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    Process and device for the parallel preparation of at least 4n oligonucleotides
    3.
    发明授权
    Process and device for the parallel preparation of at least 4n oligonucleotides 有权
    用于并行制备至少4n寡核苷酸的方法和装置

    公开(公告)号：US06866827B2

    公开(公告)日：2005-03-15

    申请号：US10040082

    申请日：2001-10-26

    申请人： Ludwig Stöckl

    发明人： Ludwig Stöckl

    IPC分类号： B01J19/00 ,  C07B61/00 ,  C07H21/00 ,  C40B40/06 ,  C40B60/14 ,  B01J8/00

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00326 ,  B01J2219/00331 ,  B01J2219/00351 ,  B01J2219/00389 ,  B01J2219/00461 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  Y02P20/55

    摘要： In a process and a device for the parallel preparation of at least 4n oligonucleotides, at least four inserts each with n reaction vessels are first arranged on a plate (16), each reaction vessel containing a nucleotide initiator base bound to an inert carrier. Particular operations are then carried out in parallel with one another at four stations (28, 30, 32, 34), and in particular a deblocking operation simultaneously in all n reaction vessels of the insert at the first station (28), a first washing operation simultaneously in all n reaction vessels of the insert at the second station (30), a coupling operation in all n reaction vessels of the insert at the third station (32), and, simultaneously in all n reaction vessels of the insert at the fourth station (34), a second washing operation followed by a capping operation followed by a third washing operation followed by an oxidation operation followed by a fourth washing operation. The plate (16) with the inserts is rotated station by station, the above-mentioned operations being carried out, until the desired oligonucleotides have been formed by coupling individual nucleotides to one another.

    摘要翻译： 在用于并行制备至少4n寡核苷酸的方法和装置中，首先将至少四个具有n个反应容器的插入物排列在板（16）上，每个反应容器含有与惰性载体结合的核苷酸引发剂碱基。 特别是在四个站（28,30,32,34）处并行地进行特别的操作，特别是在第一站（28）的插入件的所有n个反应容器中同时进行去块操作，第一洗涤 在第二站（30）的插入物的所有n个反应容器中同时操作，在第三站（32）处插入物的所有n个反应容器中的耦合操作，以及在插入物的所有n个反应容器 第四站（34），第二清洗操作，接着进行封盖操作，随后进行第三洗涤操作，随后进行氧化操作，随后进行第四次清洗操作。 具有插入物的板（16）逐站地旋转，进行上述操作，直到通过将各个核苷酸彼此连接而形成所需的寡核苷酸。

    Dual Mobile Phase Apparatus and Method
    6.
    发明申请
    Dual Mobile Phase Apparatus and Method 审中-公开
    双流动相设备及方法

    公开(公告)号：US20160158725A1

    公开(公告)日：2016-06-09

    申请号：US15040854

    申请日：2016-02-10

    申请人： Swedish Biomimetics 3000 Limited

    发明人： Andrew Richard Russell PREWER

    IPC分类号： B01J19/24 ,  B01J19/00

    CPC分类号： B01J19/24 ,  B01J19/0006 ,  B01J19/0046 ,  B01J2219/00058 ,  B01J2219/00164 ,  B01J2219/00274 ,  B01J2219/00331 ,  B01J2219/00454 ,  B01J2219/00497 ,  B01J2219/00515 ,  B01J2219/00518 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00655 ,  B01J2219/00675 ,  B01J2219/00686 ,  B01J2219/00725 ,  B01J2219/00871 ,  B01J2219/24 ,  B82Y30/00 ,  C07K1/042 ,  C07K1/045 ,  C07K1/047 ,  C40B60/14

    摘要： An apparatus and system for contacting a mobile elongate solid phase, e.g. a ribbon with a flowing fluid phase, and a method for using the same in, for example solid phase synthesis. A particular apparatus comprises (i) a conduit which is of circular or non-circular transverse cross section and which defines a lumen to contain both the flowing fluid phase and the mobile elongate solid phase; (ii) fluid phase ports in communication with the lumen to allow the fluid phase to enter the lumen, flow through it and exit it; and (iii) solid phase ports in communication with the lumen to allow the mobile solid phase to enter the lumen, move through it and exit it, the apparatus being adapted to prevent fluid egress from its interior through the solid phase ports.Also provided is a continuous process for synthesising and screening molecules, comprising passing an elongate solid phase through successive treatment stations where different stages of a solid phase synthesis are performed, and screening the synthesised molecules for activity.

    摘要翻译： 一种用于接触移动细长固相的装置和系统，例如， 具有流动相的流动相的带，以及在固相合成中使用该带的方法。 特定装置包括（i）具有圆形或非圆形横截面并且限定内腔以容纳流动流体相和移动细长固体相的管道; （ii）与内腔连通以允许流体相进入管腔的流体相口，流过其并将其排出; 和（iii）与内腔连通的固相端口，以允许移动固相进入管腔，移动通过其并将其排出，该装置适于防止流体从其内部通过固相端口流出。 还提供了用于合成和筛选分子的连续方法，包括使细长的固相通过连续的处理站，其中进行固相合成的不同阶段，并筛选合成的分子的活性。

    HIGH-DENSITY MICROCHAMBER ARRAY AND MANUFACTURING METHOD THEREOF
    7.
    发明申请
    HIGH-DENSITY MICROCHAMBER ARRAY AND MANUFACTURING METHOD THEREOF 审中-公开
    高密度MICROCHAMBER阵列及其制造方法

    公开(公告)号：US20160296928A1

    公开(公告)日：2016-10-13

    申请号：US14913470

    申请日：2014-08-18

    申请人： THE UNIVERSITY OF TOKYO

    发明人： Hiroyuki NOJI ,  Rikiya WATANABE ,  Hiroaki SUGA ,  Daishi FUJITA

    IPC分类号： B01L3/00 ,  B01J19/00

    CPC分类号： B01L3/5085 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00331 ,  B01J2219/00662 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01L3/502769 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/165 ,  G01N33/48721

    摘要： A material film is formed as a thin film having a thickness of 1 μm on a surface of a glass substrate. A plurality of micro-chambers having a diameter of 5 μm are formed in the material film to be arrayed at a high density. The respective chambers filled with an aqueous test solution have openings that are liquid-sealed by a lipid bilayer membrane to provide a high-density micro-chamber array. Significant downsizing of the micro-chambers enhances a change in concentration by a reaction of one biomolecule in the chamber and thereby increases the detection sensitivity. In the configuration that a large number of micro-chambers are formed at a high density, even in the case of an extremely slow reaction of the biomolecule, the reaction proceeds in any of the chambers. This configuration accordingly enables the reaction of the biomolecule to be detected with high sensitivity.

    摘要翻译： 在玻璃基板的表面上形成厚度为1μm的薄膜。 在材料膜中形成多个直径为5μm的微室，以高密度排列。 填充有水性测试溶液的各个室具有通过脂质双层膜液体密封以提供高密度微室阵列的开口。 微室的显着缩小通过室中的一个生物分子的反应增强了浓度的变化，从而增加了检测灵敏度。 在以高密度形成大量微室的结构中，即使在生物分子反应极慢的情况下，也可以在任何室内进行反应。 因此，能够高灵敏度地检测生物分子的反应。

    DUAL MOBILE PHASE APPARATUS AND METHOD
    8.
    发明申请
    DUAL MOBILE PHASE APPARATUS AND METHOD 有权
    双移动相位装置和方法

    公开(公告)号：US20100120636A1

    公开(公告)日：2010-05-13

    申请号：US12683272

    申请日：2010-01-06

    申请人： Andrew Richard Russell Prewer

    发明人： Andrew Richard Russell Prewer

    IPC分类号： C40B50/14 ,  C40B60/08

    CPC分类号： B01J19/24 ,  B01J19/0006 ,  B01J19/0046 ,  B01J2219/00058 ,  B01J2219/00164 ,  B01J2219/00274 ,  B01J2219/00331 ,  B01J2219/00454 ,  B01J2219/00497 ,  B01J2219/00515 ,  B01J2219/00518 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00655 ,  B01J2219/00675 ,  B01J2219/00686 ,  B01J2219/00725 ,  B01J2219/00871 ,  B01J2219/24 ,  B82Y30/00 ,  C07K1/042 ,  C07K1/045 ,  C07K1/047 ,  C40B60/14

    摘要： An apparatus and system for contacting a mobile elongate solid phase, e.g. a ribbon with a flowing fluid phase, and a method for using the same in, for example solid phase synthesis. A particular apparatus comprises (i) a conduit which is of circular or non-circular transverse cross section and which defines a lumen to contain both the flowing fluid phase and the mobile elongate solid phase; (ii) fluid phase ports in communication with the lumen to allow the fluid phase to enter the lumen, flow through it and exit it; and (iii) solid phase ports in communication with the lumen to allow the mobile solid phase to enter the lumen, move through it and exit it, the apparatus being adapted to prevent fluid egress from its interior through the solid phase ports.Also provided is a continuous process for synthesising and screening molecules, comprising passing an elongate solid phase through successive treatment stations where different stages of a solid phase synthesis are performed, and screening the synthesised molecules for activity.

    摘要翻译： 一种用于接触移动细长固相的装置和系统，例如， 具有流动相的流动相的带，以及在固相合成中使用该带的方法。 特定装置包括（i）具有圆形或非圆形横截面并且限定内腔以容纳流动流体相和移动细长固体相的管道; （ii）与内腔连通以允许流体相进入管腔的流体相口，流过其并将其排出; 和（iii）与内腔连通的固相端口，以允许移动固相进入管腔，移动通过其并将其排出，该装置适于防止流体从其内部通过固相端口流出。 还提供了用于合成和筛选分子的连续方法，包括使细长的固相通过连续的处理站，其中进行固相合成的不同阶段，并筛选合成的分子的活性。

    Reactor assembly
    9.
    发明申请
    Reactor assembly 有权
    反应器总成

    公开(公告)号：US20040202573A1

    公开(公告)日：2004-10-14

    申请号：US10477028

    申请日：2004-04-23

    发明人： Peter John van den Brink ,  Maarten Bracht ,  Gerardus Johannes Maria Gruter ,  Rene du Ruiter ,  Bashir Husein Harji

    IPC分类号： G01N033/00

    CPC分类号： B01L3/5025 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/00308 ,  B01J2219/00313 ,  B01J2219/00331 ,  B01J2219/00333 ,  B01J2219/00351 ,  B01J2219/00423 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00745 ,  B01J2219/00747 ,  B01L2300/04 ,  B01L2300/0867 ,  C40B30/08 ,  C40B40/18 ,  C40B60/12 ,  C40B60/14 ,  G01N1/405 ,  G01N31/10

    摘要： Described is a Reactor assembly for analysing the effluent stream from at least one flownullthrough reactor, comprising: nullat least one flow-through reactor for performing at least one chemical reaction, the reactor comprising nulla reaction chamber (1), comprising a reaction zone (2), the reaction chamber being connected to nullat least one reactor inlet (3) for at least one reactant, 10 upstream of the reaction zone, nullat least one reactor outlet (4) for the effluent stream from the reaction zone, downstream of the reaction zone, nullat least one analyser (5) for subjecting the effluent stream to an analysing procedure, each reactor outlet being 15 connected to at least one analyser by an effluent conduit (6), wherein the reactor assembly further comprises nullat least one dilution fluid supply means (10), for adding at least one dilution fluid to the effluent stream, downstream of the reaction zone, and a process for analysing a fluid effluent stream from a flow-through reactor comprising a reaction chamber having a reaction zone, comprising the steps of: A) diluting at least 30 w/w % and/or at least 30 v/v % effluent stream downstream of the reaction zone with at least one 25 dilution fluid, B) transferring at least a portion of the diluted effluent stream obtained in step A) to at least one analyser, C) subjecting the transferred effluent stream to an analysis procedure.

    摘要翻译： 描述了一种用于分析来自至少一个流通反应器的流出物流的反应器组件，包括：至少一个用于进行至少一个化学反应的流通反应器，所述反应器包括反应室（1），其包括 反应区（2），反应室连接至至少一个反应器入口（3），用于至少一个反应物，10个反应区上游， - 至少一个反应器出口（4）用于来自反应的流出物流 在反应区下游的至少一个分析器（5），用于使流出物流进行分析程序，每个反应器出口通过流出物导管（6）连接至至少一个分析器，其中反应器组件进一步 包括 - 至少一个稀释液供应装置（10），用于在反应区的下游向流出物流中加入至少一种稀释流体，以及用于分析来自包含反应物的流通反应器的流体流出物流的方法 室具有反应区，包括以下步骤：A）用至少一种25稀释流体稀释反应区下游的至少30w / w％和/或至少30v / v％流出物流，B）在 将至少一部分在步骤A）中获得的稀释流出物流送至至少一个分析器，C）使转移的流出物流进行分析程序。