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公开(公告)号：US08685890B2
公开(公告)日：2014-04-01
申请号：US13147289
申请日：2010-02-04
申请人： Gregory Paul Winter , Christian Heinis , Elise Bernard , David Loakes , John Tite , Marina Vaysburd , Daniel Paul Teufel , Lutz Riechmann
发明人： Gregory Paul Winter , Christian Heinis , Elise Bernard , David Loakes , John Tite , Marina Vaysburd , Daniel Paul Teufel , Lutz Riechmann
IPC分类号： C40B30/04
CPC分类号： C12N15/1037 , A61K47/55 , A61K47/64 , A61K47/643 , C07K14/001 , C12N15/1044 , C12N15/1058 , C40B40/08 , C40B40/10 , C40B50/06 , G01N33/531
摘要： The invention relates to a method for providing a multispecific peptide ligand comprising a polypeptide covalently linked to a molecular scaffold at three or more amino acid residues and capable of binding to two or more separate targets, comprising the steps of: (a) providing a first repertoire of polypeptides, each polypeptide comprising two or more reactive groups capable of covalent linkage to a molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; (b) providing a second repertoire of polypeptides as described in (a); (c) joining at least one loop of one or more members of the first repertoire to at least one loop of one or more members of the second repertoire to form at least one polypeptide comprising two loops, and (d) conjugating the composite polypeptide(s) to a molecular scaffold at at least three amino acid positions.
摘要翻译： 本发明涉及一种提供多特异性肽配体的方法，所述多特异性肽配体包含在三个或更多个氨基酸残基处共价连接到分子支架并能够结合两个或更多个单独靶标的多肽，包括以下步骤：（a）提供第一 多肽的所有组成部分，每个多肽包含两个或更多个能够与分子支架共价连接的反应性基团，以及至少一个环，其包含两个或更多个氨基酸的序列，对应于两个所述反应性基团; （b）提供如（a）所述的第二组多肽; （c）将第一组合物中的一个或多个成员的至少一个环连接到第二组合物的一个或多个成员的至少一个环，以形成至少一个包含两个环的多肽，和（d）将复合多肽（ s）至少在三个氨基酸位置分子支架。
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公开(公告)号：US20120101256A1
公开(公告)日：2012-04-26
申请号：US13147289
申请日：2010-02-04
申请人： Gregory Paul Winter , Christian Heinis , Elise Bernard , David Loakes , John Tite , Marina Vaysburd , Daniel Paul Teufel , Lutz Riechmann
发明人： Gregory Paul Winter , Christian Heinis , Elise Bernard , David Loakes , John Tite , Marina Vaysburd , Daniel Paul Teufel , Lutz Riechmann
CPC分类号： C12N15/1037 , A61K47/55 , A61K47/64 , A61K47/643 , C07K14/001 , C12N15/1044 , C12N15/1058 , C40B40/08 , C40B40/10 , C40B50/06 , G01N33/531
摘要： The invention relates to a method for providing a multispecific peptide ligand comprising a polypeptide covalently linked to a molecular scaffold at three or more amino acid residues and capable of binding to two or more separate targets, comprising the steps of: (a) providing a first repertoire of polypeptides, each polypeptide comprising two or more reactive groups capable of covalent linkage to a molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; (b) providing a second repertoire of polypeptides as described in (a); (c) joining at least one loop of one or more members of the first repertoire to at least one loop of one or more members of the second repertoire to form at least one polypeptide comprising two loops, and (d) conjugating the composite polypeptide(s) to a molecular scaffold at at least three amino acid positions.
摘要翻译： 本发明涉及一种提供多特异性肽配体的方法，所述多特异性肽配体包含在三个或更多个氨基酸残基处共价连接到分子支架并能够结合两个或更多个单独靶标的多肽，包括以下步骤：（a）提供第一 多肽的所有组成部分，每个多肽包含两个或更多个能够与分子支架共价连接的反应性基团，以及至少一个环，其包含两个或更多个氨基酸的序列，对应于两个所述反应性基团; （b）提供如（a）所述的第二组多肽; （c）将第一组合物中的一个或多个成员的至少一个环连接到第二组合物的一个或多个成员的至少一个环，以形成至少一个包含两个环的多肽，和（d）将复合多肽（ s）至少在三个氨基酸位置分子支架。
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公开(公告)号：US08778844B2
公开(公告)日：2014-07-15
申请号：US13147257
申请日：2010-02-04
IPC分类号： C40B30/04
CPC分类号： C12N15/1037 , A61K47/55 , A61K47/64 , A61K47/643 , C07K14/001 , C12N15/1044 , C12N15/1058 , C40B40/08 , C40B40/10 , C40B50/06 , G01N33/531
摘要： The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.
摘要翻译： 本发明涉及一种修饰一种或多种肽配体的方法，其包括在两个或更多个氨基酸残基处与分子支架共价连接的多肽，包括提供一个或多个肽配体的步骤，其中所述多肽包含两个或多个反应性基团 其形成与分子支架的共价键，以及至少一个环，其包含在两个所述反应性基团之间对位的两个或更多个氨基酸的序列; 将肽配体暴露于一种或多种蛋白酶; 并根据蛋白水解切割的程度对配体进行分选。
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公开(公告)号：US20120142541A1
公开(公告)日：2012-06-07
申请号：US13147257
申请日：2010-02-04
CPC分类号： C12N15/1037 , A61K47/55 , A61K47/64 , A61K47/643 , C07K14/001 , C12N15/1044 , C12N15/1058 , C40B40/08 , C40B40/10 , C40B50/06 , G01N33/531
摘要： The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.
摘要翻译： 本发明涉及一种修饰一种或多种肽配体的方法，其包括在两个或更多个氨基酸残基处与分子支架共价连接的多肽，包括提供一个或多个肽配体的步骤，其中所述多肽包含两个或多个反应性基团 其形成与分子支架的共价键，以及至少一个环，其包含在两个所述反应性基团之间对位的两个或更多个氨基酸的序列; 将肽配体暴露于一种或多种蛋白酶; 并根据蛋白水解切割的程度对配体进行分选。
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公开(公告)号：US09518081B2
公开(公告)日：2016-12-13
申请号：US13390252
申请日：2010-08-12
CPC分类号： C12N15/1037 , C07K1/00 , C07K1/042 , C07K1/113 , C07K2318/00
摘要： The invention relates to a method for altering the conformational diversity of a first repertoire of polypeptide ligands, comprising a plurality of polypeptides comprising at least two reactive groups separated by a loop sequence covalently linked to a molecular scaffold which forms covalent bonds with said reactive groups, to produce a second repertoire of polypeptide ligands, comprising assembling said second repertoire from the polypeptides and structural scaffold of said first repertoire, incorporating one of the following alterations: (a) altering at least one reactive group; or (b) altering the nature of the molecular scaffold; or (c) altering the bond between at least one reactive group and the molecular scaffold; or (d) any combination of (a), (b) or (c).
摘要翻译： 本发明涉及一种用于改变多肽配体的第一组分的构象多样性的方法，其包含多个多肽，所述多肽包含至少两个反应性基团，所述反应基团通过共价连接到与所述反应性基团形成共价键的分子支架的环序列分开， 以产生多肽配体的第二组成成分，其包括从所述第一组合物的多肽和结构性支架组装所述第二组分，并结合以下改变之一：（a）改变至少一个反应性基团; 或（b）改变分子支架的性质; 或（c）改变至少一个反应性基团和分子支架之间的键; 或（d）（a），（b）或（c）的任何组合。
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公开(公告)号：US20120172235A1
公开(公告)日：2012-07-05
申请号：US13390252
申请日：2010-08-12
CPC分类号： C12N15/1037 , C07K1/00 , C07K1/042 , C07K1/113 , C07K2318/00
摘要： The invention relates to a method for altering the conformational diversity of a first repertoire of polypeptide ligands, comprising a plurality of polypeptides comprising at least two reactive groups separated by a loop sequence covalently linked to a molecular scaffold which forms covalent bonds with said reactive groups, to produce a second repertoire of polypeptide ligands, comprising assembling said second repertoire from the polypeptides and structural scaffold of said first repertoire, incorporating one of the following alterations: (a) altering at least one reactive group; or (b) altering the nature of the molecular scaffold; or (c) altering the bond between at least one reactive group and the molecular scaffold; or (d) any combination of (a), (b) or (c).
摘要翻译： 本发明涉及一种用于改变多肽配体的第一组分的构象多样性的方法，其包含多个多肽，所述多肽包含至少两个反应性基团，所述反应基团通过共价连接到与所述反应性基团形成共价键的分子支架的环序列分开， 以产生多肽配体的第二组成成分，其包括从所述第一组合物的多肽和结构性支架组装所述第二组分，并结合以下改变之一：（a）改变至少一个反应性基团; 或（b）改变分子支架的性质; 或（c）改变至少一个反应性基团和分子支架之间的键; 或（d）（a），（b）或（c）的任何组合。
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公开(公告)号：US07442159B1
公开(公告)日：2008-10-28
申请号：US09710444
申请日：2000-11-10
CPC分类号： C40B40/02 , C07K2319/02 , C07K2319/41 , C07K2319/43 , C07K2319/50 , C07K2319/70 , C07K2319/735 , C12N15/1037 , C12N15/62
摘要： The present invention concerns a method for the selection of a virus comprising the steps of: (a) providing a virus encoding and displaying a fusion polypeptide, said fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a viral coat protein polypeptide, wherein said virus comprises a cleavable site located within a displayed polypeptide; (b) exposing the virus to a cleaving agent; (c) propagating the virus comprising intact fusion protein.
摘要翻译： 本发明涉及一种选择病毒的方法，包括以下步骤：（a）提供编码和显示融合多肽的病毒，所述融合多肽包含插入到病毒外壳蛋白多肽序列中的异源多肽，其中所述 病毒包含位于显示的多肽内的可切割位点; （b）将病毒暴露于裂解剂; （c）扩增包含完整融合蛋白的病毒。
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公开(公告)号：US5965405A
公开(公告)日：1999-10-12
申请号：US165530
申请日：1993-12-13
申请人： Gregory Paul Winter , Lutz Riechmann , Geoffrey Thomas Yarranton , Mark William Bodmer , Raymond John Owens
发明人： Gregory Paul Winter , Lutz Riechmann , Geoffrey Thomas Yarranton , Mark William Bodmer , Raymond John Owens
CPC分类号： C07K16/30 , C07K16/40 , C07K2317/24 , C07K2317/56 , C07K2319/00
摘要： Methods for preparing Fv fragments which lack linking polypeptides in eukaryotic cells are provided.
摘要翻译： 提供了在真核细胞中缺乏连接多肽的Fv片段的制备方法。
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公开(公告)号：US20120101253A1
公开(公告)日：2012-04-26
申请号：US13147308
申请日：2010-02-04
IPC分类号： C07K7/64
CPC分类号： C12N15/1037 , A61K47/55 , A61K47/64 , A61K47/643 , C07K14/001 , C12N15/1044 , C12N15/1058 , C40B40/08 , C40B40/10 , C40B50/06 , G01N33/531
摘要： The invention relates to a peptide ligand comprising a polypeptide linked to a molecular scaffold at n attachment points, wherein said polypeptide is cyclised and forms n separate loops subtended between said n attachment points on the molecular scaffold, wherein n is greater than or equal to 2.
摘要翻译： 本发明涉及一种肽配体，其包含在n个连接点处与分子支架连接的多肽，其中所述多肽被环化并形成对分子支架上的所述n个附着点之间的n个分开的环，其中n大于或等于2 。
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公开(公告)号：US08236931B2
公开(公告)日：2012-08-07
申请号：US12447908
申请日：2007-10-26
CPC分类号： C07K16/22 , C07K16/241 , C07K16/42 , C07K2317/31 , C07K2317/567 , C07K2317/569
摘要： An inhibitor of the aggregation of immunoglobulin chains is provided. The inhibitor may comprise or consist of a polypeptide which comprises or consists of (a) an amino acid sequence corresponding to the amino acid sequence of the FR1 region of an immunoglobulin light chain variable domain, or part thereof which includes amino acid residue 12, (b) an amino acid sequence corresponding to the amino acid sequence of the immunoglobulin-binding domain of bacterial superantigen Protein L, or part thereof, and/or (c) an amino acid sequence corresponding to the amino acid sequence of the immunoglobulin-binding domain of streptococcal protein G, or part thereof, or a variant, fusion or derivative thereof, or a fusion of a variant or derivative thereof which retains the ability of the parent polypeptide to inhibit aggregation of immunoglobulin chains, or domains thereof. Other versions of the inhibitor are also provided.
摘要翻译： 提供免疫球蛋白链聚集的抑制剂。 抑制剂可以包含或由其组成，其包含（a）对应于免疫球蛋白轻链可变结构域的FR1区的氨基酸序列的氨基酸序列或其部分，其包括氨基酸残基12（ b）对应于细菌超抗原蛋白L或其部分的免疫球蛋白结合结构域的氨基酸序列的氨基酸序列和/或（c）对应于免疫球蛋白结合结构域的氨基酸序列的氨基酸序列 的链球菌蛋白G或其部分，或其变体，融合物或衍生物，或其保留母体多肽抑制免疫球蛋白链的聚集或其结构域的能力的变体或衍生物的融合物。 还提供其他版本的抑制剂。
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