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    Method of nucleic acid extraction
    5.
    发明授权
    Method of nucleic acid extraction 失效
    核酸提取方法

    公开(公告)号：US5346999A

    公开(公告)日：1994-09-13

    申请号：US328471

    申请日：1989-03-24

    申请人： Guy R. Cathcart ,  Paul D. Grossman ,  P. Eric Mayrand ,  Eric S. Nordman ,  Norman M. Whiteley

    发明人： Guy R. Cathcart ,  Paul D. Grossman ,  P. Eric Mayrand ,  Eric S. Nordman ,  Norman M. Whiteley

    IPC分类号： B01J19/00 ,  C12M1/00 ,  C12N9/06 ,  C12N15/10 ,  C40B60/14 ,  C07H23/00

    CPC分类号： C12N9/0012 ,  B01J19/0046 ,  C12M47/06 ,  C12M47/12 ,  C12N15/1003 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00331 ,  B01J2219/00389 ,  B01J2219/00488 ,  B01J2219/00689 ,  B01J2219/00695 ,  C40B60/14 ,  Y10T436/25125 ,  Y10T436/25375

    摘要： An automated apparatus is provided which implements a new method of extracting and purifying nucleic acids from cells without the use of centrifugation. In the method, a lysate is created by treating the cells with proteinase K in the presence of a lysis buffer having a high concentration of a salt. The lysate is mixed with a phenol-based solvent system, thereby creating an emulsion. The emulsion is heated to promote phase separation. Similarly, the rate of phase separation is also enhanced by increasing the surface area of the emulsion. Once the phase separation is complete, the lower organic phase is removed and the upper aqueous phase is repeatedly extracted with the phenol-based solvent a preselected number of times, and is finally extracted using chloroform. The remaining aqueous phase is then dialyzed to further purify and concentrate the nucleic acid solution. Two preferred embodiments of apparatus are presented to accomplish this extraction.

    摘要翻译： 提供了一种自动化装置，其实现了从细胞中提取和纯化核酸而不使用离心的新方法。 在该方法中，通过在具有高浓度盐的裂解缓冲液存在下用蛋白酶K处理细胞产生裂解物。 将裂解物与苯酚基溶剂体系混合，从而产生乳液。 将乳液加热以促进相分离。 类似地，通过增加乳液的表面积也增加了相分离速率。 一旦相分离完成，除去较低的有机相，并用苯酚类溶剂预选次数反复提取上部水相，最后用氯仿萃取。 然后将剩余的水相透析以进一步净化并浓缩核酸溶液。 呈现装置的两个优选实施例以完成该提取。

    Methods and Apparatus for Delivering Feedstocks for Plasma Treatment
    7.
    发明公开
    Methods and Apparatus for Delivering Feedstocks for Plasma Treatment 审中-公开

    公开(公告)号：US20240024840A1

    公开(公告)日：2024-01-25

    申请号：US18027082

    申请日：2021-09-08

    申请人： Haydale Graphene Industries PLC

    发明人： John-Mark Seymour ,  Thomas Howe ,  Lee Edwards

    IPC分类号： B01J19/08

    CPC分类号： B01J19/088 ,  B01J2219/0898 ,  B01J2219/00488 ,  B01J2219/00355 ,  B01J2219/00186 ,  B01J2219/00162

    摘要： The present application relates to methods and apparatus for delivering liquid or solid feedstocks into a plasma treatment vessel. More specifically, the invention provides a method for treating a sample using glow discharge plasma in an apparatus comprising a treatment vessel, the method comprising (i) delivering a gaseous plasma forming feedstock into the treatment vessel through a gas supply line under the control of a gas flow controller, and causing formation of a glow discharge plasma in the treatment vessel from the gaseous plasma forming feedstock; and simultaneously (ii) delivering a reagent into the treatment vessel under the control of a reagent dosing controller, wherein the reagent is a liquid or a solid; and (iii) contacting the sample with the glow discharge plasma and the reagent; wherein the gas flow controller and the reagent dosing controller allow independent control of the rate of delivery of the gaseous plasma forming feedstock and the reagent.