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公开(公告)号：US20120219029A1
公开(公告)日：2012-08-30
申请号：US13342471
申请日：2012-01-03
CPC分类号： G01J3/4406 , G01J3/021 , G01J3/10 , G01N21/6428 , G01N21/6458 , G01N2021/6419 , G01N2021/6441
摘要： The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.
摘要翻译： 本发明提供了一种称为脉冲 - 多重激发或PME的技术。 该技术提供了一种新的荧光检测方法，可应用于信息性SNP的高通量鉴定，这可以导致对遗传疾病的更准确的诊断，更好的预后风险易感性或散发性突变的鉴定。 PME技术有两个显着增加荧光灵敏度的主要优点：（1）基因组测定中所有荧光团的最佳激发和（2）“色盲”检测，其比标准波长分辨检测收集明显更多的光。 PME技术的成功实施将广泛应用于临床诊断，取证和一般测序方法中的常规应用，并将为大多数人群提供靶向序列变异测定的能力，灵活性和可移植性。
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公开(公告)号：US06995841B2
公开(公告)日：2006-02-07
申请号：US09941165
申请日：2001-08-28
CPC分类号： G01J3/4406 , G01J3/021 , G01J3/10 , G01N21/6428 , G01N21/6458 , G01N2021/6419 , G01N2021/6441
摘要： The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.
摘要翻译： 本发明提供了一种称为脉冲 - 多重激发或PME的技术。 该技术提供了一种新的荧光检测方法，可应用于信息性SNP的高通量鉴定，这可以导致对遗传疾病的更准确的诊断，更好的预后风险易感性或散发性突变的鉴定。 PME技术有两个显着增加荧光灵敏度的主要优点：（1）基因组测定中所有荧光团的最佳激发和（2）“色盲”检测，其比标准波长分辨检测收集明显更多的光。 PME技术的成功实施将广泛应用于临床诊断，取证和一般测序方法中的常规应用，并将为大多数人群提供靶向序列变异测定的能力，灵活性和可移植性。
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公开(公告)号：US08089628B2
公开(公告)日：2012-01-03
申请号：US12388358
申请日：2009-02-18
IPC分类号： G01N21/25
CPC分类号： G01J3/4406 , G01J3/021 , G01J3/10 , G01N21/6428 , G01N21/6458 , G01N2021/6419 , G01N2021/6441
摘要： The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.
摘要翻译： 本发明提供了一种称为脉冲 - 多重激发或PME的技术。 该技术提供了一种新的荧光检测方法，可应用于信息性SNP的高通量鉴定，这可以导致对遗传疾病的更准确的诊断，更好的预后风险易感性或散发性突变的鉴定。 PME技术有两个显着增加荧光灵敏度的主要优点：（1）基因组测定中所有荧光团的最佳激发和（2）“色盲”检测，其比标准波长分辨检测收集明显更多的光。 PME技术的成功实施将广泛应用于临床诊断，取证和一般测序方法中的常规应用，并将为大多数人群提供靶向序列变异测定的能力，灵活性和可移植性。
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公开(公告)号：US07511811B2
公开(公告)日：2009-03-31
申请号：US11248910
申请日：2005-10-12
IPC分类号： G01J3/30
CPC分类号： G01J3/4406 , G01J3/021 , G01J3/10 , G01N21/6428 , G01N21/6458 , G01N2021/6419 , G01N2021/6441
摘要： The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. This technology differs significantly from the current state-of-the-art DNA sequencing instrumentation, which features single source excitation and color dispersion for DNA sequence identification. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.
摘要翻译： 本发明提供了一种称为脉冲 - 多重激发或PME的技术。 该技术提供了一种新的荧光检测方法，可应用于信息性SNP的高通量鉴定，这可以导致对遗传疾病的更准确的诊断，更好的预后风险易感性或散发性突变的鉴定。 PME技术有两个显着增加荧光灵敏度的主要优点：（1）基因组测定中所有荧光团的最佳激发和（2）“色盲”检测，其比标准波长分辨检测收集明显更多的光。 该技术与目前最先进的DNA测序仪器显着不同，其具有用于DNA序列鉴定的单源激发和色散。 PME技术的成功实施将广泛应用于临床诊断，取证和一般测序方法中的常规应用，并将为大多数人群提供靶向序列变异测定的能力，灵活性和可移植性。
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公开(公告)号：US20090156429A1
公开(公告)日：2009-06-18
申请号：US12388358
申请日：2009-02-18
CPC分类号： G01J3/4406 , G01J3/021 , G01J3/10 , G01N21/6428 , G01N21/6458 , G01N2021/6419 , G01N2021/6441
摘要： The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.
摘要翻译： 本发明提供了一种称为脉冲 - 多重激发或PME的技术。 该技术提供了一种新的荧光检测方法，可应用于信息性SNP的高通量鉴定，这可以导致对遗传疾病的更准确的诊断，更好的预后风险易感性或散发性突变的鉴定。 PME技术有两个显着增加荧光灵敏度的主要优点：（1）基因组测定中所有荧光团的最佳激发和（2）“色盲”检测，其比标准波长分辨检测收集明显更多的光。 PME技术的成功实施将广泛应用于临床诊断，取证和一般测序方法中的常规应用，并将为大多数人群提供靶向序列变异测定的能力，灵活性和可移植性。
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6.发明授权Piezo activated mode tracking system for widely tunable mode-hop-free external cavity mid-IR semiconductor lasers 失效压电激活模式跟踪系统，用于广泛的无模式跳跃型外腔中红外半导体激光器公开(公告)号：US07733924B2
公开(公告)日：2010-06-08
申请号：US11203785
申请日：2005-08-15
申请人： Gerard Wysocki , Frank K. Tittel , Robert F. Curl
发明人： Gerard Wysocki , Frank K. Tittel , Robert F. Curl
IPC分类号： H01S3/10
CPC分类号： H01S5/141 , B82Y20/00 , G01N21/3504 , G01N21/39 , H01S3/139 , H01S5/0222 , H01S5/02415 , H01S5/0617 , H01S5/06817 , H01S5/1039 , H01S5/3401
摘要： A widely tunable, mode-hop-free semiconductor laser operating in the mid-IR comprises a QCL laser chip having an effective QCL cavity length, a diffraction grating defining a grating angle and an external cavity length with respect to said chip, and means for controlling the QCL cavity length, the external cavity length, and the grating angle. The laser of claim 1 wherein said chip may be tuned over a range of frequencies even in the absence of an anti-reflective coating. The diffraction grating is controllably pivotable and translatable relative to said chip and the effective QCL cavity length can be adjusted by varying the injection current to the chip. The laser can be used for high resolution spectroscopic applications and multi species trace-gas detection. Mode-hopping is avoided by controlling the effective QCL cavity length, the external cavity length, and the grating angle so as to replicate a virtual pivot point.
摘要翻译： 在中红外工作的可广泛调谐的无模式跳变半导体激光器包括具有有效QCL腔长度的QCL激光器芯片，相对于所述芯片限定光栅角度和外部腔体长度的衍射光栅，以及用于 控制QCL腔长度，外腔长度和光栅角度。 2.如权利要求1所述的激光器，其特征在于，即使在没有抗反射涂层的情况下，也可以在频率范围内调节所述芯片。 衍射光栅相对于所述芯片可控制地枢转和平移，并且可以通过改变对芯片的注入电流来调节有效的QCL腔体长度。 激光可用于高分辨率光谱应用和多种痕量气体检测。 通过控制有效的QCL腔长度，外腔长度和光栅角度来避免跳频，以便复制虚拟枢轴点。
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公开(公告)号：US20090303476A1
公开(公告)日：2009-12-10
申请号：US12089692
申请日：2006-11-09
IPC分类号： G01J3/433
CPC分类号： G01N21/1702 , G01N21/276 , G01N21/39 , G01N2021/1704
摘要： Novel methods and laser spectroscopic systems for accurately measuring the concentration of compounds are disclosed herein. The disclosed methods utilize a modulation cancellation technique resulting in a significantly increase in the sensitivity and accuracy of laser spectroscopic measurements. In general, the methods and systems utilize modulation phase-shifting and amplitude attenuation to cancel the signals detected from at least two modulated light beams. Thus, any signal detected will be directly proportional to the concentration measurement.
摘要翻译： 本文公开了用于精确测量化合物浓度的新方法和激光光谱系统。 所公开的方法利用调制消除技术，导致激光光谱测量的灵敏度和精度的显着增加。 通常，方法和系统利用调制相移和幅度衰减来消除从至少两个调制光束检测到的信号。 因此，检测到的任何信号将与浓度测量成正比。
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公开(公告)号：US08009293B2
公开(公告)日：2011-08-30
申请号：US12089692
申请日：2006-11-09
IPC分类号： G01N21/00
CPC分类号： G01N21/1702 , G01N21/276 , G01N21/39 , G01N2021/1704
摘要： Novel methods and laser spectroscopic systems for accurately measuring the concentration of compounds are disclosed herein. The disclosed methods utilize a modulation cancellation technique resulting in a significantly increase in the sensitivity and accuracy of laser spectroscopic measurements. In general, the methods and systems utilize modulation phase-shifting and amplitude attenuation to cancel the signals detected from at least two modulated light beams. Thus, any signal detected will be directly proportional to the concentration measurement.
摘要翻译： 本文公开了用于精确测量化合物浓度的新方法和激光光谱系统。 所公开的方法利用调制消除技术，导致激光光谱测量的灵敏度和精度的显着增加。 通常，方法和系统利用调制相移和幅度衰减来消除从至少两个调制光束检测到的信号。 因此，检测到的任何信号将与浓度测量成正比。
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