公开(公告)号：US20230348865A1

公开(公告)日：2023-11-02

申请号：US17996921

申请日：2021-04-23

Applicant: Genomatica, Inc.

Inventor： Amit Mahendra SHAH ,  Deqiang ZHANG ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN ,  Russell Scott KOMOR ,  Harish NAGARAJAN ,  Sankha GHATAK ,  Toby Howard RICHARDSON

IPC: C12N9/02 ,  C12P13/00 ,  C12P7/04 ,  C12N15/52 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12N9/0008 ,  C12Y102/0103 ,  C12P13/001 ,  C12P7/04 ,  C12N15/52 ,  C12N9/1029 ,  C12Y203/01174 ,  C12Y102/01003 ,  C12N9/88 ,  C12Y402/01017 ,  C12N9/1096

Abstract: The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semi aldehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.