公开(公告)号：US20110296543A1

公开(公告)日：2011-12-01

申请号：US12324436

申请日：2008-11-26

Applicant: Hwai Chang ,  Eric J. Mathur ,  Michelle Cayouette ,  Dan E. Robertson ,  Philip Hugenholtz ,  Falk Warnecke ,  Jared R. Leadbetter ,  Natalia Ivanova ,  Peter Luginbuhl ,  Don Hutchison

Inventor： Hwai Chang ,  Eric J. Mathur ,  Michelle Cayouette ,  Dan E. Robertson ,  Philip Hugenholtz ,  Falk Warnecke ,  Jared R. Leadbetter ,  Natalia Ivanova ,  Peter Luginbuhl ,  Don Hutchison

IPC: A01K67/00

CPC classification number: C12Y302/01015 ,  C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01039 ,  C12Y302/01067 ,  C12Y302/01082

Abstract: The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. Polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to a biofuel (e.g., a bioethanol, biomethanol, biopropanol or biobutanol, a biodiesel, etc.), biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.

Abstract translation: 本发明提供了多肽，包括酶，结构蛋白和结合蛋白，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明的多肽，包括酶和抗体以及核酸可用于工业，实验，食品和饲料加工，营养和药物应用中，例如用于食品和饲料添加剂，着色剂，中性药物，化妆品和药物需求。 本发明的多肽可用于食品加工，酿造，浴添加剂，酒精生产，肽合成，对映选择性，皮革行业中的皮革制备，废物管理和动物降解，摄影工业中的银回收，医疗，丝绸脱胶， 生物膜降解，生物燃料转化为生物燃料（例如生物乙醇，生物甲醇，生物丙醇或生物丁醇，生物柴油等），生物防治剂，抗微生物剂和消毒剂，个人护理和化妆品，生物技术试剂，玉米湿法研磨和药物如 消化助剂和抗炎（抗炎）药剂。

公开(公告)号：US20100047392A1

公开(公告)日：2010-02-25

申请号：US12567550

申请日：2009-09-25

Applicant: Walter Callen ,  Toby Richardson ,  Gerhard Frey ,  Jay M. Short ,  Eric J. Mathur ,  Kevin A. Gray ,  Janne S. Kerovuo ,  Malgorzata Slupska

Inventor： Walter Callen ,  Toby Richardson ,  Gerhard Frey ,  Jay M. Short ,  Eric J. Mathur ,  Kevin A. Gray ,  Janne S. Kerovuo ,  Malgorzata Slupska

IPC: C12N9/26 ,  C12P19/14 ,  C11D3/386 ,  D06M16/00 ,  C13K1/06 ,  C09K8/20 ,  D21C5/02

CPC classification number: C12N9/2417 ,  A21D8/042 ,  C12N9/2414 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/16 ,  Y02E50/17

Abstract: The invention relates to alpha amylases and to polynucleotides and polypeptides encoding the alpha amylases. In addition, methods of using the alpha amylases are also provided. The alpha amylases have increased activity and stability at acidic, neutral and alkaline pH and at increased temperatures.

Abstract translation: 本发明涉及α-淀粉酶和编码α-淀粉酶的多核苷酸和多肽。 此外，还提供了使用α-淀粉酶的方法。 在酸性，中性和碱性pH和升高的温度下，α-淀粉酶具有增加的活性和稳定性。

公开(公告)号：US07465571B1

公开(公告)日：2008-12-16

申请号：US09914543

申请日：1997-05-22

Applicant: David Lam ,  Eric J. Mathur

Inventor： David Lam ,  Eric J. Mathur

IPC: C12N9/42 ,  C12P19/34 ,  C07K1/00 ,  D06M16/00 ,  C11D3/00 ,  C07H21/04 ,  A23L1/31

CPC classification number: C12N9/2437 ,  C12P19/14 ,  C12Y302/01004

Abstract: In one aspect, the invention provides a purified thermostable enzyme derived from the archael bacterium AEPII 1a. In one aspect, the enzyme has a molecular weight of about 60.9 kilodaltons and has a cellulase activity. The enzyme can be produced from native or recombinant host cells, and can be used to aid in the digestion of cellulose. The invention also provides polypeptides having endoglucanase activity having homology to SEQ ID NO:2.

Abstract translation: 一方面，本发明提供了源自阿尔茨海默氏细菌AEPII1a的纯化的热稳定性酶。 一方面，该酶具有约60.9千道尔顿的分子量，并具有纤维素酶活性。 该酶可以由天然的或重组的宿主细胞产生，并可用于帮助消化纤维素。 本发明还提供具有与SEQ ID NO：2具有同源性的内切葡聚糖酶活性的多肽。

公开(公告)号：US20080279985A1

公开(公告)日：2008-11-13

申请号：US11840149

申请日：2007-08-16

Applicant: Jay M. Short ,  Edward Bylina ,  Ronald V. Swanson ,  Eric J. Mathur ,  David E. Lam

Inventor： Jay M. Short ,  Edward Bylina ,  Ronald V. Swanson ,  Eric J. Mathur ,  David E. Lam

IPC: A23L1/30 ,  C12N9/44 ,  C12P21/04 ,  C12P19/02 ,  A62D3/02 ,  G06F19/00 ,  C09K8/60 ,  C10L1/10 ,  C40B50/06

CPC classification number: C12N9/2468 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2454 ,  C12N9/2457 ,  C12N9/2465 ,  C12N9/2494 ,  C12Y302/01004 ,  C12Y302/01022 ,  C12Y302/01025 ,  C12Y302/01041 ,  C12Y302/01078

Abstract: The invention relates to a polypeptide having glycosidase activity. In addition methods of designing new glycosidases and method of use thereof are also provided. The glycosidases have increased activity and stability at increased pH and Temperature.

Abstract translation: 本发明涉及具有糖苷酶活性的多肽。 此外，还提供了设计新的糖苷酶的方法及其使用方法。 糖苷酶在增加的pH和温度下具有增加的活性和稳定性。

公开(公告)号：US07422876B2

公开(公告)日：2008-09-09

申请号：US11034411

申请日：2005-01-11

Applicant: Jay M. Short ,  Eric J. Mathur ,  David E. Lam

Inventor： Jay M. Short ,  Eric J. Mathur ,  David E. Lam

IPC: C12P21/06 ,  C07H21/04 ,  C07K14/00

CPC classification number: C07H21/00 ,  C12N9/2437 ,  C12N15/1027 ,  C12Y302/01004 ,  G06F19/22

Abstract: The invention relates to carboxymethyl cellulases and to polynucleotides encoding the carboxymethyl cellulases. In addition methods of designing new carboxymethyl cellulases and method of use thereof are also provided. The carboxymethyl cellulases have increased activity and stability at increased pH and temperature.

Abstract translation: 本发明涉及羧甲基纤维素酶和编码羧甲基纤维素酶的多核苷酸。 此外，还提供了设计新的羧甲基纤维素酶的方法及其使用方法。 羧甲基纤维素酶在增加的pH和温度下具有增加的活性和稳定性。

公开(公告)号：US07049101B1

公开(公告)日：2006-05-23

申请号：US09656309

申请日：2000-09-06

Applicant: Walter Callen ,  Eric J. Mathur

Inventor： Walter Callen ,  Eric J. Mathur

IPC: C12P19/34 ,  C12N9/12

CPC classification number: C12N9/1252 ,  A01K2217/05

Abstract: The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90° C. up to 113° C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of designing new thermostable DNA polymerases and methods of use thereof are also provided. The polymerases have increased activity and stability at increased pH and temperature.

公开(公告)号：US6074867A

公开(公告)日：2000-06-13

申请号：US951086

申请日：1997-10-15

Applicant: David E. Lam ,  Eric J. Mathur

Inventor： David E. Lam ,  Eric J. Mathur

IPC: C12N15/09 ,  C07K16/40 ,  C12N1/21 ,  C12N9/42 ,  C12P19/14 ,  C12R1/19 ,  C12S3/02 ,  D21C1/00 ,  D06M16/00 ,  C11D3/000

CPC classification number: C12N9/2437 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01021

Abstract: A purified thermostable enzyme is derived from the archael bacterium AEPII1A. The enzyme has a molecular weight of about 60.9 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of cellulose where desired.

Abstract translation: 纯化的热稳定酶源自阿尔茨海默氏菌AEPII1A。 该酶的分子量约为60.9千道尔顿，具有纤维素酶活性。 该酶可以由天然的或重组的宿主细胞产生，并且可以用于有助于纤维素的消化。

公开(公告)号：US5962258A

公开(公告)日：1999-10-05

申请号：US518615

申请日：1995-08-23

Applicant: Eric J. Mathur ,  David E. Lam

Inventor： Eric J. Mathur ,  David E. Lam

IPC: C07H21/00 ,  C12N15/56 ,  C12P21/02 ,  C07H21/04 ,  C12N15/63

CPC classification number: C07H21/00

Abstract: A purified thermostable enzyme is derived from the eubacterium T. maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 35 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of cellulose where desired.

Abstract translation: 纯化的热稳定性酶源自海马杆菌。 该酶具有通过约35千道尔顿的凝胶电泳测定的分子量，并且具有纤维素酶活性。 该酶可以由天然的或重组的宿主细胞产生，并且可以用于有助于纤维素的消化。

公开(公告)号：US5545552A

公开(公告)日：1996-08-13

申请号：US424921

申请日：1995-04-19

Applicant: Eric J. Mathur

Inventor： Eric J. Mathur

IPC: C12N9/12 ,  C12N9/22 ,  C12N15/54 ,  C12P19/34 ,  C12Q1/68 ,  C12N1/21 ,  C12N15/63

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: Purified thermostable Pyrococcus furiosus DNA polymerase that migrates on a non-denaturing polyacrylamide gel faster than phosphorylase B and Taq polymerase and more slowly than bovine serum albumin and has an estimated molecular weight of 90,000-93,000 daltons when compared with a Taq polymerase standard assigned a molecular weight of 94,000 daltons.

公开(公告)号：US09556413B2

公开(公告)日：2017-01-31

申请号：US12532403

申请日：2008-03-21

Applicant: J. Craig Venter ,  Eric J. Mathur ,  Gerardo Vicente Toledo ,  Hwai Wen Chang ,  Wayne A. Green

Inventor： J. Craig Venter ,  Eric J. Mathur ,  Gerardo Vicente Toledo ,  Hwai Wen Chang ,  Wayne A. Green

IPC: C12M1/34 ,  C12N1/20 ,  C12M3/00 ,  C12M1/00

CPC classification number: C12N1/20 ,  C12M23/44 ,  C12M45/03 ,  C12M47/04

Abstract: The invention described below relates to an enclosed cell sorting device and methods of using the device. The device is constructed so that the entire process of cell sorting can be conducted under fully anaerobic conditions to retain viability of anaerobic cells before, during, and after cell sorting. This is accomplished by creating an anaerobic atmosphere for the high speed cell sorter and all its components and by the use of airlocks that allow the introduction of anaerobic containers into the chamber containing the sample.

Abstract translation: 下面描述的本发明涉及封闭的细胞分选装置和使用该装置的方法。 构建该装置使得整个细胞分选过程可以在完全厌氧条件下进行，以在细胞分选之前，期间和之后保留厌氧细胞的活力。 这是通过为高速细胞分选器及其所有组分产生厌氧气氛以及通过使用允许将厌氧容器引入含有样品的室的气闸实现的。