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公开(公告)号：US11746324B2
公开(公告)日：2023-09-05
申请号：US17547863
申请日：2021-12-10
Inventor： Benjamin Eithan Reubinoff , Orna Singer
CPC classification number: C12N5/0621 , A61K9/0051 , A61K35/30 , A61P27/02 , C12N2500/38 , C12N2501/115 , C12N2501/15 , C12N2502/13 , C12N2506/02 , C12N2533/52 , C12N2533/54
Abstract: A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGFβ superfamily.
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公开(公告)号：US09658216B2
公开(公告)日：2017-05-23
申请号：US14375195
申请日：2013-01-29
Inventor： Benjamin Eithan Reubinoff , Nurit Yachimovich-Cohen , Limor Matzrafi
CPC classification number: G01N33/56966 , C12N5/0621 , C12N2501/15 , C12N2501/16 , C12N2506/45 , C12N2509/00 , G01N21/6486 , G01N33/52 , G01N33/582 , G01N2021/4704 , G01N2333/46
Abstract: A method of selecting retinal pigmented epithelial (RPE) cells from a mixed population of cells is disclosed. The method comprises: (a) analyzing the cells of the mixed population of cells for at least one of the following parameters: (i) cells which autofluorescence above a predetermined threshold; (ii) cells which express CD81 above a predetermined threshold; and (iii) cells which scatter light perpendicular to a laser beam above a predetermined threshold; and (b) selecting cells which are positive for at least one of the parameters, thereby sorting RPE cells from a mixed population of cells.
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Patent Agency Ranking
公开(公告)号：US20150010922A1
公开(公告)日：2015-01-08
申请号：US14375195
申请日：2013-01-29
Inventor： Benjamin Eithan Reubinoff , Nurit Yachimovich-Cohen , Limor Matzrafi
IPC: G01N33/52
CPC classification number: G01N33/56966 , C12N5/0621 , C12N2501/15 , C12N2501/16 , C12N2506/45 , C12N2509/00 , G01N21/6486 , G01N33/52 , G01N33/582 , G01N2021/4704 , G01N2333/46
Abstract: A method of selecting retinal pigmented epithelial (RPE) cells from a mixed population of cells is disclosed. The method comprises: (a) analyzing the cells of the mixed population of cells for at least one of the following parameters: (i) cells which autofluorescence above a predetermined threshold; (ii) cells which express CD81 above a predetermined threshold; and (iii) cells which scatter light perpendicular to a laser beam above a predetermined threshold; and (b) selecting cells which are positive for at least one of the parameters, thereby sorting RPE cells from a mixed population of cells.
Abstract translation: 公开了从混合细胞群中选择视网膜色素上皮（RPE）细胞的方法。 该方法包括：（a）分析混合细胞群体的细胞中至少一个以下参数：（i）自发荧光高于预定阈值的细胞; （ii）表达CD81高于预定阈值的细胞; 和（iii）将垂直于激光束的光散射到预定阈值以上的单元; 和（b）选择对至少一个参数是阳性的细胞，从而从混合的细胞群中分选RPE细胞。
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公开(公告)号：US11261433B2
公开(公告)日：2022-03-01
申请号：US16071094
申请日：2017-01-31
Inventor： Ithai Waldhorn , Benjamin Eithan Reubinoff
Abstract: An article of manufacture is disclosed which comprises at least two populations of autosomal-identical induced pluripotent stem cells (iPSCs), wherein the complement of sex chromosomes of the first population of the at least two populations is non-identical to the complement of sex chromosomes of the second population of the at least two populations. Uses thereof and methods of generating same are also disclosed.
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公开(公告)号：US11230696B2
公开(公告)日：2022-01-25
申请号：US15748541
申请日：2016-07-28
Inventor： Benjamin Eithan Reubinoff , Orna Singer
Abstract: A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGFβ superfamily.
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公开(公告)号：US12188047B2
公开(公告)日：2025-01-07
申请号：US15771046
申请日：2016-10-26
Inventor： Benjamin Eithan Reubinoff , Orna Singer , Osnat Bohana-Kashtan , Ofer Wiser
IPC: C12N5/079 , C12N5/00 , C12N5/0735 , G01N33/53
Abstract: A method of generating retinal pigment epithelium cells is disclosed. Cell populations comprising same and uses thereof are also disclosed.
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公开(公告)号：US11035857B2
公开(公告)日：2021-06-15
申请号：US15470926
申请日：2017-03-28
Inventor： Benjamin Eithan Reubinoff , Nurit Yachimovich-Cohen , Limor Matzrafi
Abstract: A method of selecting retinal pigmented epithelial (RPE) cells from a mixed population of cells is disclosed. The method comprises: (a) analyzing the cells of the mixed population of cells for at least one of the following parameters: (i) cells which autofluorescence above a predetermined threshold; (ii) cells which express CD81 above a predetermined threshold; and (iii) cells which scatter light perpendicular to a laser beam above a predetermined threshold; and (b) selecting cells which are positive for at least one of the parameters, thereby sorting RPE cells from a mixed population of cells.
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