Fluorescence-quenching used to detect nucleic acid oligomer hybrization events at high salt concentrations
    2.
    发明申请
    Fluorescence-quenching used to detect nucleic acid oligomer hybrization events at high salt concentrations 审中-公开
    荧光猝灭用于检测高盐浓度下的核酸低聚物杂化事件

    公开(公告)号:US20060188877A1

    公开(公告)日:2006-08-24

    申请号:US10529472

    申请日:2002-11-08

    IPC分类号: C12Q1/68

    摘要: A method is described for detecting nucleic acid oligomer hybridization events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified nucleic acid oligomers 201, wherein said nucleic acid oligomers 201 are modified by at least one type of fluorophore 102 bound to it. The further steps of the inventive method are: providing a sample that includes nucleic acid oligomers, contacting said sample with the modified surface, adjusting a defined salt concentration of greater than 0.5 mol/l in the solution surrounding the modified nucleic acid oligomers, detecting the fluorescence of the fluorophore and comparing the detected fluorescence intensity with reference values.

    摘要翻译: 描述了通过荧光猝灭检测核酸低聚物杂交事件的方法,其包括提供改性表面的第一步骤。 表面的修饰在于至少一种类型的修饰的核酸低聚物201的结合,其中所述核酸寡聚物201被与其结合的至少一种类型的荧光团102修饰。 本发明方法的进一步步骤是:提供包含核酸低聚物的样品,使所述样品与修饰表面接触,调节在修饰的核酸寡聚物周围的溶液中大于0.5mol / l的限定的盐浓度,检测 荧光团的荧光,并将检测到的荧光强度与参考值进行比较。