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Patent Agency Ranking
公开(公告)号：US10364268B2
公开(公告)日：2019-07-30
申请号：US14365449
申请日：2012-12-18
Applicant: GENENTECH, INC.
Inventor： Jerome Joseph Bill, Jr. , Arick Michael Brown , Christopher John Dowd , Brooke Ellen Thayer
Abstract: Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.
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公开(公告)号：US20150050275A1
公开(公告)日：2015-02-19
申请号：US14511673
申请日：2014-10-10
Applicant: GENENTECH, INC.
Inventor： Marc Wong , Jerome Joseph Bill, JR. , Arick Michael Brown , Glen Scott Giese , Judy Fay-Chen Hsii , Amy Lim , Josefine Persson , Asha Nandini Radhamohan , Maricel Rodriguez
IPC: C07K16/28 , A61K45/06 , A61K39/395
CPC classification number: C07K16/2863 , A61K39/39558 , A61K45/06 , C07K1/14 , C07K1/18 , C07K1/22 , C07K1/34 , C07K1/36 , C07K16/065 , C07K16/32 , C07K2317/24 , C07K2317/56
Abstract: Provided herein are methods of purifying anti-c-met antibodies, compositions and pharmaceutical formulations comprising purified anti-c-met antibodies, and methods of using the same.
Abstract translation: 本文提供了纯化抗c-met抗体的方法，包含纯化的抗c-met抗体的组合物和药物制剂及其使用方法。
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公开(公告)号：US11945837B2
公开(公告)日：2024-04-02
申请号：US16433763
申请日：2019-06-06
Applicant: Genentech, Inc.
Inventor： Jerome Joseph Bill, Jr. , Arick Michael Brown , Christopher John Dowd , Brooke Ellen Thayer
CPC classification number: C07K1/18 , C07K1/165 , C07K16/065
Abstract: Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.
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公开(公告)号：US20140348845A1
公开(公告)日：2014-11-27
申请号：US14365449
申请日：2012-12-18
Applicant: GENENTECH, INC.
Inventor： Jerome Joseph Bill, Jr. , Arick Michael Brown , Christopher John Dowd , Brooke Ellen Thayer
CPC classification number: C07K1/18 , C07K1/165 , C07K16/065
Abstract: Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.
Abstract translation: 提高用于纯化蛋白质的下游色谱步骤的效率的方法，包括：（a）使包含感兴趣的多肽和各种污染物的组合物通过离子交换膜，其中所述多肽和膜具有相反的电荷，在包含 缓冲液具有与多肽的pI足够不同的pH以增强多肽的电荷，以及有效地防止由缓冲离子屏蔽电荷的低离子强度，其导致膜结合多肽和至少一种污染物（ b）使离子交换膜过载，使得至少一种污染物保持与膜结合，而感兴趣的多肽主要在流出物中; （c）从包含感兴趣的多肽的离子交换膜收集流出物; （d）使包含所述多肽的膜流出物与之前的膜具有相似电荷的纯化步骤，和（e）从带电离子交换色谱纯化步骤的流出物中回收纯化的多肽。
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公开(公告)号：US20130129718A1
公开(公告)日：2013-05-23
申请号：US13681980
申请日：2012-11-20
Applicant: Genentech, Inc.
Inventor： Marc Wong , Jerome Joseph Bill, JR. , Arick Michael Brown , Glen Scott Giese , Judy Fay-Chen Hsii , Amy Lim , Josefine Persson , Asha Nandini Radhamohan , Maricel Rodriguez
CPC classification number: C07K16/2863 , A61K39/39558 , A61K45/06 , C07K1/14 , C07K1/18 , C07K1/22 , C07K1/34 , C07K1/36 , C07K16/065 , C07K16/32 , C07K2317/24 , C07K2317/56
Abstract: Provided herein are methods of purifying anti-c-met antibodies, compositions and pharmaceutical formulations comprising purified anti-c-met antibodies, and methods of using the same.
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