公开(公告)号：US20240294885A1

公开(公告)日：2024-09-05

申请号：US18707150

申请日：2022-10-26

Applicant: Genomatica, Inc.

Inventor： Amit Mahendra SHAH ,  Deqiang ZHANG ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN ,  Jinel SHAH ,  Justin Robert COLQUITT

IPC: C12N9/10 ,  C12N9/02 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P13/00

CPC classification number: C12N9/1096 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P13/001 ,  C12Y102/01003 ,  C12Y102/0103 ,  C12Y203/01174

Abstract: The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semialdehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.

公开(公告)号：US20230348865A1

公开(公告)日：2023-11-02

申请号：US17996921

申请日：2021-04-23

Applicant: Genomatica, Inc.

Inventor： Amit Mahendra SHAH ,  Deqiang ZHANG ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN ,  Russell Scott KOMOR ,  Harish NAGARAJAN ,  Sankha GHATAK ,  Toby Howard RICHARDSON

IPC: C12N9/02 ,  C12P13/00 ,  C12P7/04 ,  C12N15/52 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12N9/0008 ,  C12Y102/0103 ,  C12P13/001 ,  C12P7/04 ,  C12N15/52 ,  C12N9/1029 ,  C12Y203/01174 ,  C12Y102/01003 ,  C12N9/88 ,  C12Y402/01017 ,  C12N9/1096

Abstract: The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semi aldehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.

公开(公告)号：US20230167468A1

公开(公告)日：2023-06-01

申请号：US17995778

申请日：2021-04-13

Applicant: GENOMATICA, INC.

Inventor： Deqiang ZHANG ,  Jamison Parker HUDDLESTON ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN

IPC: C12P7/42 ,  C12N9/02 ,  C12P17/06 ,  C12P7/22

CPC classification number: C12P7/42 ,  C12N9/0004 ,  C12Y121/03008 ,  C12Y121/03007 ,  C12P17/06 ,  C12P7/22

Abstract: The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase Δ9-tetrahydrocannabinolic acid synthase (THCAS), comprising three alpha helices (αA, αB and αC) where a disulfide bond is not formed between alpha helix αA and alpha helix αC, wherein the non-natural cannabinoid synthase catalyzes the oxidative cyclization of cannabigerolic acid (CBGA) into a cannabinoid. The invention further relates to a non-natural Δ9-tetrahydrocannabinolic acid synthase (THCAS), a non-natural cannabidiolic acid synthase (CBDAS), and a non-natural cannabichromenic acid synthase (CBCAS) comprising at least one amino acid variation as compared to a wild type THCAS, CBDAS, or CBCAS, respectively, comprising three alpha helices (αA, αB and αC) and wherein a disulfide bond is not formed between alpha helix αA and alpha helix αC. The invention also relates to a nucleic acid, expression construct, and engineered cell for making the non-natural THCAS, CBDAS, and/or CBCAS. Also provided are compositions comprising the non-natural THCAS, CBDAS, and/or CBCAS; isolated non-natural THCAS, CBDAS, and/or CBCAS enzymes; methods of making the isolated enzymes; cell extracts comprising cannabinoids; and methods of making cannabinoids.