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    Specific binding assay using enzyme inhibitor and anti-inhibitor antibodies
    2.
    发明授权
    Specific binding assay using enzyme inhibitor and anti-inhibitor antibodies 失效
    使用酶抑制剂和抗抑制剂抗体的特异性结合测定

    公开(公告)号：US5916757A

    公开(公告)日：1999-06-29

    申请号：US683247

    申请日：1996-07-17

    申请人： Paul B. Contestable ,  John L. Daiss ,  Holly L. Groth ,  Elizabeth A. Grogan ,  Brian A. Snyder

    发明人： Paul B. Contestable ,  John L. Daiss ,  Holly L. Groth ,  Elizabeth A. Grogan ,  Brian A. Snyder

    IPC分类号： G01N33/58 ,  G01N33/53

    CPC分类号： G01N33/581

    摘要： Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, an immobilized reporter enzyme, an inhibitor antibody and a a water-soluble conjugate of the ligand and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme, but the antibodies affect enzymatic activity differently. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the generated signal to the target specific binding ligand.

    摘要翻译： 可以用利用配体的固定化受体，固定的报道酶，抑制剂抗体和配体的水溶性缀合物和抗抑制剂抗体的测定法来检测特异性结合配体。 两种抗体对于报道酶是特异的，但抗体对不同的酶活性影响。 当抑制剂抗体与其复合时，抑制剂抗体有效地抑制了报道酶的活性。 抗抑制剂抗体与报道酶结合，不影响酶活性，但不会阻止抑制酶的结合。 该测定提供所产生的信号与靶特异性结合配体的直接相关性。

    Test kit and method for competitive specific binding assay
    3.
    发明授权
    Test kit and method for competitive specific binding assay 失效
    用于竞争性特异性结合测定的测试试剂盒和方法

    公开(公告)号：US5589344A

    公开(公告)日：1996-12-31

    申请号：US259942

    申请日：1994-06-15

    申请人： Paul B. Contestable ,  Brian A. Snyder

    发明人： Paul B. Contestable ,  Brian A. Snyder

    IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/88

    CPC分类号： G01N33/88 ,  G01N33/54306

    摘要： One or more specific binding ligands can be detected with a competitive immunoassay which utilizes a water-soluble conjugate of ligand and reporter enzyme for each target ligand. Target ligand is allowed to compete for a first receptor with the conjugate. Uncomplexed conjugate is then contacted with an immobilized second receptor to form a reaction product which can be used to generate a detectable signal. This assay provides a direct correlation of the generated signal to the one or more target ligands.

    摘要翻译： 可以用竞争性免疫测定来检测一个或多个特异性结合配体，其利用配体和报道酶对于每个靶配体的水溶性缀合物。 允许靶配体与缀合物竞争第一受体。 然后将未络合的缀合物与固定化的第二受体接触以形成可用于产生可检测信号的反应产物。 该测定提供所产生的信号与一种或多种靶配体的直接相关性。

    Test kit and method for the detection of microorganisms associated with periodontal diseases using surfactant mixture as extraction composition
    4.
    发明授权
    Test kit and method for the detection of microorganisms associated with periodontal diseases using surfactant mixture as extraction composition 失效
    使用表面活性剂混合物作为提取组合物检测与牙周病相关的微生物的测试试剂盒和方法

    公开(公告)号：US5334503A

    公开(公告)日：1994-08-02

    申请号：US773833

    申请日：1991-10-08

    申请人： Brian A. Snyder ,  Paul B. Contestable ,  Catherine T. Abrams ,  Elizabeth A. Grogan

    发明人： Brian A. Snyder ,  Paul B. Contestable ,  Catherine T. Abrams ,  Elizabeth A. Grogan

    IPC分类号： G01N33/531 ,  G01N33/569

    CPC分类号： G01N33/56955 ,  G01N33/531 ,  Y10S435/961 ,  Y10S435/975

    摘要： An extraction composition is described which is buffered to a pH from about 8 to about 11 and which contains from about 1 to about 10 weight percent of a water-soluble cationic surfactant which is a quaternary ammonium salt or a mixture thereof and from about 1 to about 10 weight percent of an anionic surfactant which has a sulfate anion having from 6 to 14 carbon atoms and an alkali metal or ammonium cation. The extraction composition is used to extract antigens from Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis or Prevotella intermedia. Extracted antigens are determined using immunological methods. The extraction composition can be supplied as part of a diagnostic test kit.

    摘要翻译： 描述了提取组合物，其缓冲至约8至约11的pH，并且其含有约1至约10重量％的水溶性阳离子表面活性剂，其为季铵盐或其混合物，并且约1至约10重量％ 约10重量％的具有6-14个碳原子的硫酸根阴离子和碱金属或铵阳离子的阴离子表面活性剂。 提取组合物用于从放线杆菌放线菌，牙龈卟啉单胞菌或中间型Prevotella提取抗原。 使用免疫学方法测定提取的抗原。 提取组合物可以作为诊断测试试剂盒的一部分提供。

    Test kit and method for the determination of Streptococcus A antigen
    5.
    发明授权
    Test kit and method for the determination of Streptococcus A antigen 失效
    用于测定链球菌A抗原的测试试剂盒和方法

    公开(公告)号：US4808524A

    公开(公告)日：1989-02-28

    申请号：US131618

    申请日：1987-12-11

    申请人： Brian A. Snyder ,  Paul B. Contestable

    发明人： Brian A. Snyder ,  Paul B. Contestable

    IPC分类号： C12Q1/28 ,  G01N33/76 ,  C12Q1/14

    CPC分类号： G01N33/76 ,  C12Q1/28 ,  C12Q2326/00 ,  G01N2333/315 ,  Y10S435/81

    摘要： A test kit useful for the determination of Streptococcus A antigen comprises: (i) an immunoreactive reagent comprising either Streptococcus A antigen or antibodies attached to water-insoluble particles, (ii) a substrate having thereon a dried, binder-free coating of a first extraction reagent, (iii) an aqueous solution of a second extraction reagent, and (iv) a neutralizing solution. Both extraction reagents combine to provide nitrous acid. In addition, an extraction device includes a water-insoluble container having affixed therein the first extraction reagent and an applicator means for collecting and depositing a biological specimen within the container. The extraction device and test kit are useful to the determination of Streptococcus A antigen in a biological specimen.

    摘要翻译： 可用于测定链球菌A抗原的测试试剂盒包括：（i）包含链球菌A抗原或附着于水不溶性颗粒的抗体的免疫反应试剂，（ii）其上具有干燥的无粘合剂的第一 提取试剂，（iii）第二提取试剂的水溶液，和（iv）中和溶液。 两种提取试剂结合提供亚硝酸。 此外，提取装置包括其中固定有第一提取试剂的水不溶性容器和用于在生物体内收集和沉积生物样本的施用装置。 提取装置和测试试剂盒可用于测定生物样品中的链球菌A抗原。

    Use of blocking protein with high pH extraction in method to determine a microorganism associated with periodontal disease and kit useful therefor
    6.
    发明授权
    Use of blocking protein with high pH extraction in method to determine a microorganism associated with periodontal disease and kit useful therefor 失效
    在方法中使用具有高pH提取的阻断蛋白来测定与牙周病相关的微生物和用于其的试剂盒

    公开(公告)号：US5399484A

    公开(公告)日：1995-03-21

    申请号：US773064

    申请日：1991-10-08

    申请人： Bradley P. Boyer ,  Paul B. Contestable ,  Brian A. Snyder

    发明人： Bradley P. Boyer ,  Paul B. Contestable ,  Brian A. Snyder

    IPC分类号： G01N33/531 ,  G01N33/53 ,  G01N33/569

    CPC分类号： G01N33/56955 ,  G01N33/5306 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/975

    摘要： A method has been developed for determining microorganisms associated with periodontal diseases which is highly sensitive and shows very low background and cross-reactivity among various closely related antigens. Antigen is extracted at relatively high pH, and either before or immediately after extraction, the antigen-containing specimen is mixed with a blocking composition having at least about 0.2 weight percent of a non-immunoreactive blocking protein. The pH of the resulting mixture is kept high when contacted with the antibodies specific to the antigen of interest. The compositions and components needed for the assay can be supplied in a diagnostic test kit.

    摘要翻译： 已经开发了一种用于确定与牙周疾病相关的微生物的方法，所述微生物是高度敏感的并且在各种紧密相关的抗原之间显示非常低的背景和交叉反应性。 抗原在相对高的pH下提取，并且在提取之前或之后，将含抗原的样品与具有至少约0.2重量％的非免疫反应性阻断蛋白的封闭组合物混合。 当与感兴趣的抗原特异的抗体接触时，所得混合物的pH保持较高。 测定所需的组合物和组分可以在诊断测试试剂盒中提供。

    Wash composition for determination of microorganisms associated with periodontal diseases
    8.
    发明授权
    Wash composition for determination of microorganisms associated with periodontal diseases 失效
    洗涤组合物用于测定与牙周病相关的微生物

    公开(公告)号：US5378629A

    公开(公告)日：1995-01-03

    申请号：US72029

    申请日：1993-06-07

    申请人： Bradley P. Boyer ,  Paul B. Contestable ,  Brian A. Snyder

    发明人： Bradley P. Boyer ,  Paul B. Contestable ,  Brian A. Snyder

    IPC分类号： A61K8/00 ,  A61K8/46 ,  A61Q11/00 ,  C11D1/14 ,  C11D17/00 ,  G01N33/531 ,  G01N33/569

    CPC分类号： G01N33/56955 ,  G01N33/531 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S435/975 ,  Y10S436/826 ,  Y10T436/10 ,  Y10T436/107497

    摘要： An aqueous wash composition has been found useful in methods for determination of specific binding ligands. The composition is buffered to a pH of less than or equal to 6 or greater than or equal to 9. It also includes as its essential component at least about 0.1 weight percent of an anionic surfactant which is represented by the formula:[A-SO.sub.3+y ].sup.-(1+y) [X.sup.+m ].sub.nwherein A is a hydrocarbon having a molecular weight of at least about 180, X.sup.+m is hydrogen or a monovalent or divalent cation, m is 1 or 2, is 0 or 1, and n is 1 or 2 provided that m and n are not both 2. Optionally and preferably, the wash composition also includes a nonimmunoreactive protein. This wash composition is particularly useful in methods for determination of microorganisms associated with periodontal diseases. Such methods can be of a variety of formats, but immunometric assays are particularly useful. The wash composition can be included as part of a diagnostic test kit.

    摘要翻译： 已经发现水性洗涤组合物可用于测定特异性结合配体的方法。 将组合物缓冲至小于或等于6或大于或等于9的pH。还包括作为其主要成分的至少约0.1重量％的由下式表示的阴离子表面活性剂：[A-SO 3 + y] - （1 + y）[X + m] n其中A是分子量为至少约180的烃，X + m为氢或一价或二价阳离子，m为1或2，为0 或1，并且n为1或2，条件是m和n不同时为2。任选且优选地，洗涤组合物还包括非免疫反应性蛋白。 该洗涤组合物特别可用于测定与牙周病有关的微生物的方法。 这样的方法可以是多种形式，但是免疫测定法是特别有用的。 洗涤组合物可以作为诊断测试试剂盒的一部分包括在内。

    Diagnostic test kit and specific binding assay using modulator of signal resulting from peroxidase label
    10.
    发明授权
    Diagnostic test kit and specific binding assay using modulator of signal resulting from peroxidase label 失效
    诊断测试试剂盒和使用过氧化物酶标记产生的信号调节剂的特异性结合测定

    公开(公告)号：US5460946A

    公开(公告)日：1995-10-24

    申请号：US43246

    申请日：1993-04-06

    申请人： Paul B. Contestable ,  Bradley P. Boyer ,  Brian A. Snyder ,  Thomas R. Kissel

    发明人： Paul B. Contestable ,  Bradley P. Boyer ,  Brian A. Snyder ,  Thomas R. Kissel

    IPC分类号： C12Q1/28 ,  G01N33/531 ,  G01N33/545 ,  G01N33/58 ,  G01N33/569

    CPC分类号： G01N33/581 ,  C12Q1/28 ,  Y10S435/962

    摘要： The signal generated in a specific binding assay wherein a peroxidase label is used to detect the resulting specific binding complex on a microporous filtration membrane can be modulated by contacting the signal forming reagents with a buffered solution of a hydroxamic acid or acyl hydrazine having the structureR--CO--NH--R'or an equivalent salt thereof, wherein R is aryl of 6 to 10 carbon atoms in the aromatic nucleus, alkyl of 1 to 7 carbon atoms or cycloalkyl of 5 to 10 carbon atoms in the ring, and R' is hydroxy or amino. This solution can be provided in a diagnostic test kit for use in various methods to detect a specific binding ligand. The result is improved signal stability and lowered background after the use of a high pH wash solution in the assay.

    摘要翻译： 在特异性结合测定中产生的信号，其中过氧化物酶标记用于检测微孔过滤膜上产生的特异性结合复合物可以通过使信号形成试剂与具有结构R的异羟肟酸或酰基肼的缓冲溶液接触来调节 -CO-NH-R'或其等价盐，其中R是芳族核中6至10个碳原子的芳基，1至7个碳原子的烷基或环中5至10个碳原子的环烷基，R' 是羟基或氨基。 该解决方案可以在用于检测特异性结合配体的各种方法的诊断测试试剂盒中提供。 结果是在测定中使用高pH洗涤溶液后信号稳定性提高，背景降低。