Rotating union for two different fluids
    1.
    发明授权
    Rotating union for two different fluids 失效
    两种不同流体的旋转接头

    公开(公告)号:US5022686A

    公开(公告)日:1991-06-11

    申请号:US430737

    申请日:1989-11-02

    IPC分类号: B23Q11/10 F16L39/00

    摘要: The rotating union for two different fluids comprises a hollow shaft (2) mounted rotatably in a stationary housing (1), with two separate axial throughflow channels (4, 5). A slide ring packing assembly is provided between the hollow shaft (2) and two separate inlet channels (9, 17) provided in the housing (1) for the two fluids. This includes a common slide ring (8) which is connected at its face to the hollow shaft (2) and comprises a central bore (8a) and several axial passages (8b) arranged concentrically thereto, and in the housing (1) a central slide ring (11) and a slide ring (14) concentrically surrounding the latter. The concentric slide ring (14) comprises several axial passages (14a) which are arranged in a circle concentric to the axis of rotation (A) and connected to the inlet channel (17) for the second fluid. Between the outer circumference of the central slide ring (11) and the inner circumference of the concentric slide ring (14) is provided an annular chamber (18) extending as far as the sliding surface (8c) of the common slide ring (8) and communicating with an inlet channel (23) provided in the housing and an outlet channel (24).

    摘要翻译: 用于两种不同流体的旋转联接器包括可旋转地安装在固定壳体(1)中的中空轴(2),具有两个分离的轴向通流通道(4,5)。 滑动环填料组件设置在中空轴(2)和设置在两个流体的壳体(1)中的两个独立的入口通道(9,17)之间。 这包括一个共同的滑动环(8),它在其表面连接到中空轴(2)并且包括一个中心孔(8a)和与其同心设置的几个轴向通道(8b),并且在壳体(1) 滑动环(11)和同心地围绕其的滑动环(14)。 同心滑动环(14)包括多个轴向通道(14a),其被布置成与旋转轴线(A)同心的圆并连接到用于第二流体的入口通道(17)。 在中心滑环(11)的外圆周和同心滑环(14)的内圆周之间设置有延伸至公共滑环(8)的滑动表面(8c)的环形室(18) 并与设置在壳体中的入口通道(23)和出口通道(24)连通。

    Method of early detection of HPV-associated carcinomas and extreme
dysplasias caused by HPV
    2.
    发明授权
    Method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV 失效
    HPV相关癌的早期检测方法和HPV导致的极度异常发育不良

    公开(公告)号:US6027891A

    公开(公告)日:2000-02-22

    申请号:US913547

    申请日:1998-01-28

    IPC分类号: C12N15/09 C12Q1/68 C12Q1/70

    CPC分类号: C12Q1/686 C12Q1/708

    摘要: This invention relates to a method of detection of an mRNA having HPV and cellular sequences in a body sample, comprising the following steps:(a) obtaining a body sample;(b) isolating mRNA from the body sample of (a);(c) translating the mRNA of (b) into cDNA using a primer common for reverse transcription;(d) amplifying the cDNA of (c) by a PCR reaction with a 5' HPV primer and a 3' primer having sequences of the primer of (c);(e) cleaving the amplified cDNA of (d) with an endonuclease cleaving on the 5' side of the HPV polyadenylation sequence;(f) amplifying the non-cleaved cDNA of (e) with the primers of (d) or with "nested" primers; and(g) detecting the amplified cDNA of (f).Furthermore, this invention concerns the use of such a method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV, respectively.

    摘要翻译: PCT No.PCT / DE96 / 00306 Sec。 371日期1998年1月28日 102(e)日期1998年1月28日PCT提交1996年2月23日PCT公布。 WO96 / 26293 PCT出版物 日期:1996年8月29日本发明涉及检测身体样品中具有HPV和细胞序列的mRNA的方法,包括以下步骤:(a)获得身体样品; (b)从(a)的身体样品中分离mRNA; (c)使用通用于逆转录的引物将(b)的mRNA翻译成cDNA; (d)用5'HPV引物和具有(c)引物序列的3'引物通过PCR反应扩增(c)的cDNA; (e)用HPV聚腺苷酸化序列的5'侧切割内切酶切割(d)扩增的cDNA; (f)用(d)或“嵌套”引物的引物扩增(e)的未裂解的cDNA; 和(g)检测(f)的扩增cDNA。 此外,本发明涉及分别由HPV引起的早期检测HPV相关癌和极度发育不良的方法的使用。