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公开(公告)号：US06592821B1
公开(公告)日：2003-07-15
申请号：US09853887
申请日：2001-05-10
申请人： H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow , Yevgeny Yurkovetsky , Javier Anibal Farinas
发明人： H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow , Yevgeny Yurkovetsky , Javier Anibal Farinas
IPC分类号： G01N3348
CPC分类号： B01F13/0062 , B01J19/0093 , B01L3/502776 , B01L2200/0636 , B01L2400/0406 , B01L2400/0487 , G01N15/1404 , G01N27/44704 , G01N27/44791 , G01N2015/1409 , G01N2015/149 , G01N2333/4718 , Y10T436/11
摘要： Methods and systems for particle focusing to increase assay throughput and sensitivity in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.
摘要翻译： 提供了粒子聚焦的方法和系统，以增加微量体系中的分析产量和灵敏度。 本发明包括为微流体装置中的流动颗粒提供基本均匀的流速的方法。 还提供了分选颗粒群体成员的方法，例如细胞和各种亚细胞组分。 另外包括其中聚焦和/或分选颗粒的集成系统。
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2.发明授权Use of Nernstein voltage sensitive dyes in measuring transmembrane voltage 有权使用Nernstein电压敏感染料测量跨膜电压公开(公告)号：US06979553B2
公开(公告)日：2005-12-27
申请号：US10655697
申请日：2003-09-05
IPC分类号： G01N21/64 , C12M1/34 , C12Q1/02 , G01N1/30 , G01N21/78 , G01N31/22 , G01N33/50 , G01N37/00 , C12Q1/00 , G01N33/53
CPC分类号： G01N31/22 , G01N1/30 , G01N33/5005 , G01N2001/302 , G01N2458/00 , Y10S435/968
摘要： Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
摘要翻译： 提供了使用阳离子染料和阴离子染料的跨膜电位测量方法。 包括染料和膜的阳离子和阴离子染料和微流体系统的组合与跨膜电位测量的处理元件结合提供。
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3.发明授权公开(公告)号：US06537771B1
公开(公告)日：2003-03-25
申请号：US09684313
申请日：2000-10-06
IPC分类号： C12Q102
CPC分类号： G01N31/22 , G01N1/30 , G01N33/5005 , G01N2001/302 , G01N2458/00 , Y10S435/968
摘要： Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
摘要翻译： 提供了使用阳离子染料和阴离子染料的跨膜电位测量方法。 包括染料和膜的阳离子和阴离子染料和微流体系统的组合与跨膜电位测量的处理元件结合提供。
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4.发明授权公开(公告)号：US06759191B2
公开(公告)日：2004-07-06
申请号：US10349396
申请日：2003-01-21
IPC分类号： C12Q100
CPC分类号： G01N31/22 , G01N1/30 , G01N33/5005 , G01N2001/302 , G01N2458/00 , Y10S435/968
摘要： Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
摘要翻译： 提供了使用阳离子染料和阴离子染料的跨膜电位测量方法。 包括染料和膜的阳离子和阴离子染料和微流体系统的组合与跨膜电位测量的处理元件结合提供。
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