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公开(公告)号：US09868956B2
公开(公告)日：2018-01-16
申请号：US13376068
申请日：2010-06-02
Applicant: Duy P. Nguyen , Heinz Neumann , Alexander Deiters , Jason Chin , Hrvoje Lusic
Inventor： Duy P. Nguyen , Heinz Neumann , Alexander Deiters , Jason Chin , Hrvoje Lusic
IPC: C12P21/06 , C12N15/67 , C07C233/49 , C07C271/22 , C12N9/00 , C12P21/02
CPC classification number: C12N15/67 , C07C233/49 , C07C271/22 , C12N9/93 , C12P21/02
Abstract: The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.
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公开(公告)号：US20120264926A1
公开(公告)日：2012-10-18
申请号：US13517372
申请日：2010-12-20
Applicant: Jason Chin , Kaihang Wang , Heinz Neumann
Inventor： Jason Chin , Kaihang Wang , Heinz Neumann
Abstract: The invention relates to 16S rRNA comprising a mutation at A1196, and to 16S rRNA further comprising a mutation at C1195 and/or A1197, and to 16S rRNA which comprises (i) C1195A and A1196G; or (ii) C1195T, A1196G and A1197G; or (iii) A1196G and A1197G. The invention also relates to ribosomes comprising such 16S rRNAs and to use of same.
Abstract translation: 本发明涉及包含A1196突变的16S rRNA和进一步包含C1195和/或A1197突变的16S rRNA和16S rRNA，其包含（i）C1195A和A1196G; 或（ii）C1195T，A1196G和A1197G; 或（iii）A1196G和A1197G。 本发明还涉及包含这种16S rRNA的核糖体并使用它们。
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公开(公告)号：US20110027829A1
公开(公告)日：2011-02-03
申请号：US12739247
申请日：2008-10-27
Applicant: Heinz Neumann , Jason Chin
Inventor： Heinz Neumann , Jason Chin
Abstract: The invention relates to a tRNA synthetase capable of binding N⊂-acetyl lysine, in particular, the invention relates to a tRNA synthetase capable of binding N∈-acetyl lysine wherein said synthetase comprises a polypeptide having at least 90% sequence identity to the amino acid sequence of MbPy1RS. The invention also relates to a method of making a polypeptide comprising N∈-acetyl lysine comprising arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, wherein said translation is carried out in the presence of a polypeptide according to any of claims 1 to 11 and in the presence of tRNA capable of being charged with N∈-acetyl lysine, and in the presence of N∈-acetyl lysine.
Abstract translation: 本发明涉及能够结合N-乙酰赖氨酸的tRNA合成酶，特别地，本发明涉及能够结合Nε-乙酰赖氨酸的tRNA合成酶，其中所述合成酶包含与氨基具有至少90％序列同一性的多肽 MbPy1RS的酸序列。 本发明还涉及一种制备包含Nε-乙酰赖氨酸的多肽的方法，包括安排编码所述多肽的RNA的翻译，其中所述RNA包含琥珀密码子，其中所述翻译在多肽存在下进行， 权利要求1至11中任一项，并且在存在能够被Nε-乙酰赖氨酸加载的tRNA存在下，并且在Nε-乙酰赖氨酸存在下。
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公开(公告)号：US20100105565A1
公开(公告)日：2010-04-29
申请号：US12516230
申请日：2007-11-28
Applicant: Jason Chin , Kaihang Wang , Heinz Neumann
Inventor： Jason Chin , Kaihang Wang , Heinz Neumann
CPC classification number: C12N15/11 , C12N15/111 , C12N2320/13 , C12P21/02 , C40B10/00 , C40B40/08
Abstract: There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.
Abstract translation: 提供了用于进化正交rRNA分子的方法，包括以下步骤：提供一个或多个突变正交rRNA分子文库，并将文库引入细胞，使得正交rRNA被并入核糖体中以提供正交核糖体; 提供（i）不被天然核糖体翻译的一个或多个正交mRNA分子，和（ii）包含一个或多个正交mRNA密码子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子，其中步骤（c）中的测定需要在正交mRNA中翻译一个或多个正交mRNA密码子; 并结合这样的rRNA分子的正交核糖体。
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公开(公告)号：US08497231B2
公开(公告)日：2013-07-30
申请号：US12516230
申请日：2007-11-28
Applicant: Jason Chin , Kaihang Wang , Heinz Neumann
Inventor： Jason Chin , Kaihang Wang , Heinz Neumann
CPC classification number: C12N15/11 , C12N15/111 , C12N2320/13 , C12P21/02 , C40B10/00 , C40B40/08
Abstract: There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.
Abstract translation: 提供了用于进化正交rRNA分子的方法，包括以下步骤：提供一个或多个突变正交rRNA分子文库，并将文库引入细胞，使得正交rRNA被并入核糖体中以提供正交核糖体; 提供（i）不被天然核糖体翻译的一个或多个正交mRNA分子，和（ii）包含一个或多个正交mRNA密码子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子，其中步骤（c）中的测定需要在正交mRNA中翻译一个或多个正交的mRNA密码子; 并结合这样的rRNA分子的正交核糖体。
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Patent Agency Ranking
公开(公告)号：US20120077948A1
公开(公告)日：2012-03-29
申请号：US13376068
申请日：2010-06-02
Applicant: Duy P. Nguyen , Heinz Neumann , Alexander Deiters , Jason Chin , Hrvoje Lusic
Inventor： Duy P. Nguyen , Heinz Neumann , Alexander Deiters , Jason Chin , Hrvoje Lusic
IPC: C12P21/00 , C07C233/49 , C07C271/22 , C08H1/00 , C07K14/00
CPC classification number: C12N15/67 , C07C233/49 , C07C271/22 , C12N9/93 , C12P21/02
Abstract: The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.
Abstract translation: 本发明涉及一种制备包含正交官能团的多肽的方法，所述正交官能团由脂肪族氨基酸或氨基酸衍生物构成，所述方法包括提供宿主细胞; 提供编码目的多肽的核酸; 提供与所述宿主细胞正交的tRNA-tRNA合成酶对; 加入包含所述正交官能团的氨基酸或氨基酸衍生物，其中所述氨基酸或氨基酸衍生物是所述正交tRNA合成酶的底物，其中所述氨基酸或氨基酸衍生物具有脂族碳骨架; 并孵育以允许所述氨基酸或氨基酸衍生物通过正交的tRNA-tRNA合成酶对引入目的多肽。 本发明还涉及某些氨基酸，以及包含其的多肽。
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公开(公告)号：US20120190825A1
公开(公告)日：2012-07-26
申请号：US13499340
申请日：2010-10-01
Applicant: Heinz Neumann , Jason Chin
Inventor： Heinz Neumann , Jason Chin
Abstract: The invention relates to a tRNA synthetase capable of binding Nε-acetyl lysine, wherein said synthetase comprises a polypeptide having at least 90% sequence identity to the amino acid sequence of MbPyIRS, and wherein said synthetase comprises a L266M mutation.
Abstract translation: 本发明涉及能够结合N-乙酰基赖氨酸的tRNA合成酶，其中所述合成酶包含与MbPyIRS的氨基酸序列具有至少90％序列同一性的多肽，并且其中所述合成酶包含L266M突变。
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公开(公告)号：US4910011A
公开(公告)日：1990-03-20
申请号：US284210
申请日：1988-12-14
Applicant: Karl-Heinz Dorr , Hugo Grimm , Heinz Neumann , Norbert Olhms
Inventor： Karl-Heinz Dorr , Hugo Grimm , Heinz Neumann , Norbert Olhms
CPC classification number: B01D53/8637
Abstract: In flue gases or other humid exhaust gases which are relatively cold and contain SO.sub.2, NO.sub.2 and other gaseous pollutants, the SO.sub.2 content is oxidized to SO.sub.3 and the NO content is reduced by a catalytic treatment, the SO.sub.3 content is condensed as sulfuric acid and other gaseous pollutants are removed by being scrubbed with aqueous liquids at low temperatures. In order to heat up the flue gas to the temperature required for the catalysis, the flue gas is heated up in a first heating-up stage by an indirect heat exchange with the catalytically treated gas before the catalytic treatment, the heated-up gas is heated up further in a second heating-up stage to the temperature required for the catalytic treatment, the catalytically treated gas which has been cooled in the first heating-up stage is cooled further in an indirect heat exchanger below the dew point temperature of sulfuric acid and is subsequently fed to an SO.sub.3 condenser. The remaining sulfuric acid vapor is absorbed by sprayed dilute sulfuric acid to form a dilute sulfuric acid having a predetermined concentration, the gas leaving the SO.sub.3 condenser is scrubbed in a scrubber with a sprayed aqueous liquid and is thus cooled to 40.degree. to 60.degree. C. and the purified gas is reheated to the required chimney inlet temperature.
Abstract translation: 在相对较冷且含有SO2，NO2和其他气态污染物的烟道气或其他潮湿废气中，SO2含量被氧化为SO3，通过催化处理使NO含量降低，SO3含量以硫酸等浓缩 通过在低温下用含水液体洗涤除去气态污染物。 为了将烟气加热至催化所需的温度，在催化处理之前，通过与催化处理气体的间接热交换，在第一加热阶段将烟道气加热，加热气体为 在第二加热阶段进一步加热到催化处理所需的温度，在第一加热阶段中被冷却的催化处理气体在低于硫酸露点温度的间接热交换器中进一步冷却 并随后进料至SO 3冷凝器。 剩余的硫酸蒸气被喷射的稀硫酸吸收，形成具有预定浓度的稀硫酸，离开SO 3冷凝器的气体在洗涤器中用喷射的水性液体洗涤，并被冷却至40℃至60℃ 并将净化的气体再加热至所需的烟囱入口温度。
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公开(公告)号：US07981260B2
公开(公告)日：2011-07-19
申请号：US11575219
申请日：2005-09-12
Applicant: Guenther Huber , Reinhard Oettl , Manfred Munzinger , Heinz Neumann , Martina Schulz , Matthias Stahl , Gerhard Ruf , Ralph Hamleser , Hans-Juergen Bender , Volker Esswein
Inventor： Guenther Huber , Reinhard Oettl , Manfred Munzinger , Heinz Neumann , Martina Schulz , Matthias Stahl , Gerhard Ruf , Ralph Hamleser , Hans-Juergen Bender , Volker Esswein
Abstract: An electrolysis cell for preparing alkali metals from a liquid alkali metal heavy metal alloy, including a tube arranged essentially horizontally having a closure device at each of the two ends of the tube. At least one solid electrolyte tube arranged concentrically in the tube and oriented with openings towards one end of the tube such that a first annular gap for conducting a liquid alkali metal, which forms an anode, is present between the inside of the tube and the outside of the solid electrolyte tube. An interior space in the solid electrolyte tube, sealed off from an alloy inlet, first annular gap and an alloy outlet, accommodates liquid alkali metal that can be used as a cathode.
Abstract translation: 一种用于从液态碱金属重金属合金制备碱金属的电解槽，包括基本上水平布置的管，在管的两端各有一个闭合装置。 至少一个固体电解质管同心地设置在管中并且朝向管的一端定向，使得在管的内部和外部之间存在用于导入形成阳极的液态碱金属的第一环形间隙 的固体电解质管。 在固体电解质管中，从合金入口，第一环形间隙和合金出口密封的内部空间容纳可用作阴极的液态碱金属。
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公开(公告)号：US4842835A
公开(公告)日：1989-06-27
申请号：US74645
申请日：1987-07-17
Applicant: Karl H. Dorr , Hugo Grimm , Heinz Neumann , Wolfgang Fennemann , Norbert Ohlms
Inventor： Karl H. Dorr , Hugo Grimm , Heinz Neumann , Wolfgang Fennemann , Norbert Ohlms
CPC classification number: B01D53/8637 , C01B17/74
Abstract: Flue gases or other contaminated humid exhaust gases which contain SO.sub.3 are cooled by an indirect heat exchange in a heat exchanger to a temperature below the dew point temperature of sulfuric acid and are cooled further to a predetermined temperature in an SO.sub.3 condenser by means of dilute sulfuric acid, which is sprayed to flow in a countercurrent to the gas, whereby dilute sulfuric acid having a predetermined concentration is condensed. The gas leaving the SO.sub.3 condenser is scrubbed and cooled to a temperature from 40.degree. to 55.degree. C. in a fine scrubber by a treatment with a sprayed aqueous liquor. The exit gas is reheated to a predetermined temperature in a reheater by a treatment with sprayed dilute sulfuric acid from the SO.sub.3 and after the condensation of SO.sub.3 is passed through a filter. Dilute sulfuric acid from the reheater is sprayed into the SO.sub.3 condenser. The temperature of the gas in the reheater is so selected that the water which has been absorbed is not condensed but remains in the gaseous effluent. The exit temperature of the gas leaving the heat exchanger is approximately so selected that the temperature at which dilute sulfuric acid becomes available is approximately the same as the temperature of the gas in the reheater. The rate at which dilute sulfuric acid is sprayed is so adjusted that the dilute sulfuric acid withdrawn from the fine scrubber is at a temperature which is approximately the same as the required exit temperature of the gas leaving the SO.sub.3 condenser.
Abstract translation: 含有SO 3的烟气或其它受污染的潮湿废气通过在热交换器中的间接热交换冷却到低于硫酸露点温度的温度，并通过稀硫酸在SO 3冷凝器中进一步冷却至预定温度 酸，其被喷射以与气体逆流流动，由此浓缩具有预定浓度的稀硫酸。 离开SO 3冷凝器的气体通过用喷射的含水液体处理在细洗涤器中被洗涤并冷却至40℃至55℃的温度。 通过用SO 3的喷射稀硫酸处理并且在SO 3的冷凝通过过滤器之后，将出口气体在再热器中再加热至预定温度。 将稀硫酸从再热器喷入SO 3冷凝器。 再热器中的气体的温度如此选择，使得被吸收的水不会冷凝，而是保留在气态流出物中。 离开热交换器的气体的出口温度大致如此选择，使得稀硫酸可用的温度与再热器中的气体的温度大致相同。 调节稀硫酸的速率被调整为使得从精细洗涤器中排出的稀硫酸处于与离开SO 3冷凝器的气体的出口温度大致相同的温度。
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