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公开(公告)号：US09567563B2
公开(公告)日：2017-02-14
申请号：US15014585
申请日：2016-02-03
CPC分类号： C12N1/14 , C07K14/38 , C12N9/24 , C12N9/2437 , C12N9/485 , C12N9/62 , C12Y302/01091
摘要： Provided are an Aspergillus mutant strain which can dramatically enhance production of a heterologous enzyme when a saccharifying enzyme gene is transferred into the strain to perform transformation and a transformant having a saccharifying enzyme gene transferred into the Aspergillus mutant strain. The Aspergillus mutant strain has been completely or partially deficient in three genes of Aspergillus oryzae strain HO2 (accession number: NITE BP-01750): a prtR gene coding for a transcription factor; a pepA gene coding for an extracellular acid protease; and a cpI gene coding for an extracellular acid carboxypeptidase.
摘要翻译： 提供了当将糖化酶基因转移到菌株进行转化时，可以显着提高异源酶的产生的曲霉菌突变菌株和将糖化酶基因转移到曲霉菌突变株中的转化体。 曲霉菌突变菌株在米曲霉菌株HO2（登录号：NITE BP-01750）的三个基因中完全或部分缺陷：编码转录因子的prtR基因; 编码细胞外酸性蛋白酶的pepA基因; 和编码细胞外酸羧肽酶的cpI基因。
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公开(公告)号：US09212368B2
公开(公告)日：2015-12-15
申请号：US14600103
申请日：2015-01-20
CPC分类号： C12N15/80 , C07K14/38 , C12N9/2437 , C12N9/2445 , C12N9/2482
摘要： An Aspergillus mutant strain obtained by deleting a ligD gene from an auxotrophic mutant strain of Aspergillus oryzae strain AOK27L.
摘要翻译： 通过从米曲霉菌株AOK27L的营养缺陷型突变菌株中缺失ligD基因获得的曲霉菌突变菌株。
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公开(公告)号：US10563209B2
公开(公告)日：2020-02-18
申请号：US15745061
申请日：2016-07-15
发明人： Satoru Shinkawa , Shigenobu Mitsuzawa , Maiko Tanaka , Masayuki Machida , Hiroko Hagiwara , Hideaki Koike , Mahoko Kuninaga
IPC分类号： C12N15/80 , C12N15/113 , C12N15/63 , C12N1/14 , C12N15/62
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：US10266833B2
公开(公告)日：2019-04-23
申请号：US15745070
申请日：2016-07-15
发明人： Satoru Shinkawa , Shigenobu Mitsuzawa , Maiko Tanaka , Masayuki Machida , Hiroko Hagiwara , Hideaki Koike , Mahoko Kuninaga
摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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公开(公告)号：US09228181B2
公开(公告)日：2016-01-05
申请号：US14323893
申请日：2014-07-03
发明人： Maiko Tanaka , Shigenobu Mitsuzawa , Satoru Shinkawa , Daisuke Shibata , Takeshi Ara , Migiwa Takeda
CPC分类号： C12N9/2445 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided.
摘要翻译： 本发明涉及具有β-葡糖苷酶活性的多肽，其包含SEQ ID NO：1所示的氨基酸序列，包括其中一个或几个氨基酸被缺失，取代或 加入由SEQ ID NO：1表示的氨基酸序列，或包含与SEQ ID NO：1表示的氨基酸序列具有90％以上序列同一性的氨基酸序列的多肽。根据本发明， 衍生自解淀粉解淀粉的葡糖苷酶，编码葡萄糖苷酶的多核苷酸，用于表达葡糖苷酶的表达载体，与该表达载体结合的转化体，以及使用＆bgr生产纤维素降解产物的方法 ; - 葡糖苷酶。
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公开(公告)号：US09688974B2
公开(公告)日：2017-06-27
申请号：US14600098
申请日：2015-01-20
CPC分类号： C12N9/2437 , C12N9/2402 , C12N9/2405 , C12N9/2434 , C12N9/2445 , C12N9/248 , C12N9/2482 , C12N15/80 , C12P21/02 , C12R1/69
摘要： An Aspergillus mutant strain characterized in that it is an auxotrophic mutant strain of Aspergillus oryzae strain AOK27L.
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公开(公告)号：US09353364B2
公开(公告)日：2016-05-31
申请号：US14324281
申请日：2014-07-07
发明人： Maiko Tanaka , Shigenobu Mitsuzawa , Satoru Shinkawa , Daisuke Shibata , Takeshi Ara , Migiwa Takeda
CPC分类号： C12N9/2445 , C12P19/02 , C12P19/14 , C12Y302/01021 , D21C5/005 , Y02P20/52
摘要： The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided.
摘要翻译： 本发明涉及具有β-葡糖苷酶活性的多肽，其包含SEQ ID NO：1所示的氨基酸序列，包括其中一个或几个氨基酸被缺失，取代或 加入由SEQ ID NO：1表示的氨基酸序列，或包含与SEQ ID NO：1所示的氨基酸序列具有91％以上的氨基酸序列的氨基酸序列的多肽。根据本发明， 衍生自解淀粉解淀粉的葡糖苷酶，编码葡萄糖苷酶的多核苷酸，用于表达葡糖苷酶的表达载体，与该表达载体结合的转化体，以及使用＆bgr生产纤维素降解产物的方法 ; - 葡糖苷酶。
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公开(公告)号：US20150203832A1
公开(公告)日：2015-07-23
申请号：US14600098
申请日：2015-01-20
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/2402 , C12N9/2405 , C12N9/2434 , C12N9/2445 , C12N9/248 , C12N9/2482 , C12N15/80 , C12P21/02 , C12R1/69
摘要： An Aspergillus mutant strain characterized in that it is an auxotrophic mutant strain of Aspergillus oryzae strain AOK27L.
摘要翻译： 曲霉属突变菌株，其特征在于其是米曲霉菌株AOK27L的营养缺陷型突变菌株。
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公开(公告)号：US09605249B2
公开(公告)日：2017-03-28
申请号：US14957915
申请日：2015-12-03
CPC分类号： C12N9/2437 , C12Y302/01091
摘要： An object of the invention is to provide cellobiohydrolase with high activity. The activity of cellobiohydrolase can be improved by substituting, with alanine, asparagine that is the amino acid at position 62 from the N-terminal of cellobiohydrolase shown in SEQ ID NO: 1, or asparagine at a position corresponding to the position 62 in an amino acid sequence corresponding to the sequence of the cellobiohydrolase shown in SEQ ID NO: 1.
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公开(公告)号：US20160230140A1
公开(公告)日：2016-08-11
申请号：US15014585
申请日：2016-02-03
CPC分类号： C12N1/14 , C07K14/38 , C12N9/24 , C12N9/2437 , C12N9/485 , C12N9/62 , C12Y302/01091
摘要： Provided are an Aspergillus mutant strain which can dramatically enhance production of a heterologous enzyme when a saccharifying enzyme gene is transferred into the strain to perform transformation and a transformant having a saccharifying enzyme gene transferred into the Aspergillus mutant strain. The Aspergillus mutant strain has been completely or partially deficient in three genes of Aspergillus oryzae strain HO2 (accession number: NITE BP-01750): a prtR gene coding for a transcription factor; a pepA gene coding for an extracellular acid protease; and a cpI gene coding for an extracellular acid carboxypeptidase.
摘要翻译： 提供了当将糖化酶基因转移到菌株进行转化时，可以显着提高异源酶的产生的曲霉菌突变菌株和将糖化酶基因转移到曲霉菌突变株中的转化体。 曲霉菌突变菌株在米曲霉菌株HO2（登录号：NITE BP-01750）的三个基因中完全或部分缺陷：编码转录因子的prtR基因; 编码细胞外酸性蛋白酶的pepA基因; 和编码细胞外酸羧肽酶的cpI基因。
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