摘要:
The rotating union for two different fluids comprises a hollow shaft (2) mounted rotatably in a stationary housing (1), with two separate axial throughflow channels (4, 5). A slide ring packing assembly is provided between the hollow shaft (2) and two separate inlet channels (9, 17) provided in the housing (1) for the two fluids. This includes a common slide ring (8) which is connected at its face to the hollow shaft (2) and comprises a central bore (8a) and several axial passages (8b) arranged concentrically thereto, and in the housing (1) a central slide ring (11) and a slide ring (14) concentrically surrounding the latter. The concentric slide ring (14) comprises several axial passages (14a) which are arranged in a circle concentric to the axis of rotation (A) and connected to the inlet channel (17) for the second fluid. Between the outer circumference of the central slide ring (11) and the inner circumference of the concentric slide ring (14) is provided an annular chamber (18) extending as far as the sliding surface (8c) of the common slide ring (8) and communicating with an inlet channel (23) provided in the housing and an outlet channel (24).
摘要:
This invention relates to a method of detection of an mRNA having HPV and cellular sequences in a body sample, comprising the following steps:(a) obtaining a body sample;(b) isolating mRNA from the body sample of (a);(c) translating the mRNA of (b) into cDNA using a primer common for reverse transcription;(d) amplifying the cDNA of (c) by a PCR reaction with a 5' HPV primer and a 3' primer having sequences of the primer of (c);(e) cleaving the amplified cDNA of (d) with an endonuclease cleaving on the 5' side of the HPV polyadenylation sequence;(f) amplifying the non-cleaved cDNA of (e) with the primers of (d) or with "nested" primers; and(g) detecting the amplified cDNA of (f).Furthermore, this invention concerns the use of such a method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV, respectively.