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14 条记录 (耗时 0.038 秒)

    METHODS OF MODIFYING LIGNIN BIOSYNTHESIS AND IMPROVING DIGESTIBILITY
    1.
    发明申请
    METHODS OF MODIFYING LIGNIN BIOSYNTHESIS AND IMPROVING DIGESTIBILITY 审中-公开
    修饰木质素生物合成和改善可溶性的方法

    公开(公告)号：US20120272406A1

    公开(公告)日：2012-10-25

    申请号：US13434035

    申请日：2012-03-29

    申请人： Hanping Guan ,  Beomseok Seo ,  Peifeng Ren ,  David Deppong ,  Huihua Fu

    发明人： Hanping Guan ,  Beomseok Seo ,  Peifeng Ren ,  David Deppong ,  Huihua Fu

    IPC分类号： C12N15/82 ,  C12N5/10 ,  C12N1/13 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N15/113 ,  A01H5/00

    CPC分类号： C12N9/1011 ,  C12N15/8255 ,  C12Y201/01104

    摘要： The present invention relates to methods for increasing digestibility and/or crude protein of a plant by modifying lignin biosynthesis of the plant. The methods involve the manipulation of the expression level of genes in the lignin biosynthesis pathway by directly reducing the expression of genes using miRNA or using regulation of transcription factors. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Transcription regulating nucleotide sequences for use in the expression cassettes have also been developed as well as methods of using these sequences and cassettes.

    摘要翻译： 本发明涉及通过改变植物的木质素生物合成来提高植物的消化率和/或粗蛋白质的方法。 该方法涉及通过使用miRNA直接降低基因的表达或使用转录因子的调节来操纵木质素生物合成途径中基因的表达水平。 还提供了用于实现这种基因表达操纵的表达盒和包含构建体和盒的转基因植物细胞和植物。 还开发了用于表达盒的转录调节核苷酸序列以及使用这些序列和盒的方法。

    Methods controlling gene expression
    7.
    发明授权
    Methods controlling gene expression 有权
    控制基因表达的方法

    公开(公告)号：US09085774B2

    公开(公告)日：2015-07-21

    申请号：US11918554

    申请日：2006-04-13

    申请人： Peifeng Ren ,  Hee-Sook Song ,  John McMillan

    发明人： Peifeng Ren ,  Hee-Sook Song ,  John McMillan

    IPC分类号： C12N15/82 ,  C12N15/79

    CPC分类号： C12N15/8218 ,  C12N15/79 ,  C12N15/8216

    摘要： The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. The present invention specifically provides sequences of naturally occurring, tissue-specifically expressed microRNAs. The invention further provides for transgenic expression constructs comprising sequences encoding said microRNAs. By incorporation of the microRNA encoding sequence the expression from said expression construct is specifically silenced in the tissue where the naturally occurring microRNA is naturally expressed. Thereby the expression profile resulting from the promoter is modulated and leakiness is reduced. The invention further provides for a method for modulating transgenic expression by incorporating sequences encoding said microRNAs into transgenic expression constructs. The compositions and methods of the invention can be used to enhance performance of agricultural relevant crops and for therapy, prophylaxis, research and diagnostics in diseases and disorders, which afflict mammalian species.

    摘要翻译： 本发明涉及遗传学，特别是植物遗传学领域，并提供能够控制基因表达的试剂。 本发明具体提供了天然存在的组织特异性表达的微小RNA的序列。 本发明还提供包含编码所述微小RNA的序列的转基因表达构建体。 通过并入microRNA编码序列，所述表达构建体的表达在天然存在的微RNA天然表达的组织中被特异性沉默。 因此，由启动子产生的表达谱被调节并且泄漏降低。 本发明还提供了通过将编码所述微小RNA的序列并入转基因表达构建体来调节转基因表达的方法。 本发明的组合物和方法可用于增强农业相关作物的性能以及影响哺乳动物物种的疾病和病症的治疗，预防，研究和诊断。

    RNA-Mediated Induction of Gene Expression in Plants
    8.
    发明申请
    RNA-Mediated Induction of Gene Expression in Plants 审中-公开
    RNA介导的植物基因表达诱导

    公开(公告)号：US20120036594A1

    公开(公告)日：2012-02-09

    申请号：US13264841

    申请日：2010-04-16

    申请人： Vinitha Joyce Cardoza ,  Peifeng Ren ,  Lawrence Winfield Talton

    发明人： Vinitha Joyce Cardoza ,  Peifeng Ren ,  Lawrence Winfield Talton

    IPC分类号： A01H1/00 ,  C07H21/02 ,  C12N5/10 ,  C12N1/00 ,  C12Q1/68 ,  C12N15/82

    CPC分类号： C12N15/8218 ,  C12N15/8293 ,  C12N15/8294

    摘要： The present invention is in the field of plant genetics and provides methods for increasing gene expression of a target gene in a plant or part thereof. In addition the invention relates to methods for modifying the specificity of plant specific promoters and for engineering small non-coding activating RNA (sncaRNA) in order to increase expression of a target gene in a plant or part thereof. The present invention also provides methods for the identification of sncaRNA, and its primary transcripts in a plant capable of increasing gene expression in a plant or part thereof.

    摘要翻译： 本发明涉及植物遗传学领域，提供了增加植物或其部分中靶基因基因表达的方法。 此外，本发明涉及用于修饰植物特异性启动子的特异性和用于工程化小型非编码活化RNA（sncaRNA）的方法，以增加植物或其部分中靶基因的表达。 本发明还提供了在能够增加植物或其部分中的基因表达的植物中鉴定sncaRNA及其初级转录物的方法。

    Improved Methods Controlling Gene Expression
    10.
    发明申请
    Improved Methods Controlling Gene Expression 有权
    改进控制基因表达的方法

    公开(公告)号：US20090293148A1

    公开(公告)日：2009-11-26

    申请号：US11918554

    申请日：2006-04-13

    申请人： Peifeng Ren ,  Hee-Sook Song ,  John McMillan

    发明人： Peifeng Ren ,  Hee-Sook Song ,  John McMillan

    IPC分类号： A01H1/00 ,  A01H5/10 ,  C07H21/04 ,  C12N15/63 ,  C12N5/04 ,  A01H5/00 ,  C12N5/06 ,  A01K67/027 ,  C12N1/21 ,  C12N1/15

    CPC分类号： C12N15/8218 ,  C12N15/79 ,  C12N15/8216

    摘要： The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. The present invention specifically provides sequences of naturally occurring, tissue-specifically expressed microRNAs. The invention further provides for transgenic expression constructs comprising sequences encoding said microRNAs. By incorporation of the microRNA encoding sequence the expression from said expression construct is specifically silenced in the tissue where the naturally occurring microRNA is naturally expressed. Thereby the expression profile resulting from the promoter is modulated and leakiness is reduced. The invention further provides for a method for modulating transgenic expression by incorporating sequences encoding said microRNAs into transgenic expression constructs. The compositions and methods of the invention can be used to enhance performance of agricultural relevant crops and for therapy, prophylaxis, research and diagnostics in diseases and disorders, which afflict mammalian species.

    摘要翻译： 本发明涉及遗传学，特别是植物遗传学领域，并提供能够控制基因表达的试剂。 本发明具体提供了天然存在的组织特异性表达的微小RNA的序列。 本发明还提供包含编码所述微小RNA的序列的转基因表达构建体。 通过并入microRNA编码序列，所述表达构建体的表达在天然存在的微RNA天然表达的组织中被特异性沉默。 因此，由启动子产生的表达谱被调节并且泄漏降低。 本发明还提供了通过将编码所述微小RNA的序列并入转基因表达构建体来调节转基因表达的方法。 本发明的组合物和方法可用于增强农业相关作物的性能以及影响哺乳动物物种的疾病和病症的治疗，预防，研究和诊断。