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1.发明授权Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same 有权用于获得热稳定酶的方法，具有新催化活性的水生栖热菌的DNA聚合酶I变体，获得其的方法及其应用公开(公告)号：US07417133B2
公开(公告)日：2008-08-26
申请号：US10787219
申请日：2004-02-27
IPC分类号： C07H21/04
CPC分类号： C07K14/43563 , A61K38/00 , C12N9/1252 , Y02A50/473
摘要： The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.
摘要翻译： 本发明提供一种获得耐热性酶的方法。 本发明还提供了水生栖热菌的DNA聚合酶I的变体。 本发明还提供鉴定具有增强的催化活性的突变型DNA聚合酶的方法。 本发明还提供了编码突变型DNA聚合酶的多核苷酸，表达系统和宿主细胞。 此外，本发明提供了进行逆转录聚合酶链反应（RT-PCR）和试剂盒以促进其的方法。
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2.发明授权Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same 有权用于获得热稳定酶的方法，具有新催化活性的水生栖热菌的DNA聚合酶I变体，获得其的方法及其应用公开(公告)号：US07488816B2
公开(公告)日：2009-02-10
申请号：US11065943
申请日：2005-02-25
IPC分类号： C07H21/04
CPC分类号： C07K14/43563 , A61K38/00 , C12N9/1252 , Y02A50/473
摘要： The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.
摘要翻译： 本发明提供一种获得耐热性酶的方法。 本发明还提供了水生栖热菌的DNA聚合酶I的变体。 本发明还提供鉴定具有增强的催化活性的突变型DNA聚合酶的方法。 本发明还提供了编码突变型DNA聚合酶的多核苷酸，表达系统和宿主细胞。 此外，本发明提供了进行逆转录聚合酶链反应（RT-PCR）和试剂盒以促进其的方法。
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公开(公告)号：US07442159B1
公开(公告)日：2008-10-28
申请号：US09710444
申请日：2000-11-10
CPC分类号： C40B40/02 , C07K2319/02 , C07K2319/41 , C07K2319/43 , C07K2319/50 , C07K2319/70 , C07K2319/735 , C12N15/1037 , C12N15/62
摘要： The present invention concerns a method for the selection of a virus comprising the steps of: (a) providing a virus encoding and displaying a fusion polypeptide, said fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a viral coat protein polypeptide, wherein said virus comprises a cleavable site located within a displayed polypeptide; (b) exposing the virus to a cleaving agent; (c) propagating the virus comprising intact fusion protein.
摘要翻译： 本发明涉及一种选择病毒的方法，包括以下步骤：（a）提供编码和显示融合多肽的病毒，所述融合多肽包含插入到病毒外壳蛋白多肽序列中的异源多肽，其中所述 病毒包含位于显示的多肽内的可切割位点; （b）将病毒暴露于裂解剂; （c）扩增包含完整融合蛋白的病毒。
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4.发明申请Methods for Obtaining Thermostable Enzymes, Dna Polymerase I Variants from Thermus Aquaticus Having New Catalytic Activities, Methods for Obtaining the Same, and Applications to the Same 有权获得热稳定酶的方法，具有新的催化活性的水生栖热菌的Dna聚合酶I变体，获得其的方法及其应用公开(公告)号：US20080014609A1
公开(公告)日：2008-01-17
申请号：US10590810
申请日：2005-02-25
CPC分类号： C07K14/43563 , A61K38/00 , C12N9/1252 , Y02A50/473
摘要： A thermostable mutant DNA polymerase; a method for obtaining the thernmostable mutant DNA polymerase by identifying a thermostable mutant polypeptide exhibiting enzymatic activity, wherein the thermostable mutant polypeptide is a variant of DNA polymerase I obtained from Thermus aquaticus; a polynucleotide, an expression vector, and a host cell encoding the thermostable mutant DNA polymerase; and a method of performing a reverse transcription polymerase chain reaction (RT-PCR) utilizing the thermostable mutant DNA polymerase, as well as a kit for facilitating the same.
摘要翻译： 热稳定突变体DNA聚合酶; 通过鉴定表现出酶活性的热稳定突变体多肽来获得耐热突变型DNA聚合酶的方法，其中所述耐热突变体多肽是从栖热水栖菌获得的DNA聚合酶I的变体; 多核苷酸，表达载体和编码耐热突变型DNA聚合酶的宿主细胞; 以及利用耐热突变型DNA聚合酶进行逆转录聚合酶链反应（RT-PCR）的方法，以及用于促进其的试剂盒。
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公开(公告)号：US20050250131A1
公开(公告)日：2005-11-10
申请号：US11065943
申请日：2005-02-25
CPC分类号： C07K14/43563 , A61K38/00 , C12N9/1252 , Y02A50/473
摘要： The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.
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6.发明申请Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same 有权用于获得热稳定酶的方法，具有新催化活性的水生栖热菌的DNA聚合酶I变体，获得其的方法及其应用公开(公告)号：US20050191635A1
公开(公告)日：2005-09-01
申请号：US10787219
申请日：2004-02-27
CPC分类号： C07K14/43563 , A61K38/00 , C12N9/1252 , Y02A50/473
摘要： The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.
摘要翻译： 本发明提供一种获得耐热性酶的方法。 本发明还提供了水生栖热菌的DNA聚合酶I的变体。 本发明还提供鉴定具有增强的催化活性的突变型DNA聚合酶的方法。 本发明还提供了编码突变型DNA聚合酶的多核苷酸，表达系统和宿主细胞。 此外，本发明提供了进行逆转录聚合酶链反应（RT-PCR）和试剂盒以促进其的方法。
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公开(公告)号：US08680018B2
公开(公告)日：2014-03-25
申请号：US12246741
申请日：2008-10-07
IPC分类号： C40B30/06
CPC分类号： C40B40/02 , C07K2319/02 , C07K2319/41 , C07K2319/43 , C07K2319/50 , C07K2319/70 , C07K2319/735 , C12N15/1037 , C12N15/62
摘要： The present invention concerns a method for the selection of a virus comprising the steps of: (a) providing a virus encoding and displaying a fusion polypeptide, said fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a viral coat protein polypeptide, wherein said virus comprises a cleavable site located within a displayed polypeptide; (b) exposing the virus to a cleaving agent; (c) propagating the virus comprising intact fusion protein.
摘要翻译： 本发明涉及一种选择病毒的方法，包括以下步骤：（a）提供编码和显示融合多肽的病毒，所述融合多肽包含插入到病毒外壳蛋白多肽序列中的异源多肽，其中所述 病毒包含位于显示的多肽内的可切割位点; （b）将病毒暴露于裂解剂; （c）扩增包含完整融合蛋白的病毒。
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8.发明授权Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same 有权用于获得热稳定酶的方法，具有新催化活性的水生栖热菌的DNA聚合酶I变体，获得其的方法及其应用公开(公告)号：US08927699B2
公开(公告)日：2015-01-06
申请号：US10590810
申请日：2005-02-25
IPC分类号： C12N9/12 , C07H21/02 , C07K14/435 , A61K38/00
CPC分类号： C07K14/43563 , A61K38/00 , C12N9/1252 , Y02A50/473
摘要： A thermostable mutant DNA polymerase; a method for obtaining the thernmostable mutant DNA polymerase by identifying a thermostable mutant polypeptide exhibiting enzymatic activity, wherein the thermostable mutant polypeptide is a variant of DNA polymerase I obtained from Thermus aquaticus; a polynucleotide, an expression vector, and a host cell encoding the thermostable mutant DNA polymerase; and a method of performing a reverse transcription polymerase chain reaction (RT-PCR) utilizing the thermostable mutant DNA polymerase, as well as a kit for facilitating the same.
摘要翻译： 热稳定突变体DNA聚合酶; 通过鉴定表现出酶活性的热稳定突变体多肽来获得耐热突变型DNA聚合酶的方法，其中所述耐热突变体多肽是从栖热水栖菌获得的DNA聚合酶I的变体; 多核苷酸，表达载体和编码耐热突变型DNA聚合酶的宿主细胞; 以及利用耐热突变型DNA聚合酶进行逆转录聚合酶链反应（RT-PCR）的方法，以及用于促进其的试剂盒。
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公开(公告)号：US20090203542A1
公开(公告)日：2009-08-13
申请号：US12246741
申请日：2008-10-07
CPC分类号： C40B40/02 , C07K2319/02 , C07K2319/41 , C07K2319/43 , C07K2319/50 , C07K2319/70 , C07K2319/735 , C12N15/1037 , C12N15/62
摘要： The present invention concerns a method for the selection of a virus comprising the steps of: (a) providing a virus encoding and displaying a fusion polypeptide, said fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a viral coat protein polypeptide, wherein said virus comprises a cleavable site located within a displayed polypeptide; (b) exposing the virus to a cleaving agent; (c) propagating the virus comprising intact fusion protein.
摘要翻译： 本发明涉及一种选择病毒的方法，包括以下步骤：（a）提供编码和显示融合多肽的病毒，所述融合多肽包含插入到病毒外壳蛋白多肽序列中的异源多肽，其中所述 病毒包含位于显示的多肽内的可切割位点; （b）将病毒暴露于裂解剂; （c）扩增包含完整融合蛋白的病毒。
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