公开(公告)号：US10359419B2

公开(公告)日：2019-07-23

申请号：US14043887

申请日：2013-10-02

Applicant: General Electric Company

Inventor： Anthony John Murray ,  Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  Nandini Nagraj

IPC: G01N33/53 ,  G01N33/532 ,  G01N33/58

Abstract: A method of recovering a target from a sample is provided. The method comprises the adding a substrate coupled binding element to the sample comprising the target to form a substrate coupled binding element-target complex; precipitating the complex by changing one or more environmental conditions of the substrate and recovering the target and the substrate coupled binding-element under mild conditions.

公开(公告)号：US09828599B2

公开(公告)日：2017-11-28

申请号：US14030386

申请日：2013-09-18

Applicant: General Electric Company

Inventor： Nandini Nagraj ,  Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  John Richard Nelson

IPC: C40B30/04 ,  C12N15/10 ,  G01N33/53 ,  H04N1/32 ,  H04N1/40 ,  H04N1/60 ,  C12Q1/68

CPC classification number: C12N15/1034 ,  C12N15/1048 ,  C12Q1/6811 ,  C40B30/04 ,  G01N33/53 ,  G01N33/5308 ,  H04N1/32309 ,  H04N1/3232 ,  H04N1/32352 ,  H04N1/40 ,  H04N1/60 ,  H04N2201/0094 ,  H04N2201/3233 ,  H04N2201/327 ,  H04N2201/3271 ,  H04N2209/00 ,  C12Q2525/205 ,  C12Q2531/125 ,  C12Q2537/162 ,  C12Q2565/107

Abstract: Methods for selecting a binding-element are provided. The method comprised of different steps. A first mixture is formed using at least one target molecule and a plurality of oligomers, followed by incubating the first mixture to form a second mixture comprising at least one target-bound oligomer and at least one target-unbound oligomer. Then a first accelerator is added to cleave the target-unbound oligomer and the target-bound oligomer is separated from the target molecule. This is followed by addition of a second accelerator for ligation, and a third accelerator for amplification followed by sequencing and post sequence analysis to select the binding-element.

公开(公告)号：US20170267716A1

公开(公告)日：2017-09-21

申请号：US15071210

申请日：2016-03-16

Applicant: General Electric Company

Inventor： Anthony John Murray ,  Andrew David Pris ,  Erik Leeming Kvam ,  Nandini Nagraj ,  Eugene Pauling Boden ,  Ernest William Kovacs ,  Louisa Ruth Carr ,  Rui Chen ,  Tiberiu Mircea Siclovan ,  Kelly Scott Chichak

IPC: C07K1/36 ,  C07K16/00 ,  C12N9/74 ,  C12N15/115 ,  C07K14/705

CPC classification number: C07K1/36 ,  C07K1/32 ,  C07K14/70564 ,  C07K16/4291 ,  C12N9/6429 ,  C12Y304/21005

Abstract: Provided herein are aptamer-polymer conjugates which are responsive to environmental stimuli and are useful in selective purification of untagged target polypeptides.

公开(公告)号：US20170191992A1

公开(公告)日：2017-07-06

申请号：US15465970

申请日：2017-03-22

Applicant: General Electric Company

Inventor： Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  Nandini Nagraj ,  Anthony John Murray

IPC: G01N33/53 ,  G01N33/543

CPC classification number: G01N33/5306 ,  G01N33/5308 ,  G01N33/54306 ,  G01N33/54386

Abstract: A method of recovering a target from a sample is provided. The method of recovering the target follows different steps. The steps include providing a binding element, wherein the binding elements are immobilized on a solid support, adding the sample comprising the target to the binding element to form a binding element-target complex; washing the binding element-target complex; and eluting the target from the binding element-target complex. The system for reversible detection of target in a range from 2 to 1,000,000 bind/release cycles is also provided.

公开(公告)号：US20150093820A1

公开(公告)日：2015-04-02

申请号：US14043887

申请日：2013-10-02

Applicant: General Electric Company

Inventor： Anthony John Murray ,  Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  Nandini Nagraj

IPC: C12N15/115

CPC classification number: G01N33/5308 ,  G01N33/532 ,  G01N33/58

Abstract: A method of recovering a target from a sample is provided. The method comprises the adding a substrate coupled binding element to the sample comprising the target to form a substrate coupled binding element-target complex; precipitating the complex by changing one or more environmental conditions of the substrate and recovering the target and the substrate coupled binding-element under mild conditions.

Abstract translation: 提供了从样品中回收靶的方法。 该方法包括将底物耦合的结合元件添加到包含靶的样品以形成基底耦合的结合元件 - 目标复合物; 通过改变衬底的一个或多个环境条件并在温和条件下回收靶和衬底耦合的结合元件来沉淀复合物。

公开(公告)号：US20150093839A1

公开(公告)日：2015-04-02

申请号：US14043895

申请日：2013-10-02

Applicant: General Electric Company

Inventor： Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  Nandini Nagraj ,  Anthony John Murray

IPC: G01N33/53

CPC classification number: G01N33/5306 ,  G01N33/5308 ,  G01N33/54306 ,  G01N33/54386

Abstract: A method of recovering a target from a sample is provided. The method of recovering the target follows different steps. The steps include providing a binding element, wherein the binding elements are immobilized on a solid support, adding the sample comprising the target to the binding element to form a binding element-target complex; washing the binding element-target complex; and eluting the target from the binding element-target complex. The system for reversible detection of target in a range from 2 to 1,000,000 bind/release cycles is also provided.

Abstract translation: 提供了从样品中回收靶的方法。 恢复目标的方法遵循不同的步骤。 所述步骤包括提供结合元件，其中所述结合元件固定在固体支持物上，将包含所述靶的样品添加到所述结合元件以形成结合元件 - 目标复合物; 洗涤结合元素 - 目标复合物; 并从结合元件 - 目标复合物洗脱目标物。 还提供了在2至1,000,000个结合/释放循环范围内可目标检测靶的系统。

公开(公告)号：US20150080226A1

公开(公告)日：2015-03-19

申请号：US14030386

申请日：2013-09-18

Applicant: General Electric Company

Inventor： Nandini Nagraj ,  Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  John Richard Nelson

IPC: C12N15/10 ,  G01N33/53

CPC classification number: C12N15/1034 ,  C12N15/1048 ,  C12Q1/6811 ,  C40B30/04 ,  G01N33/53 ,  G01N33/5308 ,  H04N1/32309 ,  H04N1/3232 ,  H04N1/32352 ,  H04N1/40 ,  H04N1/60 ,  H04N2201/0094 ,  H04N2201/3233 ,  H04N2201/327 ,  H04N2201/3271 ,  H04N2209/00 ,  C12Q2525/205 ,  C12Q2531/125 ,  C12Q2537/162 ,  C12Q2565/107

Abstract: Methods for selecting a binding-element are provided. The method comprised of different steps. A first mixture is formed using at least one target molecule and a plurality of oligomers, followed by incubating the first mixture to form a second mixture comprising at least one target-bound oligomer and at least one target-unbound oligomer. Then a first accelerator is added to cleave the target-unbound oligomer and the target-bound oligomer is separated from the target molecule. This is followed by addition of a second accelerator for ligation, and a third accelerator for amplification followed by sequencing and post sequence analysis to select the binding-element.

Abstract translation: 提供了选择结合元件的方法。 该方法由不同的步骤组成。 使用至少一种靶分子和多个低聚物形成第一混合物，随后温育第一混合物以形成包含至少一种靶结合的低聚物和至少一种靶未结合的低聚物的第二混合物。 然后加入第一促进剂以切割目标未结合的寡聚物，并且靶向寡聚体与目标分子分离。 然后加入第二种用于连接的促进剂，和第三种用于扩增的加速剂，然后进行测序和后序列分析以选择结合元件。