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223 条记录 (耗时 0.029 秒)

    Systematic evolution of ligands by exponential enrichment: blended SELEX
    1.
    发明授权
    Systematic evolution of ligands by exponential enrichment: blended SELEX 失效
    配体通过指数富集系统演化：混合SELEX

    公开(公告)号：US07176295B2

    公开(公告)日：2007-02-13

    申请号：US10263456

    申请日：2002-10-02

    申请人： Gregory Biesecker ,  Sumeda Jayasena ,  Larry Gold ,  Drew Smith ,  Gary P. Kirschenheuter

    发明人： Gregory Biesecker ,  Sumeda Jayasena ,  Larry Gold ,  Drew Smith ,  Gary P. Kirschenheuter

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02

    CPC分类号： C12P19/34 ,  A61K47/64 ,  C07K5/1008 ,  C07K19/00 ,  C12N15/1048

    摘要： A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (SEQ ID NO:2). This elastase blended nucleic acid ligand inhibits the biological activity of elastase.

    摘要翻译： 描述了一种用于产生含有非核酸功能单元的混合核酸配体的方法。 具体地，将SELEX鉴定的整合素gpIIbIIIa的RNA配体与肽Gly-Arg-Gly-Asp-Thr-Pro（SEQ ID NO：1）缀合。 这种混合RNA配体以高特异性抑制gpIIbIIIa的生物活性。 还描述了与N-甲氧基琥珀酰基-Ala-Ala-Pro-Val-氯甲基酮（SEQ ID NO：2）偶联的弹性蛋白酶的单链DNA配体。 该弹性蛋白酶共混核酸配体抑制弹性蛋白酶的生物活性。

    Lung cancer biomarkers and uses thereof
    8.
    发明授权
    Lung cancer biomarkers and uses thereof 有权

    公开(公告)号：US10359425B2

    公开(公告)日：2019-07-23

    申请号：US12556480

    申请日：2009-09-09

    申请人： Larry Gold ,  Marty Stanton ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A. E. Stewart

    发明人： Larry Gold ,  Marty Stanton ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A. E. Stewart

    IPC分类号： G01N33/574

    摘要： The present application includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of lung cancer. In one aspect, the application provides biomarkers that can be used alone or in various combinations to diagnose lung cancer or permit the differential diagnosis of pulmonary nodules as benign or malignant. In another aspect, methods are provided for diagnosing lung cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 1, Col. 2, wherein the individual is classified as having lung cancer, or the likelihood of the individual having lung cancer is determined, based on the at least one biomarker value.

    Method for purification and identification of sperm cells
    9.
    发明授权
    Method for purification and identification of sperm cells 有权
    纯化和鉴定精子细胞的方法

    公开(公告)号：US08703416B2

    公开(公告)日：2014-04-22

    申请号：US13525271

    申请日：2012-06-15

    申请人： Glenn Sanders ,  Evaldas Katilius ,  Larry Gold

    发明人： Glenn Sanders ,  Evaldas Katilius ,  Larry Gold

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12N15/115 ,  C12N5/061 ,  C12N2310/16 ,  C12N2320/10 ,  C12N2330/00

    摘要： The present disclosure describes the isolation, identification and purification of aptamers having sufficiently high affinity and specificity to capture and immobilize intact sperm cells in the presence of female epithelial cells and other non-sperm semen components. The present disclosure also describes affinity-based methods for the detection of sperm cells in samples, including from forensic sample surrogates consisting of swab eluates containing a mixture of HeLa cells and sperm cells. The present disclosure describes methods for eluting sperm cell samples from swabs; methods for purifying sperm cells and methods for amplification and analysis of male DNA. The affinity-based system described herein is inexpensive, simple to use and easily implemented in forensic laboratories.

    摘要翻译： 本公开描述了具有足够高的亲和性和特异性的适体的分离，鉴定和纯化，以在存在雌性上皮细胞和其它非精液精液组分的情况下捕获和固定完整精子细胞。 本公开还描述了用于检测样品中精子细胞的基于亲和性的方法，包括由包含HeLa细胞和精子细胞的混合物的拭子洗脱液组成的法医样品替代物。 本公开描述了从拭子洗脱精子细胞样品的方法; 纯化精子细胞的方法和扩增和分析雄性DNA的方法。 本文描述的基于亲和性的系统是廉价的，易于使用并且容易地在法医实验室中实施。