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    Fluorescent double stranded DNA binding dyes
    1.
    发明授权
    Fluorescent double stranded DNA binding dyes 有权
    荧光双链DNA结合染料

    公开(公告)号：US08058431B2

    公开(公告)日：2011-11-15

    申请号：US12417735

    申请日：2009-04-03

    申请人： Hans-Peter Josel ,  Dieter Heindl ,  Bernhard Irlinger ,  Christian Weilke

    发明人： Hans-Peter Josel ,  Dieter Heindl ,  Bernhard Irlinger ,  Christian Weilke

    IPC分类号： C07D417/06

    CPC分类号： C09B23/04

    摘要： Disclosed is a fluorescent dye comprising a benzothiazolium moiety and a pyrimidinium moiety connected by a mono-methine bridge, characterized in that (i) the 2-position of the pyrimidine carries a substituent which starts with a C-atom and (ii) the 5- and 6- positions of the pyrimidine ring are an integral part of a further aromatic ring structure as exemplified by the following:

    摘要翻译： 公开了包含苯并噻唑鎓部分和通过单次甲基桥连接的嘧啶鎓部分的荧光染料，其特征在于（i）嘧啶的2-位携带以C原子开始的取代基，（ii）5 - 和6-位嘧啶环是进一步芳环结构的组成部分，如下列举例所示：

    Labeling reagent
    3.
    发明申请
    Labeling reagent 有权
    标记试剂

    公开(公告)号：US20070196852A1

    公开(公告)日：2007-08-23

    申请号：US11707399

    申请日：2007-02-16

    申请人： Dieter Heindl ,  Frank Bergmann ,  Hans-Peter Josel ,  Dirk Kessler ,  Gunter Lampert ,  Christian Weilke

    发明人： Dieter Heindl ,  Frank Bergmann ,  Hans-Peter Josel ,  Dirk Kessler ,  Gunter Lampert ,  Christian Weilke

    IPC分类号： C12Q1/68 ,  C07H19/04 ,  C07H21/04

    CPC分类号： C07H19/23 ,  C07H19/04 ,  C07H21/00 ,  C07H21/04 ,  Y02P20/55

    摘要： The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.

    摘要翻译： 本发明将取代的吲哚核苷作为两个末端以及用于核酸检测，分析和定量的标记的寡核苷酸探针的内部构建块重述。 取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。 这些修饰的核苷允许进入广泛的应用领域。 这些新的取代的吲哚核苷可以用作标记试剂，用于容易地制备例如 优化的杂交探针，Taqman探针或分子信标探针。

    Labeling reagent
    6.
    发明授权
    Labeling reagent 有权
    标记试剂

    公开(公告)号：US07759470B2

    公开(公告)日：2010-07-20

    申请号：US11707399

    申请日：2007-02-16

    申请人： Dieter Heindl ,  Frank Bergmann ,  Hans-Peter Josel ,  Dirk Kessler ,  Gunter Lampert ,  Christian Weilke

    发明人： Dieter Heindl ,  Frank Bergmann ,  Hans-Peter Josel ,  Dirk Kessler ,  Gunter Lampert ,  Christian Weilke

    IPC分类号： C07G3/00 ,  C07H21/02 ,  C07H21/04 ,  C07H19/04 ,  C12Q1/68

    CPC分类号： C07H19/23 ,  C07H19/04 ,  C07H21/00 ,  C07H21/04 ,  Y02P20/55

    摘要： The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.

    摘要翻译： 本发明将取代的吲哚核苷作为两个末端以及用于核酸检测，分析和定量的标记的寡核苷酸探针的内部构建块重述。 取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。 这些修饰的核苷允许进入广泛的应用领域。 这些新的取代的吲哚核苷可以用作标记试剂，用于容易地制备例如 优化的杂交探针，Taqman探针或分子信标探针。

    Reaction mixture for positioning a reaction vessel relative to a detection unit
    9.
    发明申请
    Reaction mixture for positioning a reaction vessel relative to a detection unit 审中-公开
    用于相对于检测单元定位反应容器的反应混合物

    公开(公告)号：US20060040303A1

    公开(公告)日：2006-02-23

    申请号：US11203845

    申请日：2005-08-15

    申请人： Christian Weilke

    发明人： Christian Weilke

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： G01N21/6428 ,  G01N21/0332 ,  G01N21/11 ,  G01N21/13 ,  G01N21/645 ,  G01N2021/0325 ,  G01N2021/6439 ,  G01N2201/08

    摘要： The present invention concerns methods for measuring a nucleic acid amplification in real-time comprising (i) providing a real-time PCR instrument containing a reaction vessel holder for holding multiple reaction vessels and means for positioning the multiple reaction vessels in a reaction vessel holder relative to the detection unit, (ii) filling multiple reaction vessels with a reaction mixture containing thermostable polymerase, deoxynucleotides and buffer, at least two amplification primers, at least one fluorescently-labelled hybridization probe and a fluorescent dye component which is present in a free form during and after the entire amplification reaction, (iii) determining the position of maximum fluorescence emission of the fluorescent dye component in multiple reaction vessels as a function of their position relative to the detection unit, and (iv) performing the amplification reaction and measuring the fluorescence emission of the at least one hybridization probe during and optionally after the amplification reaction for each reaction vessel at the position of maximum fluorescence emission.

    摘要翻译： 本发明涉及实时测量核酸扩增的方法，包括（i）提供包含用于保持多个反应容器的反应容器保持器的实时PCR仪器和用于将多个反应容器定位在反应容器保持器相对的装置 （ii）用含有热稳定聚合酶，脱氧核苷酸和缓冲液的反应混合物填充多个反应容器，至少两个扩增引物，至少一个荧光标记的杂交探针和以游离形式存在的荧光染料组分 在整个扩增反应期间和之后，（iii）确定多个反应容器中的荧光染料组分的最大荧光发射的位置，作为它们相对于检测单元的位置的函数，和（iv）进行扩增反应并测量 所述至少一个杂交探针在和期间的荧光发射 任选地在每个反应容器在最大荧光发射位置的扩增反应之后。