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1.发明授权Methods for quantitative amplification and detection over a wide dynamic range 有权在宽动态范围内进行定量扩增和检测的方法公开(公告)号：US08932817B2
公开(公告)日：2015-01-13
申请号：US14064427
申请日：2013-10-28
Applicant: Gen-Probe Incorporated
Inventor： Daniel L. Kacian , Kenneth A. Browne
CPC classification number: C12Q1/6851 , C12Q1/6813 , C12Q1/6865 , C12Q2545/101 , C12Q2537/143
Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
Abstract translation: 公开了使用单个扩增系统在单个容器中以不等比例制备可微分扩增子的组合物和方法。 选择可差分扩增子的数目及其彼此的比例以跨越扩增反应的所需线性动态范围，并适应用于确定产生的扩增子的量的测量系统的限制。 通过提供不等量的一个或多个扩增反应组分（例如，可区分的扩增寡核苷酸，NTP混合物中的天然和非天然NTP等）产生不等量的可区分的扩增子种类。 使用从检测动态范围内的一种或多种扩增子种检测产生的线性检测范围来确定测试样品中存在的靶核酸的量。
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公开(公告)号：USRE45194E1
公开(公告)日：2014-10-14
申请号：US14076031
申请日：2013-11-08
Applicant: Gen-Probe Incorporated
Inventor： Daniel L. Kacian , Mark R. Kennedy , Nick M. Carter
CPC classification number: B01L3/565 , B01L3/50825 , B01L2200/026 , B01L2300/044 , B01L2300/047 , B01L2300/0681 , B01L2300/069 , B01L2300/0887 , B65D41/0414 , B65D51/002 , B65D51/1616 , B65G2203/044 , H01L21/67784
Abstract: A cap having a core structure dimensioned to receive a pipette therethrough. The cap includes two axially aligned frangible seals that are affixed to the core structure in a spaced-apart relationship. The frangible seals are constructed so that air passageways are formed between the frangible seals and a pipette tip when the pipette tip penetrates the frangible seals. The cap optionally includes a filter interposed between the first and second frangible seals.
Abstract translation: 具有芯体结构的帽，其尺寸适于接收通过其的移液管。 盖子包括两个轴向对齐的易碎密封件，其以间隔的关系固定到芯部结构。 脆弱的密封件被构造成使得当移液管尖端穿透易碎密封件时，易碎密封件和移液管尖端之间形成空气通道。 盖可选地包括插入在第一和第二易碎密封件之间的过滤器。
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Patent Agency Ranking
公开(公告)号：US09856527B2
公开(公告)日：2018-01-02
申请号：US15162263
申请日：2016-05-23
Applicant: GEN-PROBE INCORPORATED
Inventor： Daniel L. Kacian , Kenneth A. Browne
CPC classification number: C12Q1/6851 , C12Q1/6813 , C12Q1/6865 , C12Q2545/101 , C12Q2537/143
Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
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4.发明授权Reaction mixtures for quantitative amplification and detection over a wide dynamic range 有权用于在宽动态范围内进行定量扩增和检测的反应混合物公开(公告)号：US09347098B2
公开(公告)日：2016-05-24
申请号：US14562922
申请日：2014-12-08
Applicant: Gen-Probe Incorporated
Inventor： Daniel L. Kacian , Kenneth A. Browne
CPC classification number: C12Q1/6851 , C12Q1/6813 , C12Q1/6865 , C12Q2545/101 , C12Q2537/143
Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
Abstract translation: 公开了使用单个扩增系统在单个容器中以不等比例制备可微分扩增子的组合物和方法。 选择可差分扩增子的数目及其彼此的比例以跨越扩增反应的所需线性动态范围，并适应用于确定产生的扩增子的量的测量系统的限制。 通过提供不等量的一个或多个扩增反应组分（例如，可区分的扩增寡核苷酸，NTP混合物中的天然和非天然NTP等）产生不等量的可区分的扩增子种类。 使用从检测动态范围内的一种或多种扩增子种检测产生的线性检测范围来确定测试样品中存在的靶核酸的量。
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5.发明申请METHODS FOR QUANTITATIVE AMPLIFICATION AND DETECTION OVER A WIDE DYNAMIC RANGE 有权用于定量放大和检测在宽动态范围内的方法公开(公告)号：US20140072974A1
公开(公告)日：2014-03-13
申请号：US14064427
申请日：2013-10-28
Applicant: Gen-Probe Incorporated
Inventor： Daniel L. Kacian , Kenneth A. Browne
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q1/6813 , C12Q1/6865 , C12Q2545/101 , C12Q2537/143
Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
Abstract translation: 公开了使用单个扩增系统在单个容器中以不等比例制备可微分扩增子的组合物和方法。 选择可差分扩增子的数目及其彼此的比例以跨越扩增反应的所需线性动态范围，并适应用于确定产生的扩增子的量的测量系统的限制。 通过提供不等量的一个或多个扩增反应组分（例如，可区分的扩增寡核苷酸，NTP混合物中的天然和非天然NTP等）产生不等量的可区分的扩增子种类。 使用从检测动态范围内的一种或多种扩增子种检测产生的线性检测范围来确定测试样品中存在的靶核酸的量。
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公开(公告)号：US20180216172A1
公开(公告)日：2018-08-02
申请号：US15843391
申请日：2017-12-15
Applicant: GEN-PROBE INCORPORATED
Inventor： Daniel L. Kacian , Kenneth A. Browne
IPC: C12Q1/6851 , C12Q1/6813 , C12Q1/6865
CPC classification number: C12Q1/6851 , C12Q1/6813 , C12Q1/6865 , C12Q2545/101 , C12Q2537/143
Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
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7.发明申请METHODS FOR QUANTITATIVE AMPLIFICATION AND DETECTION OVER A WIDE DYNAMIC RANGE 有权用于定量放大和检测在宽动态范围内的方法公开(公告)号：US20150093753A1
公开(公告)日：2015-04-02
申请号：US14562922
申请日：2014-12-08
Applicant: Gen-Probe Incorporated
Inventor： Daniel L. Kacian , Kenneth A. Browne
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q1/6813 , C12Q1/6865 , C12Q2545/101 , C12Q2537/143
Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
Abstract translation: 公开了使用单个扩增系统在单个容器中以不等比例制备可微分扩增子的组合物和方法。 选择可差分扩增子的数目及其彼此的比例以跨越扩增反应的所需线性动态范围，并适应用于确定产生的扩增子的量的测量系统的限制。 通过提供不等量的一个或多个扩增反应组分（例如，可区分的扩增寡核苷酸，NTP混合物中的天然和非天然NTP等）产生不等量的可区分的扩增子种类。 使用从检测动态范围内的一种或多种扩增子种检测产生的线性检测范围来确定测试样品中存在的靶核酸的量。
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