公开(公告)号：US20090178153A1

公开(公告)日：2009-07-09

申请号：US12243731

申请日：2008-10-01

申请人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demitri Vassilatis ,  Hongkui Zeng

发明人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demitri Vassilatis ,  Hongkui Zeng

IPC分类号： A01K67/027 ,  C12Q1/68 ,  A61K31/7088 ,  G01N33/53 ,  C12N15/00 ,  C12N5/10 ,  A61K49/00

CPC分类号： G01N33/74 ,  A61K38/00 ,  C07K14/705 ,  C07K14/723 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N2333/726 ,  G01N2500/04 ,  G01N2500/10 ,  Y02A50/463 ,  Y02A50/469 ,  Y02A50/475 ,  Y02A50/48

摘要： The present invention provides GPCR polypeptides and polynucleotides, recombinant materials, and transgenic mice, as well as methods for their production. The polypeptides and polynucleotides are useful, for example, in methods of diagnosis and treatment of diseases and disorders. The invention also provides methods for identifying compounds (e.g., agonists or antagonists) using the GPCR polypeptides and polynucleotides of the invention, and for treating conditions associated with GPCR dysfunction with the GPCR polypeptides, polynucleotides, or identified compounds. The invention also provides diagnostic assays for detecting diseases or disorders associated with inappropriate GPCR activity or levels.

摘要翻译： 本发明提供GPCR多肽和多核苷酸，重组材料和转基因小鼠，以及其生产方法。 多肽和多核苷酸可用于例如诊断和治疗疾病和病症的方法。 本发明还提供使用本发明的GPCR多肽和多核苷酸鉴定化合物（例如激动剂或拮抗剂）以及用GPCR多肽，多核苷酸或鉴定的化合物治疗与GPCR功能障碍相关的病症的方法。 本发明还提供用于检测与不适当GPCR活性或水平相关的疾病或病症的诊断测定。

公开(公告)号：US20130247233A1

公开(公告)日：2013-09-19

申请号：US13796312

申请日：2013-03-12

申请人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

发明人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

IPC分类号： C12Q1/68 ,  C07K14/705

CPC分类号： G01N33/74 ,  A61K38/00 ,  C07K14/705 ,  C07K14/723 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N2333/726 ,  G01N2500/04 ,  G01N2500/10 ,  Y02A50/463 ,  Y02A50/469 ,  Y02A50/475 ,  Y02A50/48

摘要： The present invention provides GPCR polypeptides and polynucleotides, recombinant materials, and transgenic mice, as well as methods for their production. The polypeptides and polynucleotides are useful, for example, in methods of diagnosis and treatment of diseases and disorders. The invention also provides methods for identifying compounds (e.g., agonists or antagonists) using the GPCR polypeptides and polynucleotides of the invention, and for treating conditions associated with GPCR dysfunction with the GPCR polypeptides, polynucleotides, or identified compounds. The invention also provides diagnostic assays for detecting diseases or disorders associated with inappropriate GPCR activity or levels.

公开(公告)号：US20110185439A1

公开(公告)日：2011-07-28

申请号：US12970094

申请日：2010-12-16

申请人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

发明人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

IPC分类号： G01N33/48 ,  A01K67/027 ,  C12N15/87 ,  C12N5/10 ,  C07K14/47 ,  C07H21/04 ,  A61K31/7088 ,  A61P25/00 ,  A61P3/00 ,  C12Q1/68 ,  A61P9/00 ,  A61P17/00 ,  G01N33/53

CPC分类号： G01N33/74 ,  A61K38/00 ,  C07K14/705 ,  C07K14/723 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N2333/726 ,  G01N2500/04 ,  G01N2500/10 ,  Y02A50/463 ,  Y02A50/469 ,  Y02A50/475 ,  Y02A50/48

摘要： The present invention provides GPCR polypeptides and polynucleotides, recombinant materials, and transgenic mice, as well as methods for their production. The polypeptides and polynucleotides are useful, for example, in methods of diagnosis and treatment of diseases and disorders. The invention also provides methods for identifying compounds (e.g., agonists or antagonists) using the GPCR polypeptides and polynucleotides of the invention, and for treating conditions associated with GPCR dysfunction with the GPCR polypeptides, polynucleotides, or identified compounds. The invention also provides diagnostic assays for detecting diseases or disorders associated with inappropriate GPCR activity or levels.

摘要翻译： 本发明提供GPCR多肽和多核苷酸，重组材料和转基因小鼠，以及其生产方法。 多肽和多核苷酸可用于例如诊断和治疗疾病和病症的方法。 本发明还提供使用本发明的GPCR多肽和多核苷酸鉴定化合物（例如激动剂或拮抗剂）以及用GPCR多肽，多核苷酸或鉴定的化合物治疗与GPCR功能障碍相关的病症的方法。 本发明还提供用于检测与不适当GPCR活性或水平相关的疾病或病症的诊断测定。

公开(公告)号：US08999654B2

公开(公告)日：2015-04-07

申请号：US12916227

申请日：2010-10-29

申请人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

发明人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. McIlwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

IPC分类号： G01N33/566 ,  C07K14/72 ,  C12Q1/68 ,  C07K14/705 ,  A61K38/00

CPC分类号： A01K67/0278 ,  A01K2217/00 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  A61K49/0008 ,  C07K14/723 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/5023 ,  G01N2333/726 ,  G01N2800/04 ,  G01N2800/28 ,  G01N2800/50

摘要： The present invention provides GPCR polypeptides and polynucleotides, recombinant materials, and transgenic mice, as well as methods for their production. The polypeptides and polynucleotides are useful, for example, in methods of diagnosis and treatment of diseases and disorders. The invention also provides methods for identifying compounds (e.g., agonists or antagonists) using the GPCR polypeptides and polynucleotides of the invention, and for treating conditions associated with GPCR dysfunction with the GPCR polypeptides, polynucleotides, or identified compounds. The invention also provides diagnostic assays for detecting diseases or disorders associated with inappropriate GPCR activity or levels.

摘要翻译： 本发明提供GPCR多肽和多核苷酸，重组材料和转基因小鼠，以及其生产方法。 多肽和多核苷酸可用于例如诊断和治疗疾病和病症的方法。 本发明还提供使用本发明的GPCR多肽和多核苷酸鉴定化合物（例如激动剂或拮抗剂）以及用GPCR多肽，多核苷酸或鉴定的化合物治疗与GPCR功能障碍相关的病症的方法。 本发明还提供用于检测与不适当GPCR活性或水平相关的疾病或病症的诊断测定。

公开(公告)号：US20080260744A1

公开(公告)日：2008-10-23

申请号：US11940917

申请日：2007-11-15

申请人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. Mcllwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

发明人： George A. Gaitanaris ,  John E. Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie L. Mcllwain ,  Maria N. Pavlova ,  Demetri Vassilatis ,  Hongkui Zeng

IPC分类号： A61K39/395 ,  A61K31/70 ,  C12Q1/68 ,  C12Q1/02 ,  A01K67/027 ,  G01N33/00 ,  C12N5/06 ,  G01N33/569 ,  A61P25/00

CPC分类号： A01K67/0278 ,  A01K2217/00 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  A61K49/0008 ,  C07K14/723 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/5023 ,  G01N2333/726 ,  G01N2800/04 ,  G01N2800/28 ,  G01N2800/50

摘要： The present invention provides GPCR polypeptides and polynucleotides, recombinant materials, and transgenic mice, as well as methods for their production. The polypeptides and polynucleotides are useful, for example, in methods of diagnosis and treatment of diseases and disorders. The invention also provides methods for identifying compounds (e.g., agonists or antagonists) using the GPCR polypeptides and polynucleotides of the invention, and for treating conditions associated with GPCR dysfunction with the GPCR polypeptides, polynucleotides, or identified compounds. The invention also provides diagnostic assays for detecting diseases or disorders associated with inappropriate GPCR activity or levels.

摘要翻译： 本发明提供GPCR多肽和多核苷酸，重组材料和转基因小鼠，以及其生产方法。 多肽和多核苷酸可用于例如诊断和治疗疾病和病症的方法。 本发明还提供使用本发明的GPCR多肽和多核苷酸鉴定化合物（例如激动剂或拮抗剂）以及用GPCR多肽，多核苷酸或鉴定的化合物治疗与GPCR功能障碍相关的病症的方法。 本发明还提供用于检测与不适当GPCR活性或水平相关的疾病或病症的诊断测定。

公开(公告)号：US20060134109A1

公开(公告)日：2006-06-22

申请号：US10527265

申请日：2003-09-09

申请人： George Gaitanaris ,  John Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie McIlwain ,  Maria Pavlova ,  Demitri Vassilatis ,  Hongkui Zeng

发明人： George Gaitanaris ,  John Bergmann ,  Alexander Gragerov ,  John Hohmann ,  Fusheng Li ,  Linda Madisen ,  Kellie McIlwain ,  Maria Pavlova ,  Demitri Vassilatis ,  Hongkui Zeng

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  A61K39/395 ,  C07K14/705

CPC分类号： G01N33/74 ,  A61K38/00 ,  C07K14/705 ,  C07K14/723 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N2333/726 ,  G01N2500/04 ,  G01N2500/10 ,  Y02A50/463 ,  Y02A50/469 ,  Y02A50/475 ,  Y02A50/48

摘要： The present invention provides GPCR polypeptides and polynucleotides, recombinant materials, and transgenic mice, as well as methods for their production. The polypeptides and polynucleotides are useful, for example, in methods of diagnosis and treatment of diseases and disorders. The invention also provides methods for identifying compounds (e.g., agonists or antagonists) using the GPCR polypeptides and polynucleotides of the invention, and for treating conditions associated with GPCR dysfunction with the GPCR polypeptides, polynucleotides, or identified compounds. The invention also provides diagnostic assays for detecting diseases or disorders associated with inappropriate GPCR activity or levels.

摘要翻译： 本发明提供GPCR多肽和多核苷酸，重组材料和转基因小鼠，以及其生产方法。 多肽和多核苷酸可用于例如诊断和治疗疾病和病症的方法。 本发明还提供使用本发明的GPCR多肽和多核苷酸鉴定化合物（例如激动剂或拮抗剂）以及用GPCR多肽，多核苷酸或鉴定的化合物治疗与GPCR功能障碍相关的病症的方法。 本发明还提供用于检测与不适当GPCR活性或水平相关的疾病或病症的诊断测定。

公开(公告)号：US20120027792A1

公开(公告)日：2012-02-02

申请号：US13190969

申请日：2011-07-26

申请人： George N. Pavlakis ,  Alexander Gragerov ,  Barbara K. Felber

发明人： George N. Pavlakis ,  Alexander Gragerov ,  Barbara K. Felber

IPC分类号： A61K39/21 ,  A61P31/12 ,  A61P31/18 ,  A61K39/12

CPC分类号： C12N15/62 ,  A61K39/00 ,  A61K2039/5256 ,  C07K2319/02 ,  C07K2319/40 ,  C07K2319/75

摘要： The invention relates to nucleic acids (such as DNA immunization plasmids), encoding fusion proteins containing a destabilizing amino acid sequence attached to an amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence. The invention also relates to nucleic acids encoding secreted fusion proteins, such as those containing chemokines or cytokines, and an attached amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased as a result of being attached to the secretory sequence. The invention also relates methods of increasing the immunogenicity of the encoded proteins for use as vaccines or in gene therapy.

摘要翻译： 本发明涉及编码融合蛋白的核酸（例如DNA免疫质粒），其含有与感兴趣的氨基酸序列连接的去稳定性氨基酸序列的融合蛋白，其中感兴趣的氨基酸序列的免疫原性通过存在 不稳定氨基酸序列。 本发明还涉及编码分泌的融合蛋白的核酸，例如含有趋化因子或细胞因子的核酸，以及所关注的附着的氨基酸序列，其中所附的氨基酸序列的免疫原性因附着于 分泌序列。 本发明还涉及提高用于疫苗或基因治疗的编码蛋白的免疫原性的方法。

公开(公告)号：US20090227664A1

公开(公告)日：2009-09-10

申请号：US12426901

申请日：2009-04-20

申请人： George N. Pavlakis ,  Alexander Gragerov ,  Barbara K. Felber

发明人： George N. Pavlakis ,  Alexander Gragerov ,  Barbara K. Felber

IPC分类号： A61K31/711 ,  C12N15/85 ,  A61P31/20

CPC分类号： C12N15/62 ,  A61K39/00 ,  A61K2039/5256 ,  C07K2319/02 ,  C07K2319/40 ,  C07K2319/75

摘要： The invention relates to nucleic acids (such as DNA immunization plasmids), encoding fusion proteins containing a destabilizing amino acid sequence attached to an amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence. The invention also relates to nucleic acids encoding secreted fusion proteins, such as those containing chemokines or cytokines, and an attached amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased as a result of being attached to the secretory sequence. The invention also relates methods of increasing the immunogenicity of the encoded proteins for use as vaccines or in gene therapy.

摘要翻译： 本发明涉及编码融合蛋白的核酸（例如DNA免疫质粒），其含有与感兴趣的氨基酸序列连接的去稳定性氨基酸序列的融合蛋白，其中感兴趣的氨基酸序列的免疫原性通过存在 不稳定氨基酸序列。 本发明还涉及编码分泌的融合蛋白的核酸，例如含有趋化因子或细胞因子的核酸，以及所关注的附着的氨基酸序列，其中所附的氨基酸序列的免疫原性因附着于 分泌序列。 本发明还涉及提高用于疫苗或基因治疗的编码蛋白的免疫原性的方法。