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92 条记录 (耗时 0.026 秒)

    Pressure-mediated binding of biomolecular complexes
    1.
    发明授权
    Pressure-mediated binding of biomolecular complexes 有权
    压力介导的生物分子复合物的结合

    公开(公告)号：US06635469B1

    公开(公告)日：2003-10-21

    申请号：US09214250

    申请日：1999-08-20

    申请人： Gerald J. Litt ,  James A. Laugharn ,  David J. Green

    发明人： Gerald J. Litt ,  James A. Laugharn ,  David J. Green

    IPC分类号： G01N3353

    CPC分类号： G01N33/5306 ,  B01J3/00 ,  C11B7/005 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543

    摘要： The invention relates to (1) pressure-mediated dissociation of an analyte complexed with an endogenous binding partner to enable detection of a complex formed from the analyte and an exogenous binding factor, (2) pressure-mediated association of an analyte and an exogenous binding partner to enable more rapid and/or more sensitive detection of an analyte, and (3) pressure-mediated association and dissociation of biomolecular complexes to enable separation of one biomolecule from a complex mixture. Pressure can be used to improve assays by dissociating endogenous analyte complexes and improving assay speed and sensitivity by associating the analyte molecules with exogenously supplied binding partners. Pressure can also be used to improve the separation of compounds from contaminated mixtures. Methods of assaying an analyte in a sample having an endogenous complex between the analyte and an endogenous sample component include dissociating the analyte from the endogenous component using pressure and reacting the analyte with an exogenously supplied specific binding reagent to determine complexation between the analyte and the binding reagent.

    摘要翻译： 本发明涉及（1）与内源结合配偶体复合的分析物的压力介导的解离，以能够检测由分析物形成的复合物和外源性结合因子，（2）压力介导的分析物缔合和外源结合 合作伙伴能够更快速和/或更敏感地检测分析物，和（3）压力介​​导的生物分子复合物的缔合和解离，以使一个生物分子与复杂混合物分离。 压力可用于通过解离内源性分析物复合物来改进测定，并通过将分析物分子与外源提供的结合配偶体相关联来提高测定速度和灵敏度。 也可以使用压力来改善化合物与污染混合物的分离。测定样品中分析物与分析物与内源性样品成分之间的内源性复合物的方法包括使用压力将分析物与内源成分解离，并使分析物与 外源供应的特异性结合试剂，以确定分析物与结合试剂之间的复合。

    Pressure-controlled nucleic acid hybridization
    3.
    发明授权
    Pressure-controlled nucleic acid hybridization 失效
    压力控制核酸杂交

    公开(公告)号：US06258534B1

    公开(公告)日：2001-07-10

    申请号：US09035652

    申请日：1998-03-05

    申请人： James A. Laugharn, Jr. ,  David J. Green ,  Robert A. Hess

    发明人： James A. Laugharn, Jr. ,  David J. Green ,  Robert A. Hess

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6813 ,  C12Q2527/113

    摘要： A method of hybridizing a first nucleic acid to a second nucleic acid at least partially complementary to the first nucleic acid by (1) providing a sample vessel and pressure controller for the vessel; and (2) contacting the first and second nucleic acids within the vessel at a pressure above ambient pressure that is effective to enhance hybridization of the first and second nucleic acids.

    摘要翻译： （1）提供用于血管的样品容器和压力控制器的将第一核酸与至少部分与第一核酸互补的第二核酸杂交的方法; 和（2）在高于环境压力的压力下使容器内的第一和第二核酸接触，其有效增强第一和第二核酸的杂交。

    Pressure-controlled nucleic acid hybridization
    4.
    发明授权
    Pressure-controlled nucleic acid hybridization 有权
    压力控制核酸杂交

    公开(公告)号：US06753169B2

    公开(公告)日：2004-06-22

    申请号：US09901297

    申请日：2001-07-09

    申请人： Robert A. Hess ,  James A. Laugharn, Jr. ,  David J. Green

    发明人： Robert A. Hess ,  James A. Laugharn, Jr. ,  David J. Green

    IPC分类号： C12P1934

    CPC分类号： C12Q1/6813 ,  C12Q2527/113

    摘要： A method of hybridizing a first nucleic acid to a second nucleic acid at least partially complementary to the first nucleic acid by (1) providing a sample vessel and pressure controller for the vessel; and (2) contacting the first and second nucleic acids within the vessel at a pressure above ambient pressure that is effective to enhance hybridization of the first and second nucleic acids.

    摘要翻译： （1）提供用于血管的样品容器和压力控制器的将第一核酸与至少部分与第一核酸互补的第二核酸杂交的方法; 和（2）在高于环境压力的压力下使容器内的第一和第二核酸接触，其有效增强第一和第二核酸的杂交。

    Extended fill inflation apparatus
    6.
    发明授权
    Extended fill inflation apparatus 失效
    延长填充充气装置

    公开(公告)号：US07641230B2

    公开(公告)日：2010-01-05

    申请号：US11153694

    申请日：2005-06-15

    申请人： Michael R. Schramm ,  David J. Green

    发明人： Michael R. Schramm ,  David J. Green

    IPC分类号： B60R21/26

    CPC分类号： B60R21/268 ,  B60R21/274 ,  B60R2021/0018 ,  B60R2021/2685

    摘要： Disclosed are methods, systems, and apparatus for inflating airbags. In one embodiment, an initiator is deployed to open an exit gas port, through which the airbag is inflated. The airbag is primarily inflated via gas from a first gas chamber and is substantially maintained in an inflated state for a predetermined period of time primarily via gas from a second gas chamber. In one embodiment, an airbag inflator is provided which comprises a first gas chamber, a second gas chamber, and an intermediate chamber assembly. The intermediate chamber assembly comprises a first gas port positioned adjacent to and in fluid communication with the first gas chamber and a second gas port positioned adjacent to and in fluid communication with the second gas chamber. The inflator is configured to allow a greater flow rate of gas through the first gas port upon deployment than through the second gas port.

    摘要翻译： 公开了用于充气安全气囊的方法，系统和装置。 在一个实施例中，启动器被展开以打开出口气体端口，气囊膨胀通过该出口气体端口。 气囊主要通过来自第一气室的气体膨胀，并且主要通过来自第二气室的气体基本保持在充气状态一段预定时间。 在一个实施例中，提供一种气囊充气机，其包括第一气体室，第二气体室和中间室组件。 中间室组件包括邻近第一气室定位并且与第一气室流体连通的第一气体端口和邻近第二气体室并与第二气体室流体连通地定位的第二气体端口。 充气机配置成允许在部署时气体通过第一气体端口的流速比通过第二气体端口更大。

    Airbag tether release mechanism
    8.
    发明授权
    Airbag tether release mechanism 有权
    安全气囊系绳释放机构

    公开(公告)号：US07419184B2

    公开(公告)日：2008-09-02

    申请号：US11154126

    申请日：2005-06-16

    申请人： David J. Green ,  Michael P. Jordan ,  Brent A. Parks

    发明人： David J. Green ,  Michael P. Jordan ,  Brent A. Parks

    IPC分类号： B60R21/16

    CPC分类号： B60R21/2338 ,  B60R21/231 ,  B60R21/26 ,  B60R2021/161 ,  B60R2021/23386

    摘要： Disclosed are embodiments of an airbag tether release mechanism for use with airbag inflation systems. In one embodiment, the mechanism includes an initiator positioned within a housing. The initiator is configured such that it provides an inherent seal to prevent the external release of pyrotechnic residues upon deployment. The device may also include a cutter operatively connected with the initiator such that deployment of the initiator actuates the cutter and a tether restraint structure configured to secure a tether until the tether is released by actuation of the cutter.

    摘要翻译： 公开了用于气囊充气系统的气囊系链释放机构的实施例。 在一个实施例中，该机构包括位于壳体内的起动器。 启动器被配置为使得其提供固有密封以防止在部署时烟火残余物的外部释放。 该装置还可以包括与起动器可操作地连接的切割器，使得起始器的展开促使切割器和构造成固定系绳的系绳约束结构，直到通过切割器的致动而释放系绳。

    Techniques for JEDEC file information integrity and preservation of device programming specifications
    9.
    发明授权
    Techniques for JEDEC file information integrity and preservation of device programming specifications 失效
    JEDEC文件信息完整性和设备编程规范保存技术

    公开(公告)号：US07043495B1

    公开(公告)日：2006-05-09

    申请号：US09916453

    申请日：2001-07-27

    申请人： David J. Green ,  Sungyong Pak ,  Fangyuan Nan

    发明人： David J. Green ,  Sungyong Pak ,  Fangyuan Nan

    IPC分类号： G06F17/30

    CPC分类号： G06F17/5054 ,  Y10S707/99931 ,  Y10S707/99939 ,  Y10S707/99943

    摘要： A method of generating a file suitable for programming a programmable logic device. The method generally comprises the steps of (A) generating a programming item from a plurality of parameters that define a program for the programmable logic device, (B) storing the programming item in a programming field of the file in response to generating, and (C) storing at least one of the parameters in a non-programming field of the file.

    摘要翻译： 一种产生适于编程可编程逻辑器件的文件的方法。 该方法通常包括以下步骤：（A）从定义可编程逻辑器件的程序的多个参数生成编程项目，（B）响应于生成将编程项目存储在文件的编程字段中，并且（ C）将至少一个参数存储在文件的非编程字段中。

    Biaxial flow inflator with independently adjusted gas orifices
    10.
    发明授权
    Biaxial flow inflator with independently adjusted gas orifices 失效
    双轴流量充气机，独立调整气体孔

    公开(公告)号：US06854763B2

    公开(公告)日：2005-02-15

    申请号：US10115857

    申请日：2002-06-06

    申请人： Paul Dinsdale ,  David J. Green ,  Karl K. Rink

    发明人： Paul Dinsdale ,  David J. Green ,  Karl K. Rink

    IPC分类号： B60R21/16 ,  B60R21/26 ,  B60R21/264 ,  B60R21/268

    CPC分类号： B60R21/26 ,  B60R21/232 ,  B60R2021/2612 ,  B60R2021/2633 ,  B60R2021/2685

    摘要： An airbag or inflatable curtain inflator for producing two differing gas flows is disposed. The inflator has a gas chamber, a first orifice, and a second orifice. The first orifice has a different effective cross-sectional area than the second orifice. The two different cross-sectional areas allow the first orifice to produce a mass flow rate of gas that is different from the second orifice. The mass flow rates of gas may be related to the volume of an inflatable curtain into which the gas is ejected. Thus, two differently sized volumes of an inflatable curtain may be simultaneously inflated with a single inflator.

    摘要翻译： 设置用于产生两种不同气体流的气囊或充气式帘式充气机。 充气机具有气室，第一孔和第二孔。 第一孔具有与第二孔不同的有效横截面面积。 两个不同的横截面区域允许第一孔产生不同于第二孔的气体的质量流率。 气体的质量流量可能与气体喷射到其中的可充气窗帘的体积有关。 因此，两个不同大小的体积的充气窗帘可以用单个充气机同时充气。