公开(公告)号：US20180272349A1

公开(公告)日：2018-09-27

申请号：US15762274

申请日：2016-03-28

Applicant: Hitachi, Ltd.

Inventor： Tomoyuki SAKAI ,  Masataka SHIRAI

IPC: B01L3/00 ,  C12N15/10

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0893 ,  B01L2400/086 ,  C12M1/34 ,  C12N15/1013

Abstract: A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.

公开(公告)号：US20240416344A1

公开(公告)日：2024-12-19

申请号：US18583000

申请日：2024-02-21

Applicant: Hitachi, Ltd.

Inventor： Saaya SHIMIZU ,  Tomoyuki SAKAI ,  Yoshimitsu YANAGAWA

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6876

Abstract: Separation of metal nanoparticles and detection of a gene in a fluid from which the metal nanoparticles are separated are performed in different spaces. A fluid device includes: a gene amplification section that stores a fluid containing metal nanoparticles and that heats the fluid using a photothermal effect of the metal nanoparticles to amplify a gene in the fluid; a metal nanoparticles separating section that separates the metal nanoparticles from the fluid; and a gene detection section that is a space different from the metal nanoparticles separating section and that detects the gene in the fluid from which the metal nanoparticles are separated in the metal nanoparticles separating section.

公开(公告)号：US20180304262A1

公开(公告)日：2018-10-25

申请号：US15544888

申请日：2015-02-03

Applicant: HITACHI, LTD.

Inventor： Masataka SHIRAI ,  Tomoyuki SAKAI

IPC: B01L3/00 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/22 ,  C12N15/10

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  C12M1/00 ,  C12N15/1003 ,  C12N15/1096 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/22

Abstract: (1) Plural reaction chambers each including at least one cell capturing unit and at least one nucleic acid capturing unit connected via a flow channel, (2) a first liquid flow channel commonly connected to the plural reaction chambers on a first surface side, (3) plural second liquid flow channels connected to the plural reaction chambers in a one-on-one relationship on the first surface side, and (4) a third liquid flow channel commonly connected to the plural reaction chambers on a second surface side opposite to the first surface, are provided in a flow cell device for single cell analysis.

公开(公告)号：US20180119135A1

公开(公告)日：2018-05-03

申请号：US15553175

申请日：2015-04-09

Applicant: Hitachi, Ltd.

Inventor： Maiko TANABE ,  Masataka SHIRAI ,  Tomoyuki SAKAI

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12M1/00 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2563/179

Abstract: In order to interpret an arbitrary sequence region in many genes in many cells, it is necessary to degrade a nucleic acid into fragments and introduce a sequence that is different from one cell to another into each of the fragments. However, in the conventional configuration for analyzing many cells, there has been a problem that mixing of the degraded fragments among areas occurs before a tag sequence unique for each of the areas is introduced. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of plural areas on a substrate and synthesizing a complementary DNA (cDNA) of the nucleic acid for each of the areas, wherein the system also includes a means for immediately introducing a tag sequence unique for each of the areas to the reaction product.

公开(公告)号：US20190194645A1

公开(公告)日：2019-06-27

申请号：US16329682

申请日：2017-03-06

Applicant: Hitachi, Ltd.

Inventor： Tomoyuki SAKAI ,  Masataka SHIRAI

IPC: C12N15/10

CPC classification number: C12N15/1013 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09

Abstract: A device is provided for capturing a nucleic acid in a single cell, having: a two-dimensional array comprising a substrate, a plurality of single cell capturing holes provided on one surface of the substrate, and a nucleic acid capturing region comprising, on the inside of the substrate, a nucleic acid capturing body, which is configured to capture the nucleic acids extracted from the individual cells respectively captured by the single cell capturing hole; a flow channel which is provided adjacent to the nucleic acid capturing region of the substrate, and is configured to discharge a solution in the nucleic acid capturing region; and a cylindrical structure body which is arranged on the substrate at the time of introducing a cell suspension and encloses a plurality of the single cell capturing holes, wherein the cylindrical structure body is removed after the cells are captured by the single cell capturing holes.