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    Particle-Based Analyte Characterization
    4.
    发明申请
    Particle-Based Analyte Characterization 审中-公开
    基于粒子的分析物表征

    公开(公告)号：US20090220989A1

    公开(公告)日：2009-09-03

    申请号：US12096342

    申请日：2006-12-05

    申请人： Kamala Tyagarajan ,  Dianne M. Fishwild ,  David A. King

    发明人： Kamala Tyagarajan ,  Dianne M. Fishwild ,  David A. King

    IPC分类号： G01N33/53 ,  C12M1/34

    CPC分类号： G01N33/54346 ,  G01N33/582

    摘要： Methods for assaying a sample for an analyte are provided. In various embodiments, the methods comprise contacting a sample suspected of containing the analyte with a non-uniform particle comprising a capture molecule, and further contacting the particle with a detection moiety comprising a label that permits detection of the analyte when associated with the particle. The methods may be performed to detect and/or quantitate analyte in the sample. In some embodiments, the methods may be performed in an automated manner, and may use an optical and/or cytometric apparatus for performing the method(s). The methods may further be performed with automated vessel-processing apparatus(es), such as plate loaders, plate washers, etc. Also provided are complexes containing the described materials formed by an assay of the invention, including excited state complexes. Kits useful for performing such methods are also provided.

    摘要翻译： 提供了用于分析样品的分析物的方法。 在各种实施方案中，所述方法包括将怀疑含有分析物的样品与包含捕获分子的非均匀颗粒接触，并且使颗粒与包含标记的检测部分接触，所述标记允许在与颗粒相关联时检测分析物。 可以执行该方法以检测和/或定量样品中的分析物。 在一些实施例中，可以以自动方式执行所述方法，并且可以使用用于执行所述方法的光学和/或细胞计数装置。 这些方法可以进一步用自动容器处理设备（例如平板装载机，板式洗涤器等）进行。还提供了含有通过本发明的测定形成的所述材料的复合物，包括激发态复合物。 还提供了用于执行这些方法的套件。