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    Transgenic expression constructs for vegetative plant tissue specific expression of nucleic acids
    2.
    发明申请
    Transgenic expression constructs for vegetative plant tissue specific expression of nucleic acids 审中-公开
    用于营养植物组织特异性表达核酸的转基因表达构建体

    公开(公告)号：US20070174927A1

    公开(公告)日：2007-07-26

    申请号：US10591550

    申请日：2005-02-26

    申请人： Hee-Sook Song ,  Christina Roche ,  Marc Morra ,  Christian Dammann ,  Timothy Jensen ,  Alleson Dobson

    发明人： Hee-Sook Song ,  Christina Roche ,  Marc Morra ,  Christian Dammann ,  Timothy Jensen ,  Alleson Dobson

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8223

    摘要： The invention relates to transgenic expression constructs and vectors comprising plant promoters with a non-seed tissue, preferably vegetative plant tissue specific expression profile, and the use of these transgenic expression constructs or vectors for the transgenic expression of nucleic acid sequences in plants. More preferably, the promoters of the invention are the promoter of the Pisum sativum ptxA gene, or the promoter of the Glycine max extensin (SbHRGP3) gene, and functional equivalent fragments and functional equivalent homologs thereof, or their complements, having essentially the same promoter activity. The invention furthermore relates to transgenic plants and plant cells transformed with these expression constructs or vectors, to cultures, parts or propagation material derived therefrom, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

    摘要翻译： 本发明涉及包含具有非种子组织的植物启动子，优选营养植物组织特异性表达谱的转基因表达构建体和载体，以及这些转基因表达构建体或载体在植物中核酸序列的转基因表达的用途。 更优选地，本发明的启动子是豌豆豌豆ptxA基因的启动子，或甘氨酸最大延伸蛋白（SbHRGP3）基因的启动子及其功能等同片段及其功能等同同系物或其互补物，其基本上具有相同的启动子 活动。 本发明还涉及用这些表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料以及用于制备食品，动物饲料，种子，药物或精细化学品的转基因植物和植物细胞， 以改善植物生物量，产量或提供期望的表型。

    Expression enhancing intron sequences
    4.
    发明授权
    Expression enhancing intron sequences 失效
    表达增强内含子序列

    公开(公告)号：US08759506B2

    公开(公告)日：2014-06-24

    申请号：US13241493

    申请日：2011-09-23

    申请人： Hee-Sook Song ,  Christian Dammann ,  Marc Morra ,  Jeffrey A. Brown ,  Liqun Xing ,  Hongmei Jia

    发明人： Hee-Sook Song ,  Christian Dammann ,  Marc Morra ,  Jeffrey A. Brown ,  Liqun Xing ,  Hongmei Jia

    IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/113 ,  C12N15/63 ,  C12N15/82 ,  C12N1/21

    CPC分类号： C12N15/8241 ,  C12N15/8216 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8234

    摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

    摘要翻译： 本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。 本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。 本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。 本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或 精细化学品，以改善植物生物量，产量或提供期望的表型。

    Expression Enhancing Intron Sequences
    5.
    发明申请
    Expression Enhancing Intron Sequences 有权
    表达增强内含子序列

    公开(公告)号：US20090144863A1

    公开(公告)日：2009-06-04

    申请号：US11885988

    申请日：2006-03-07

    申请人： Hee-Sook Song ,  Christian Dammann ,  Marc Morra ,  Jeffrey A. Brown ,  Liqun Xing ,  Hongmei Jia

    发明人： Hee-Sook Song ,  Christian Dammann ,  Marc Morra ,  Jeffrey A. Brown ,  Liqun Xing ,  Hongmei Jia

    IPC分类号： A01H5/00 ,  C12Q1/68 ,  C40B30/00 ,  C12N15/00 ,  C12N15/11 ,  C12N5/04

    CPC分类号： C12N15/8241 ,  C12N15/8216 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8234

    摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

    摘要翻译： 本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。 本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。 本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。 本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或 精细化学品，以改善植物生物量，产量或提供期望的表型。

    EXPRESSION ENHANCING INTRON SEQUENCES
    6.
    发明申请
    EXPRESSION ENHANCING INTRON SEQUENCES 失效
    表达增强内容序列

    公开(公告)号：US20120054922A1

    公开(公告)日：2012-03-01

    申请号：US13241493

    申请日：2011-09-23

    申请人： Hee-Sook Song ,  Christian Dammann ,  Marc Morra ,  Jeffrey A. Brown ,  Liqun Xing ,  Hongmei Jia

    发明人： Hee-Sook Song ,  Christian Dammann ,  Marc Morra ,  Jeffrey A. Brown ,  Liqun Xing ,  Hongmei Jia

    IPC分类号： C40B30/04 ,  C12N1/21 ,  C40B30/02 ,  C12N1/19 ,  C12N5/10 ,  A01H5/00 ,  C12N15/63 ,  C12N1/15

    CPC分类号： C12N15/8241 ,  C12N15/8216 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8234

    摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

    摘要翻译： 本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。 本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。 本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。 本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或 精细化学品，以改善植物生物量，产量或提供期望的表型。

    Expression enhancing intron sequences
    7.
    发明授权
    Expression enhancing intron sequences 有权
    表达增强内含子序列

    公开(公告)号：US08088971B2

    公开(公告)日：2012-01-03

    申请号：US11885988

    申请日：2006-03-07

    申请人： Hee-Sook Song ,  Marc Morra ,  Liqun Xing ,  Hongmei Jia

    发明人： Hee-Sook Song ,  Marc Morra ,  Liqun Xing ,  Hongmei Jia

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8241 ,  C12N15/8216 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8234

    摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

    摘要翻译： 本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。 本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。 本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。 本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或 精细化学品，以改善植物生物量，产量或提供期望的表型。