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    Method for detecting adeno-associated virus
    5.
    发明授权
    Method for detecting adeno-associated virus 有权
    腺相关病毒检测方法

    公开(公告)号：US08524446B2

    公开(公告)日：2013-09-03

    申请号：US12962793

    申请日：2010-12-08

    申请人： Guangping Gao ,  James M Wilson ,  Mauricio R. Alvira

    发明人： Guangping Gao ,  James M Wilson ,  Mauricio R. Alvira

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N15/86 ,  A61K38/177 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/14121 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2750/14171 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2830/90 ,  C12Q1/701 ,  C12Q2600/158 ,  C12Y304/21022

    摘要： A method for detecting and isolating AAV sequences in a sample of DNA obtained from tissue or cells is provided, which sample contains DNA and proviral AAV. The method involves subjecting the sample containing DNA to amplification via polymerase chain reaction (PCR) using a first set of primers which specifically amplify a first AAV region. The first AAV region is characterized by having at least 250 nucleotides of AAV capsid nucleic acid sequence, a variable sequence flanked by a sequence of at least 18 nucleotides at the 5′ end of the first AAV region and a sequence of at least 18 nucleotides at the 3′ end of the first AAV region. Each of the 5′ and 3′ at least 18 nucleotides is the same over at least 9 consecutive nucleotides relative to corresponding sequences in an alignment of at least two AAV serotypes. Each of the sets of primers consist of a 5′ primer and a 3′ primer. The method is further useful for identifying AAV sequences in the sample by the presence of amplified proviral AAV sequences.

    摘要翻译： 提供了从组织或细胞获得的DNA样品中检测和分离AAV序列的方法，该样品含有DNA和前病毒AAV。 该方法包括使用特异性扩增第一AAV区域的第一组引物通过聚合酶链反应（PCR）使含有DNA的样品进行扩增。 第一个AAV区的特征在于具有AAV衣壳核酸序列的至少250个核苷酸，在第一AAV区的5'末端侧接至少18个核苷酸的序列的可变序列和至少18个核苷酸的序列 第一个AAV区域的3'端。 相对于至少两种AAV血清型的比对中的相应序列，5'和3'至少18个核苷酸中的每一个在至少9个连续核苷酸上相同。 每组引物由5'引物和3'引物组成。 该方法进一步用于通过扩增的前病毒AAV序列的存在来鉴定样品中的AAV序列。

    ADENO-ASSOCIATED VIRUS (AAV) SEQUENCES AND ISOLATING NOVEL SEQUENCES IDENTIFIED THEREBY
    9.
    发明申请
    ADENO-ASSOCIATED VIRUS (AAV) SEQUENCES AND ISOLATING NOVEL SEQUENCES IDENTIFIED THEREBY 有权
    ADENO相关病毒（AAV）序列和分离的序列识别

    公开(公告)号：US20110151434A1

    公开(公告)日：2011-06-23

    申请号：US12962793

    申请日：2010-12-08

    申请人： GUANGPING GAO ,  James M. Wilson ,  Mauricio R. Alvira

    发明人： GUANGPING GAO ,  James M. Wilson ,  Mauricio R. Alvira

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N15/63

    CPC分类号： C12N15/86 ,  A61K38/177 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/14121 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2750/14171 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2830/90 ,  C12Q1/701 ,  C12Q2600/158 ,  C12Y304/21022

    摘要： A method for detecting and isolating AAV sequences in a sample of DNA obtained from tissue or cells is provided, which sample contains DNA and proviral AAV. The method involves subjecting the sample containing DNA to amplification via polymerase chain reaction (PCR) using a first set of primers which specifically amplify a first AAV region. The first AAV region is characterized by having at least 250 nucleotides of AAV capsid nucleic acid sequence, a variable sequence flanked by a sequence of at least 18 nucleotides at the 5′ end of the first AAV region and a sequence of at least 18 nucleotides at the 3′ end of the first AAV region. Each of the 5′ and 3′ at least 18 nucleotides is the same over at least 9 consecutive nucleotides relative to corresponding sequences in an alignment of at least two AAV serotypes. Each of the sets of primers consist of a 5′ primer and a 3′ primer. The method is further useful for identifying AAV sequences in the sample by the presence of amplified proviral AAV sequences.

    摘要翻译： 提供了从组织或细胞获得的DNA样品中检测和分离AAV序列的方法，该样品含有DNA和前病毒AAV。 该方法包括使用特异性扩增第一AAV区域的第一组引物通过聚合酶链反应（PCR）使含有DNA的样品进行扩增。 第一个AAV区的特征在于具有AAV衣壳核酸序列的至少250个核苷酸，在第一AAV区的5'末端侧接至少18个核苷酸的序列的可变序列和至少18个核苷酸的序列 第一个AAV区域的3'端。 相对于至少两种AAV血清型的比对中的相应序列，5'和3'至少18个核苷酸中的每一个在至少9个连续核苷酸上相同。 每组引物由5'引物和3'引物组成。 该方法进一步用于通过扩增的前病毒AAV序列的存在来鉴定样品中的AAV序列。