公告)日：1993-02-16

申请号：US479930

申请日：1990-02-14

申请人： Martin Becker ,  Nurith Kurn ,  Yen P. Liu ,  Rajesh D. Patel ,  Thomas M. Houts ,  John D. Olson

发明人： Martin Becker ,  Nurith Kurn ,  Yen P. Liu ,  Rajesh D. Patel ,  Thomas M. Houts ,  John D. Olson

IPC分类号： G01N33/543 ,  G01N33/569 ,  G01N33/571

CPC分类号： G01N33/543 ,  G01N33/56927 ,  G01N33/571

摘要： This invention is to a method for detecting an amphiphilic antigen in a biological sample suspected of containing the amphiphilic antigen, which method comprises providing in combination a hydrophilic solid support modified to have a hydrophobic surface and an assay medium suspected of containing an amphiphilic antigen, incubating the combination under conditions sufficient for the amphiphilic antigen to bind to the hydrophobic surface, and determining the presence or amount of the amphiphilic antigen bound to the hydrophobic surface.

摘要翻译： 本发明涉及一种用于检测怀疑含有两亲性抗原的生物样品中的两亲性抗原的方法，该方法包括组合改性以具有疏水表面的亲水性固体支持物和怀疑含有两亲性抗原的测定培养基 所述组合在足以使两亲性抗原结合到疏水性表面的条件下，以及确定与疏水性表面结合的两亲性抗原的存在或量。

公开(公告)号：US4465775A

公开(公告)日：1984-08-14

申请号：US381267

申请日：1982-05-24

申请人： Thomas M. Houts

发明人： Thomas M. Houts

IPC分类号： G01N33/53 ,  C07H23/00 ,  G01N33/532 ,  G01N33/82 ,  G01N33/60 ,  C08G77/04 ,  G01N33/50

CPC分类号： C07H23/00 ,  G01N33/82

摘要： Vitamin B.sub.12 in liquid samples, such as human serum or human plasma, is assayed by a competitive binding technique using intrinsic factor and certain labelled vitamin B.sub.12 derivatives. The labelled derivatives are formed from the (d)-monocarboxylic acid isomer of vitamin B.sub.12, which isomer is free from other monocarboxylic acid isomers (and derivatives thereof) of vitamin B.sub.12, by binding to the (d)-isomer, via the carboxylic group, a compound which is itself a label (e.g. an enzyme) or which comprises a label (e.g. a fluorophore), or to which a label is attached (e.g. a histidine ester to which .sup.125 I is attached). The labelled derivatives of the (d)-monocarboxylic acid of vitamin B.sub.12, free from other isomeric monocarboxylic acids and derivatives, are novel and constitute one aspect of the invention.

摘要翻译： 通过使用内在因子和某些标记的维生素B12衍生物的竞争性结合技术测定液体样品（例如人血清或人血浆）中的维生素B12。 标记的衍生物由维生素B12的（d） - 单羧酸异构体形成，该维生素B12通过经由羧基与（D） - 异构体结合而不含维生素B12的其它单羧酸异构体（及其衍生物） ，本身是标记（例如酶）或包含标记（例如荧光团）或附着标签的组合物（例如附着125I的组氨酸酯）的化合物。 不含其它异构单羧酸及其衍生物的维生素B12（d） - 单羧酸的标记衍生物是新颖的，构成本发明的一个方面。

公开(公告)号：US4883688A

公开(公告)日：1989-11-28

申请号：US215020

申请日：1988-07-05

申请人： Thomas M. Houts ,  Edwin F. Ullman

发明人： Thomas M. Houts ,  Edwin F. Ullman

IPC分类号： G01N30/90 ,  G01N30/92

CPC分类号： G01N30/92 ,  G01N30/90 ,  Y10T83/0558

摘要： A device and a method for producing the device for use in a chromatographic system wherein a component of a mixture is partitioned is comprised of a chromatographic material. In the chromatographic system the component traverses at least a portion of the chromatographic material. The device generally has at least one longitudinal edge substantially corresponding to the direction of traverse of the component. The longitudinal edge is modified to control the shape of the fron t of the traversing component by introducing along the edge either a plurality of indentations or a chemical substance.

摘要翻译： 用于生产用于色谱系统的装置的装置和方法，其中混合物的组分被分离由色谱材料组成。 在色谱系统中，组分穿过至少一部分色谱材料。 该装置通常具有基本上对应于部件的横向方向的至少一个纵向边缘。 纵向边缘被修改以通过沿边缘引入多个凹口或化学物质来控制横向部件的形状。

公开(公告)号：US4757004A

公开(公告)日：1988-07-12

申请号：US591155

申请日：1984-03-16

申请人： Thomas M. Houts ,  Edwin F. Ullman

发明人： Thomas M. Houts ,  Edwin F. Ullman

IPC分类号： B01D15/08 ,  G01N30/60 ,  G01N30/90 ,  G01N30/92 ,  G01N33/543 ,  G01N33/544 ,  G01N33/53

CPC分类号： G01N30/90 ,  G01N30/92 ,  Y10S435/803 ,  Y10S435/805 ,  Y10S436/81

摘要： A device is disclosed for use in a chromatographic system wherein a component of a mixture is paritioned between a liquid phase and an immobile phase. The device is comprised of a chromatographic material. In the chromatographic system the component traverses at least a portion of the chromatographic material. The device generally has at least one longitudinal edge substantially corresponding to the direction of traverse of the component. The longitudinal edge includes means for controlling the shape of the front of the traversing component.

摘要翻译： 公开了用于色谱系统的装置，其中混合物的组分在液相和固定相之间分配。 该装置由色谱材料组成。 在色谱系统中，组分穿过至少一部分色谱材料。 该装置通常具有基本上对应于部件的横向方向的至少一个纵向边缘。 纵向边缘包括用于控制横向部件前部的形状的装置。