公开(公告)号：US20230407262A1

公开(公告)日：2023-12-21

申请号：US18055409

申请日：2022-11-14

Applicant: Gwo Xi Stem Cell Applied Technology Co., Ltd.

Inventor： Wei Wu Li ,  Tzyy-Wen Chiou ,  Horng-Jyh Harn ,  Shinn-Zong Lin ,  Ming-Hsi Chuang ,  Po-Cheng Lin ,  Chia-Hsin Lee ,  Mao-Hsuan Huang

IPC: C12N5/0775 ,  C12N5/079 ,  C07K14/435 ,  A61K38/17 ,  A61P25/00

CPC classification number: C12N5/0663 ,  C12N5/0618 ,  C07K14/435 ,  A61K38/17 ,  A61P25/00

Abstract: The present invention provides a medicine for treating a neurodegenerative disease, which employs LEFTY2 (Left-Right Determination Factor 2) generated by co-culturing mesenchymal stem cells of mammals with nerve cells having mutations in the APP (Amyloid precursor protein) gene, or a specific protein. The LEFTY2 has an effect of inhibiting Beta amyloid and a hyperphosphorylated neuronal microtubule-associated protein (Tau protein), without affecting the development of nerve cells and having the ability to promote the growth of the nerve cells; and the two types of proteins have a crucial impact on the neurodegenerative diseases.

公开(公告)号：US20230193198A1

公开(公告)日：2023-06-22

申请号：US18066999

申请日：2022-12-15

Applicant: Gwo Xi Stem Cell Applied Technology Co., Ltd.

Inventor： Ming-Hsi Chuang ,  Po-Cheng Lin ,  Chia-Hsin Lee ,  Mao-Hsuan Huang

IPC: C12N5/074

CPC classification number: C12N5/0607 ,  C12N2533/74 ,  C12N2500/98 ,  C12N2500/12 ,  C12N2500/32 ,  C12N2523/00

Abstract: A method for stably amplifying a pluripotent stem cell comprises the following steps: (a) a cell implantation step: implanting pluripotent stem cells directly into a porous scaffold such that the porous scaffold contains 1×104 or more of the pluripotent stem cells; and (b) a cell amplification step: immersing the porous scaffold in a specific culture medium which is xeno-free (XF) and performing amplification culture at an ambient temperature of 35.5-39.5° C. and a CO2 concentration of 5% to obtain the amplified pluripotent stem cells, wherein the amplified pluripotent stem cells aggregate to present an embryoid body state. The amplification method of the present disclosure can easily obtain an excellent effect of increasing an amplification multiple of the pluripotent stem cells to about 3 times or more.