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    Electrophoretic nucleic acid purification method
    2.
    发明授权
    Electrophoretic nucleic acid purification method 失效
    电泳核酸纯化方法

    公开(公告)号：US6146511A

    公开(公告)日：2000-11-14

    申请号：US016531

    申请日：1998-01-30

    申请人： Gary Slater ,  J. William Efcavitch ,  Guy Drouin ,  Pascal Mayer ,  Jean Rousseau ,  Hong Yan Zhou ,  Claudia Chiesa ,  Robert Ruhfel ,  Roger O'Neill

    发明人： Gary Slater ,  J. William Efcavitch ,  Guy Drouin ,  Pascal Mayer ,  Jean Rousseau ,  Hong Yan Zhou ,  Claudia Chiesa ,  Robert Ruhfel ,  Roger O'Neill

    IPC分类号： B01D57/02 ,  B03C5/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/09 ,  C12N15/10 ,  G01N27/447 ,  G01N27/26

    CPC分类号： C12N15/101 ,  G01N27/447

    摘要： An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well. According to the method, the first and second electrophoresis steps are effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating. Also disclosed is a preparative electrophoresis method employing an alternating electrical field.

    摘要翻译： 公开了一种用于纯化核酸样品的电泳方法。 该方法通常包括以下步骤：（1）提供包含所需核酸和一种或多种污染物的核酸样品，（2）提供具有载体孔的电泳基质和其中形成的回收孔，（3） （4）进行第一次电泳，其中包括使核酸样品首次有效地将所需核酸转运到载体孔中并进入电泳基质中; 包括进行第二次电泳，其包括将核酸样品电泳第二次，有效将所需核酸转运到电泳基质内并进入回收井。 根据该方法，第一和第二电泳步骤对于相对于核酸样品中所需核酸浓度显着降低污染物的浓度是有效的，从而产生纯化的核酸。 在该方法中，加载和回收井可以相同或不同，并且电场可以是直流或交替的。 还公开了采用交流电场的制备电泳方法。