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公开(公告)号：US20110306094A1
公开(公告)日：2011-12-15
申请号：US13202446
申请日：2010-02-18
CPC分类号： C12P21/005 , C07K16/00 , C07K2317/14 , C07K2317/21 , C07K2317/55 , C12P19/34 , C12Q1/6804 , C12Q1/6858 , C12Q1/6881 , C12Q2600/16 , C12Q2549/119 , C12Q2537/143
摘要： The current invention is directed to a method for obtaining a nucleic acid encoding an immunoglobulin variable domain from a single cell comprising the following steps:—performing a first polymerase chain reaction with three to six 5′-primer and one 3′-primer, performing with the product of the first polymerase chain reaction a second polymerase chain reaction with thirteen to sixteen 5′-primer and one 3′-primer, whereby the distance of the binding locations of the primer employed in the second polymerase chain reaction is reduced compared to the first polymerase chain reaction.
摘要翻译： 本发明涉及从单细胞获得编码免疫球蛋白可变结构域的核酸的方法，包括以下步骤： - 用3至6个5'-引物和1个3'-引物进行第一聚合酶链反应，进行 与第一聚合酶链反应的产物进行第二聚合酶链反应与十三至十六个5'-引物和一个3'-引物，由此在第二聚合酶链式反应中使用的引物的结合位置的距离比 第一次聚合酶链反应。
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公开(公告)号：US09399670B2
公开(公告)日：2016-07-26
申请号：US13202446
申请日：2010-02-18
CPC分类号： C12P21/005 , C07K16/00 , C07K2317/14 , C07K2317/21 , C07K2317/55 , C12P19/34 , C12Q1/6804 , C12Q1/6858 , C12Q1/6881 , C12Q2600/16 , C12Q2549/119 , C12Q2537/143
摘要： The current invention is directed to a method for obtaining a nucleic acid encoding an immunoglobulin variable domain from a single cell comprising the following steps: —performing a first polymerase chain reaction with three to six 5′-primer and one 3′-primer, performing with the product of the first polymerase chain reaction a second polymerase chain reaction with thirteen to sixteen 5′-primer and one 3′-primer, whereby the distance of the binding locations of the primer employed in the second polymerase chain reaction is reduced compared to the first polymerase chain reaction.
摘要翻译： 本发明涉及从单细胞获得编码免疫球蛋白可变结构域的核酸的方法，包括以下步骤： - 用3至6个5'-引物和1个3'-引物进行第一聚合酶链反应，进行 与第一聚合酶链反应的产物进行第二聚合酶链反应与十三至十六个5'-引物和一个3'-引物，由此在第二聚合酶链式反应中使用的引物的结合位置的距离比 第一次聚合酶链反应。
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