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公开(公告)号：US5346999A
公开(公告)日：1994-09-13
申请号：US328471
申请日：1989-03-24
CPC分类号： C12N9/0012 , B01J19/0046 , C12M47/06 , C12M47/12 , C12N15/1003 , B01J2219/00286 , B01J2219/00308 , B01J2219/00331 , B01J2219/00389 , B01J2219/00488 , B01J2219/00689 , B01J2219/00695 , C40B60/14 , Y10T436/25125 , Y10T436/25375
摘要： An automated apparatus is provided which implements a new method of extracting and purifying nucleic acids from cells without the use of centrifugation. In the method, a lysate is created by treating the cells with proteinase K in the presence of a lysis buffer having a high concentration of a salt. The lysate is mixed with a phenol-based solvent system, thereby creating an emulsion. The emulsion is heated to promote phase separation. Similarly, the rate of phase separation is also enhanced by increasing the surface area of the emulsion. Once the phase separation is complete, the lower organic phase is removed and the upper aqueous phase is repeatedly extracted with the phenol-based solvent a preselected number of times, and is finally extracted using chloroform. The remaining aqueous phase is then dialyzed to further purify and concentrate the nucleic acid solution. Two preferred embodiments of apparatus are presented to accomplish this extraction.
摘要翻译： 提供了一种自动化装置，其实现了从细胞中提取和纯化核酸而不使用离心的新方法。 在该方法中，通过在具有高浓度盐的裂解缓冲液存在下用蛋白酶K处理细胞产生裂解物。 将裂解物与苯酚基溶剂体系混合，从而产生乳液。 将乳液加热以促进相分离。 类似地，通过增加乳液的表面积也增加了相分离速率。 一旦相分离完成，除去较低的有机相，并用苯酚类溶剂预选次数反复提取上部水相，最后用氯仿萃取。 然后将剩余的水相透析以进一步净化并浓缩核酸溶液。 呈现装置的两个优选实施例以完成该提取。
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公开(公告)号：US5338426A
公开(公告)日：1994-08-16
申请号：US936979
申请日：1992-08-28
IPC分类号： G01N27/447 , C25B9/00
CPC分类号： G01N27/44704
摘要： An electrophoresis apparatus has a gel film cast between two plates and buffer reservoirs at each end of the film with electrodes connectable to an external power supply for providing electromotive force for driving electrophoresis. The reservoirs are configured to wet the ends of the gel film and submerge the electrodes with the apparatus positioned either horizontally or vertically, so gel films can be cast horizontally with sample wells formed in the end of the gel between the plates. Samples may be added to the wells and run into the gel with the apparatus positioned vertically, and the analytical separation may be performed with the apparatus again positioned horizontally, such as in an automatic scanning apparatus.
摘要翻译： 电泳装置具有在两个板之间的凝胶膜和在膜的每个端部的缓冲储存器，其电极可连接到外部电源，以提供用于驱动电泳的电动势。 储存器被配置为润湿凝胶膜的端部并使设备浸没在水平或垂直方向上，因此凝胶膜可以在形成在凝胶的端部之间的样品阱之间水平地铸造。 可以将样品加入到孔中，并使装置垂直放置在凝胶中，并且可以在水平再次定位的装置上进行分析分离，例如在自动扫描装置中。
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