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1.发明授权公开(公告)号：US07977056B2
公开(公告)日：2011-07-12
申请号：US12464832
申请日：2009-05-12
申请人： Gerardo Vicente Toledo , Toby Howard Richardson , Ulrich Stingl , Eric J. Mathur , J. Craig Venter
发明人： Gerardo Vicente Toledo , Toby Howard Richardson , Ulrich Stingl , Eric J. Mathur , J. Craig Venter
CPC分类号： E21B43/006 , C09K8/582 , C12N1/20 , C12P5/023 , Y02E50/343
摘要： The present invention describes methods of identifying stimulants for the biogenic production of methane in hydrocarbon-bearing formations. Methods involve the use of microbial nucleic acid sequence information for the determination of gene products that are enzymes in a variety of pathways involved in the conversion of hydrocarbons to methane. Enzymes and stimulants identified by invention methods can be used in processes for enhancing biogenic methane production, for example, by addition to coal seams and coalbed methane wells.
摘要翻译： 本发明描述了鉴定含烃地层中甲烷生物生成的兴奋剂的方法。 方法涉及使用微生物核酸序列信息来确定作为将烃转化成甲烷的各种途径中的酶的基因产物。 通过发明方法鉴定的酶和兴奋剂可用于增强生物甲烷生产的方法，例如通过加入煤层和煤层气井。
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2.发明授权公开(公告)号：US08448702B2
公开(公告)日：2013-05-28
申请号：US13177429
申请日：2011-07-06
申请人： Gerardo Vicente Toledo , Toby Howard Richardson , Ulrich Stingl , Eric J. Mathur , J. Craig Venter
发明人： Gerardo Vicente Toledo , Toby Howard Richardson , Ulrich Stingl , Eric J. Mathur , J. Craig Venter
CPC分类号： E21B43/006 , C09K8/582 , C12N1/20 , C12P5/023 , Y02E50/343
摘要： The present invention describes methods of identifying stimulants for the biogenic production of methane in hydrocarbon-bearing formations. Methods involve the use of microbial nucleic acid sequence information for the determination of gene products that are enzymes in a variety of pathways involved in the conversion of hydrocarbons to methane. Enzymes and stimulants identified by invention methods can be used in processes for enhancing biogenic methane production, for example, by addition to coal seams and coalbed methane wells.
摘要翻译： 本发明描述了鉴定含烃地层中甲烷生物生成的兴奋剂的方法。 方法涉及使用微生物核酸序列信息来确定作为将烃转化成甲烷的各种途径中的酶的基因产物。 通过发明方法鉴定的酶和兴奋剂可用于增强生物甲烷生产的方法，例如通过加入煤层和煤层气井。
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3.发明申请公开(公告)号：US20110277991A1
公开(公告)日：2011-11-17
申请号：US13177429
申请日：2011-07-06
申请人： Gerardo Vicente Toledo , Toby Howard Richardson , Ulrich Stingl , Eric J. Mathur , J. Craig Venter
发明人： Gerardo Vicente Toledo , Toby Howard Richardson , Ulrich Stingl , Eric J. Mathur , J. Craig Venter
CPC分类号： E21B43/006 , C09K8/582 , C12N1/20 , C12P5/023 , Y02E50/343
摘要： The present invention describes methods of identifying stimulants for the biogenic production of methane in hydrocarbon-bearing formations. Methods involve the use of microbial nucleic acid sequence information for the determination of gene products that are enzymes in a variety of pathways involved in the conversion of hydrocarbons to methane. Enzymes and stimulants identified by invention methods can be used in processes for enhancing biogenic methane production, for example, by addition to coal seams and coalbed methane wells.
摘要翻译： 本发明描述了鉴定含烃地层中甲烷生物生成的兴奋剂的方法。 方法涉及使用微生物核酸序列信息来确定作为将烃转化成甲烷的各种途径中的酶的基因产物。 通过发明方法鉴定的酶和兴奋剂可用于增强生物甲烷生产的方法，例如通过加入煤层和煤层气井。
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4.发明授权公开(公告)号：US09556413B2
公开(公告)日：2017-01-31
申请号：US12532403
申请日：2008-03-21
摘要： The invention described below relates to an enclosed cell sorting device and methods of using the device. The device is constructed so that the entire process of cell sorting can be conducted under fully anaerobic conditions to retain viability of anaerobic cells before, during, and after cell sorting. This is accomplished by creating an anaerobic atmosphere for the high speed cell sorter and all its components and by the use of airlocks that allow the introduction of anaerobic containers into the chamber containing the sample.
摘要翻译： 下面描述的本发明涉及封闭的细胞分选装置和使用该装置的方法。 构建该装置使得整个细胞分选过程可以在完全厌氧条件下进行，以在细胞分选之前，期间和之后保留厌氧细胞的活力。 这是通过为高速细胞分选器及其所有组分产生厌氧气氛以及通过使用允许将厌氧容器引入含有样品的室的气闸实现的。
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