公开(公告)号：US07195867B2

公开(公告)日：2007-03-27

申请号：US10035861

申请日：2001-12-26

Applicant: Hai Xing Chen

Inventor： Hai Xing Chen

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12N5/0647 ,  A61K48/00 ,  A61K2035/124 ,  C12N5/0641 ,  C12N2510/02 ,  C12Q1/6837 ,  C12Q2565/102 ,  C12Q2565/519 ,  C12Q2537/125 ,  C12Q2537/149

Abstract: A method for sequentially detecting multiple target nucleic acid fragments in a sample includes steps of adding a sample into a column having a test snare which has thereon multiple single strand capture DNA sequences; wherein each capture sequence binds specifically with one target nucleic acid fragment, and forms a double strand segment; washing out unbound target nucleic acid fragment; adding a first DNA probe, which has thereon a chemical label, to attach specifically to a probe binding site of the first target nucleic acid fragment; washing out unbound first probe; adding a triggering solution to trigger the chemical label; and detecting signals on the test snare for determining the first target nucleic acid fragment; subsequently, adding a second DNA probe to bind specifically to the second target nucleic acid fragment; washing, triggering and detecting signals for determining the second target nucleic acid fragment in the same manner.

Abstract translation: 用于顺序检测样品中多个靶核酸片段的方法包括以下步骤：将样品添加到具有多个单链捕获DNA序列的测试圈套的柱中; 其中每个捕获序列与一个靶核酸片段特异性结合，并形成双链链段; 洗去未结合的靶核酸片段; 加入其上具有化学标记的第一DNA探针，以特异性附着于第一靶核酸片段的探针结合位点; 洗出未结合的第一探针; 添加触发解决方案来触发化学标签; 以及检测所述测试圈套上的信号以确定所述第一靶核酸片段; 随后，加入第二DNA探针以特异性结合第二靶核酸片段; 以相同的方式洗涤，触发和检测用于确定第二靶核酸片段的信号。

公开(公告)号：US06470170B1

公开(公告)日：2002-10-22

申请号：US09858620

申请日：2001-05-16

Applicant: Hai Xing Chen ,  Qiang Li

Inventor： Hai Xing Chen ,  Qiang Li

IPC: G09B300

CPC classification number: G09B5/14 ,  G09B7/04

Abstract: A system for learning and examination training is disclosed. A student can log on the system through a website to review training history, to access a menu of levels of practice examinations of interest to him, and to select training exams on the website. During the examination, the student is able to indicate whether or not help is required at any given point or question. Without regard to whether or not the student believes help is required, all students are subject to a dynamic/interactive evaluation of responses in which a teacher who is on-line with the same website at the time of the student is able to intervene during an examination to invoke help options for the student. In the first instance, the teacher can intervene to change the exam level to either a lower or to a higher one. Among the help options available to a student during an examination within a given level are “auto coach” in which the student is provided with hints and examples; an adaptive question selection in which variations of the same questions are drawn from a database; real time talk support in which the on-line teacher can communicate with the student at the time that this option is invoked; and a multi-solution select option in which a database presents multiple means of problem to accommodate or discover the learning style of the student. The student can be scored, timed and graded in a variety of modes both during and after the examination.

Abstract translation: 披露了一个学习和考试培训系统。 学生可以通过网站登录系统，查看培训历史，访问他感兴趣的练习水平菜单，并在网站上选择培训考试。 在考试期间，学生能够表明在任何一点或问题上是否需要帮助。 不考虑学生是否需要帮助，所有学生都需要进行动态/互动的评估，在学生的同一网站在线同一网站的老师可以在 考试为学生提供帮助选择。 首先，老师可以进行干预，将考试级别更改为较低或更高级别。 在给定级别的考试期间，学生可以使用的帮助选项是“汽车教练”，其中提供了学生的提示和示例; 一种自适应问题选择，其中从数据库中提取相同问题的变体; 实时谈话支持，在线教师可以在调用此选项时与学生交流; 以及多解决方案选择选项，其中数据库提供多种问题的方式来适应或发现学生的学习风格。 在考试期间和考试之后，学生可以以各种模式得分，时间和等级。

公开(公告)号：US11344008B1

公开(公告)日：2022-05-31

申请号：US16295155

申请日：2019-03-07

Applicant: Hai Xing Chen ,  Hong Yang Wang ,  Zhongtian Qi

Inventor： Hai Xing Chen ,  Hong Yang Wang ,  Zhongtian Qi

IPC: A01K67/027 ,  C12N9/22 ,  C12N15/85

Abstract: A method of producing a non-erythroid protein in erythrocytes of a transgenic animal using an erythroid-specific promoter includes synthesizing an erythroid-specific globin gene promoter and globin gene locus control region and cloning the promoter and the globin gene locus control region and a gene encoding a non-erythroid protein into a vector to obtain a transgene; introducing the transgene in pronuclear embryos collected from a mammalian animal in vitro; transplanting the pronuclear embryos containing the transgene into oviduct of a female recipient of the mammalian animal to obtain a transgenic animal which then expresses the non-erythroid protein in progenitor cells of erythrocytes; and collecting blood from the transgenic animal and isolating the non-erythroid protein from the erythrocytes. Further disclosed are a transgenic animal expressing a non-erythroid protein in progenitor cells of erythrocytes and an isolated erythrocyte of a non-human transgenic animal containing a human non-erythroid protein encoded by a transgene.

公开(公告)号：US07794703B1

公开(公告)日：2010-09-14

申请号：US12634011

申请日：2009-12-09

Applicant: Hai Xing Chen

Inventor： Hai Xing Chen

IPC: A61K48/00 ,  A61K31/70 ,  A01N63/00 ,  C07H21/04 ,  C12N15/86

CPC classification number: A61K48/0075 ,  C12N2799/027 ,  C12N2830/008

Abstract: A method for production and delivery of a protein in vivo is described. The method includes steps of inserting a promoter and a gene encoding a protein in a vector, collecting an amount of host cells from a mammal, treating the host cells in vitro with the vector, then introducing the treated cells back to the mammal. In vivo, the treated host cells produce red blood cells and the protein which is contained in the red blood cells. The protein is released into peripheral blood of the mammal through a natural or an induced rupture of the red blood cells to supply the protein to the body.

Abstract translation: 描述了体内生产和递送蛋白质的方法。 该方法包括将启动子和编码蛋白质的基因插入载体中的步骤，从哺乳动物中收集一定量的宿主细胞，用载体在体外处理宿主细胞，然后将处理的细胞引入哺乳动物。 在体内，处理的宿主细胞产生红细胞和红细胞中所含的蛋白质。 蛋白质通过自然或诱导的红细胞破裂释放到哺乳动物的外周血中，以将蛋白质供应给身体。

公开(公告)号：US07482122B1

公开(公告)日：2009-01-27

申请号：US11255350

申请日：2005-10-19

Applicant: Hai Xing Chen

Inventor： Hai Xing Chen

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q2537/143 ,  C12Q2525/161

Abstract: A method of enhancing detection signal for measurement of nucleic acid sequences includes providing a T- or L-primary probe having a target-binding segment and an enhancer linker segment; and hybridizing the primary probe with a target sequence of interest; providing a first enhancement probe having a first annealing segment complementary to the enhancer linker segment, a second annealing segment and a chemical label; hybridizing the first enhancement probe to the primary probe; providing a second enhancement probe having a first annealing segment complementary to the second annealing segment of the first enhancement probe, a second annealing segment complementary to the first annealing segment of the first enhancement probe, and the same chemical label; hybridizing the first and second enhancement probes, thereby multiple first and second enhancement probes annealing with each other forming a staggered chain extending from the primary probe; and detecting the chemical labels in the final complex.

Abstract translation: 增强用于测量核酸序列的检测信号的方法包括提供具有靶结合片段和增强子连接子片段的T-或L-原代探针; 并将所述主要探针与感兴趣的目标序列杂交; 提供具有与增强子接头片段互补的第一退火部分的第一增强探针，第二退火部分和化学标签; 将第一增强探针与主探针杂交; 提供第二增强探针，其具有与第一增强探针的第二退火段互补的第一退火区段，与第一增强探针的第一退火段互补的第二退火区段和相同的化学标签; 使第一和第二增强探针杂交，由此多个第一和第二增强探针彼此退火形成从主探针延伸的交错链; 并检测最终复合物中的化学标记。

公开(公告)号：US09890418B1

公开(公告)日：2018-02-13

申请号：US15083086

申请日：2016-03-28

Applicant: Hai Xing Chen

Inventor： Hai Xing Chen

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q2537/143 ,  C12Q2563/107 ,  C12Q2563/149 ,  C12Q2565/519

Abstract: A method of enhancing signal in detection of nucleic acid sequences utilizes dual-functional capture particles for capturing a target sequence and for binding with enhancement probes coupled to a reporter. The method includes hybridizing a target sequence with a capture sequence on dual-functional capture particles; hybridizing a pair of enhancement probes to the bounded target sequence through a linker to form a staggered chain of enhancement probes; triggering a chemical reaction between a chemical binding group on the dual-functional capture particles and a first binding moiety on the enhancement probes to bind the enhancement probes covalently to dual-functional capture particles; binding a reporter to the bounded enhancement probes through another chemical reaction between a second binding moiety on the enhancement probes and a binding functional group on the reporter; and detecting the signal from a signal generating molecule on bounded reporter, thereby amplifying signal through enhancement probes coupled to reporters.

公开(公告)号：US07544474B2

公开(公告)日：2009-06-09

申请号：US11728238

申请日：2007-03-23

Applicant: Hai Xing Chen

Inventor： Hai Xing Chen

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12N5/0647 ,  A61K48/00 ,  A61K2035/124 ,  C12N5/0641 ,  C12N2510/02 ,  C12Q1/6837 ,  C12Q2565/102 ,  C12Q2565/519 ,  C12Q2537/125 ,  C12Q2537/149

Abstract: A method for sequentially detecting multiple target nucleic acid fragments in a sample includes steps of adding a sample into a column having a test snare which has thereon two or more single strand capture DNA sequences; wherein each capture sequence binds specifically with one target nucleic acid fragment, and forms a double strand segment; washing out unbound target nucleic acid fragment; adding a first DNA probe, which has thereon a chemical label, to attach specifically to a probe binding site of the first target nucleic acid fragment; washing out unbound first probe; adding a triggering solution to trigger the chemical label; and detecting signals on the test snare for determining the first target nucleic acid fragment; subsequently, adding a second DNA probe to bind specifically to the second target nucleic acid fragment; washing, triggering and detecting signals for determining the second target nucleic acid fragment in the same manner.

Abstract translation: 用于顺序检测样品中的多个靶核酸片段的方法包括将样品添加到具有测试圈套的柱中的步骤，所述测试圈套上具有两个或更多个单链捕获DNA序列; 其中每个捕获序列与一个靶核酸片段特异性结合，并形成双链链段; 洗去未结合的靶核酸片段; 加入其上具有化学标记的第一DNA探针，以特异性附着于第一靶核酸片段的探针结合位点; 洗出未结合的第一探针; 添加触发解决方案来触发化学标签; 以及检测所述测试圈套上的信号以确定所述第一靶核酸片段; 随后，加入第二DNA探针以特异性结合第二靶核酸片段; 以相同的方式洗涤，触发和检测用于确定第二靶核酸片段的信号。

公开(公告)号：US06337214B1

公开(公告)日：2002-01-08

申请号：US09326297

申请日：1999-06-04

Applicant: Hai Xing Chen

Inventor： Hai Xing Chen

IPC: G01N33566

CPC classification number: C12N5/0641 ,  A61K48/00 ,  A61K2035/124 ,  C12N5/0647 ,  C12N2510/02 ,  C12Q1/6806 ,  G01N33/54366 ,  G01N33/569 ,  C12Q2565/519

Abstract: Methods and apparatuses are disclosed for detecting the presence of a test material in a test sample. The test sample is introduced into a test column which has at least two snares. One of the snares has a control capture material for detection of the presence of control. Each of other snares has a capture material specific to a corresponding test material for which detection being sought. The capture material will bind with the corresponding test material to form a bound material. The test column is then washed to remove materials which have not been bound to the capture materials. Finally, the presence of bound materials is detected on each of the snares. The method is useful for detection of a pathogen indicator in a test sample, particularly suitable for detection of DNA and RNA.

Abstract translation: 公开了用于检测测试样品中测试材料的存在的方法和装置。 将测试样品引入具有至少两个圈套的测试柱中。 其中一个圈套具有用于检测控制存在的控制捕获材料。 每个其他圈套具有特定于相应测试材料的捕获材料，用于对其进行检测。 捕获材料将与相应的测试材料结合以形成结合材料。 然后洗涤测试柱以除去未与捕获材料结合的材料。 最后，在每个圈套上检测到结合物质的存在。 该方法可用于检测测试样品中的病原体指示剂，特别适用于检测DNA和RNA。