公开(公告)号：US06656734B1

公开(公告)日：2003-12-02

申请号：US09107271

申请日：1998-06-30

Applicant: Rainer Bischoff ,  Hanno Kolbe ,  Klaus Schughart

Inventor： Rainer Bischoff ,  Hanno Kolbe ,  Klaus Schughart

IPC: C12N1587

CPC classification number: C12N15/87

Abstract: The present invention relates to a composition which is intended for transferring at least one therapeutically active substance into the interior of a target cell, characterized in that it comprises a mixture of at least one therapeutically active substance and at least one polar compound which is selected from a specific group of aprotic polar compounds. The therapeutically active substance is preferably a polynucleotide, and the polar compound is DPSO, which improves the ability of the polynucleotide to be transfected into the interior of the cells. The compositions according to the invention can be used as a diagnostic therapeutic, prophylactic or vaccinal medicament for treating the human or animal body by means of gene therapy.

Abstract translation: 本发明涉及用于将至少一种治疗活性物质转移到靶细胞内部的组合物，其特征在于其包含至少一种治疗活性物质和至少一种极性化合物的混合物，所述极性化合物选自 一组非质子极性化合物。 治疗活性物质优选为多核苷酸，极性化合物为DPSO，其改善多核苷酸转染入细胞内部的能力。 根据本发明的组合物可以用作通过基因治疗来治疗人或动物体的诊断性治疗，预防或疫苗药物。

公开(公告)号：US20060223121A1

公开(公告)日：2006-10-05

申请号：US11450360

申请日：2006-06-12

Applicant: Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

Inventor： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

IPC: G01N33/53 ,  C07K14/74 ,  C07K16/28

CPC classification number: G01N33/564 ,  A61K38/1709 ,  C07K14/47 ,  G01N33/6896 ,  G01N2800/52

Abstract: The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide sequence in native state corresponds to SEQ ID No 1, SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, and SEQ ID No 29, and the peptide sequences having at least 70% identity, preferably at least 80% identity and advantageously at least 98% identity with any one of the peptide sequences SEQ ID No 1 to SEQ ID No 8 and SEQ ID No 10 to SEQ ID No 29, and the peptide sequences or fragments of said sequences belonging to a common family of proteins selected among perlecan, the precursor of the retinol-binding plasmatic protein, of the GM2 activator protein, of calgranulin B and of saponin B.

公开(公告)号：US07081345B1

公开(公告)日：2006-07-25

申请号：US10030937

申请日：2000-07-17

Applicant: Dominique Roecklin ,  Hanno Kolbe ,  Marie-Hélène Charles ,  Carine Malcus ,  Lyse Santoro ,  Hervé Perron

Inventor： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Hélène Charles ,  Carine Malcus ,  Lyse Santoro ,  Hervé Perron

IPC: G01N33/53

CPC classification number: G01N33/564 ,  A61K38/1709 ,  C07K14/47 ,  G01N33/6896 ,  G01N2800/52

Abstract: The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide sequence in native state corresponds to SEQ ID No 1, SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, and SEQ ID No 29, and the peptide sequences having at least 70% identity, preferably at least 80% identity and advantageously at least 98% identity with any one of the peptide sequences SEQ ID No 1 to SEQ ID No 8 and SEQ ID No 10 to SEQ ID No 29, and the peptide sequences or fragments of said sequences belonging to a common family of proteins selected among perlecan, the precursor of the retinol-binding plasmatic protein, of the GM2 activator protein, of calgranulin B and of saponin B.

公开(公告)号：US07510843B2

公开(公告)日：2009-03-31

申请号：US11450360

申请日：2006-06-12

Applicant: Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

Inventor： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

IPC: G01N33/53 ,  C07K16/18

CPC classification number: G01N33/564 ,  A61K38/1709 ,  C07K14/47 ,  G01N33/6896 ,  G01N2800/52

Abstract: The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide sequence in native state corresponds to SEQ ID No 1, SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, and SEQ ID No 29, and the peptide sequences having at least 70% identity, preferably at least 80% identity and advantageously at least 98% identity with any one of the peptide sequences SEQ ID No 1 to SEQ ID No 8 and SEQ ID No 10 to SEQ ID No 29, and the peptide sequences or fragments of said sequences belonging to a common family of proteins selected among perlecan, the precursor of the retinol-binding plasmatic protein, of the GM2 activator protein, of calgranulin B and of saponin B.

Abstract translation: 本发明涉及使用包含蛋白质片段的至少一种多肽以获得用于检测，预防或治疗与退行性和/或神经和/或自身免疫疾病相关的病理状况的诊断，预后，预防或治疗组合物，所述蛋白质 选自天然状态的肽序列的蛋白质对应于SEQ ID No 1，SEQ ID No 2，SEQ ID No 3，SEQ ID No 4，SEQ ID No 5，SEQ ID No 6，SEQ ID No 7，SEQ ID No 8，SEQ ID No 9，SEQ ID No 10，SEQ ID No 11，SEQ ID No 12，SEQ ID No 13，SEQ ID No 14，SEQ ID No 15，SEQ ID No 16，SEQ ID No 17，SEQ ID No 18，SEQ ID No 19，SEQ ID No 20，SEQ ID No 21，SEQ ID No 22，SEQ ID No 23，SEQ ID No 24，SEQ ID No 25，SEQ ID No 26，SEQ ID No 27，SEQ ID No 28和SEQ ID No 29，以及肽序列与肽序列SEQ ID No.1至SEQ ID NO：1至SEQ ID NO：1中的任何一个具有至少70％同一性，优选至少80％同一性，有利地至少98％的同一性 ID No 8和SEQ ID No 10至SEQ ID No 29，并且属于共同家族蛋白质的所述序列的肽序列或片段，其选自Perlecan，视黄醇结合性血浆蛋白的前体，GM2激活蛋白， 的钙粒蛋白B和皂角苷B.

公开(公告)号：US5276141A

公开(公告)日：1994-01-04

申请号：US835392

申请日：1992-02-14

Applicant: Hanno Kolbe

Inventor： Hanno Kolbe

IPC: C07K14/005 ,  C07K1/00 ,  C07K1/30 ,  C07K14/155 ,  C07K14/16 ,  C07K14/195 ,  C07K14/55 ,  C12P21/00 ,  C07K3/18 ,  C07K3/20 ,  C07K15/00 ,  C07K15/14

CPC classification number: C07K14/005 ,  C07K1/30 ,  C12N2740/16122 ,  Y10S435/814 ,  Y10S435/816

Abstract: The invention proposes a process for purifying a highly glycosylated protein from a crude preparation which comprises the action (i) of adding to said preparation a divalent metal ion in a sufficient amount in order to form a mixture which precipitates and (ii) after precipitation, of harvesting said protein from the mixture supernatant.

Abstract translation: 本发明提出了一种从粗制品中纯化高度糖基化的蛋白质的方法，其包括以下作用：（i）向所述制剂中加入足够量的二价金属离子以形成沉淀的混合物，和（ii）沉淀后， 从混合物上清液中收获所述蛋白质。