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6 条记录 (耗时 0.015 秒)

    Process for the determination of antithrombin-BM
    1.
    发明授权
    Process for the determination of antithrombin-BM 失效
    测定抗凝血酶BM的方法

    公开(公告)号：US4496653A

    公开(公告)日：1985-01-29

    申请号：US394998

    申请日：1982-06-09

    申请人： Helmut Lill ,  Jurgen Schrenk ,  Peter Wunderwald

    发明人： Helmut Lill ,  Jurgen Schrenk ,  Peter Wunderwald

    IPC分类号： C12Q1/56 ,  C12Q1/38

    CPC分类号： C12Q1/56 ,  G01N2333/81 ,  G01N2333/8128 ,  G01N2333/974 ,  G01N2400/40

    摘要： For the determination of antithrombin-BM an antithrombin-BM-cofactor, thrombin and a determinable thrombin substrate are added to a sample solution and the splitting of the thrombin substrate is determined as a measure for the antithrombin-BM activity, whereby heparin, xylan, .lambda.-carrageenan, dextran sulphate, mucopolysaccharide polysulphuric acid esters, pentosan polysulphates or short-chained heparin derivatives produced by the chemical or enzymatic splitting of heparin are used as cofactors.

    摘要翻译： PCT No.PCT / DE81 / 00170 Sec。 371日期1982年6月9日 102（e）1982年6月9日PCT PCT 1981年10月8日PCT公布。 第WO82 / 01377号公报 日期为1982年4月29日。为了测定抗凝血酶-MB，将抗凝血酶-BM辅助因子，凝血酶和可测定的凝血酶底物加入到样品溶液中，凝血酶底物的分离被确定为抗凝血酶-BM 活性，其中肝素，木聚糖，λ-角叉菜胶，硫酸葡聚糖，粘多糖多硫酸酯，戊聚糖多硫酸酯或通过肝素的化学或酶切裂产生的短链肝素衍生物用作辅因子。

    Process for the selective separation of endoproteases
    2.
    发明授权
    Process for the selective separation of endoproteases 失效
    内切蛋白酶选择性分离的方法

    公开(公告)号：US4393139A

    公开(公告)日：1983-07-12

    申请号：US297467

    申请日：1981-08-28

    申请人： Jurgen Schrenk ,  Peter Wunderwald

    发明人： Jurgen Schrenk ,  Peter Wunderwald

    IPC分类号： C12N9/00 ,  C12N9/50 ,  C12N9/48 ,  C12N9/60

    CPC分类号： C12N9/50 ,  C12N9/00 ,  Y10S435/814

    摘要： The present invention provides a process for the selective separation of endoproteases from aqueous solutions, wherein an aqueous solution containing proteases is treated with a complex, present in the solid phase, of alpha.sub.2 -macroglobulin with a divalent metal selected from zinc, cobalt, nickel and copper and the solid phase then separated off.The present invention also provides an agent for carrying out this process, wherein it comprises a solid carrier material which is loaded with a complex of alpha.sub.2 -macroglobulin and of a divalent metal selected from zinc, cobalt, nickel and copper.

    摘要翻译： 本发明提供了一种从水溶液中选择性分离内切蛋白酶的方法，其中含有蛋白酶的水溶液用α2-巨球蛋白与固相中的复合物一起用选自锌，钴，镍的二价金属和 铜和固相然后分离。 本发明还提供了用于进行该方法的试剂，其中它包含固体载体材料，其载有α2-巨球蛋白复合物和选自锌，钴，镍和铜的二价金属。

    Endoproteinase-Lys-C and process for its preparation thereof
    3.
    发明授权
    Endoproteinase-Lys-C and process for its preparation thereof 失效
    内蛋白酶-Lys-C及其制备方法

    公开(公告)号：US4414332A

    公开(公告)日：1983-11-08

    申请号：US297480

    申请日：1981-08-28

    申请人： Jurgen Schrenk ,  Peter Wunderwald

    发明人： Jurgen Schrenk ,  Peter Wunderwald

    IPC分类号： C12N9/52 ,  C12Q1/37 ,  C12N9/50 ,  C12R1/01

    CPC分类号： C12N9/52 ,  Y10S435/822

    摘要： The present invention provides endoproteinase-Lys-C from bacteria, consisting of a chain of molecular weight 35000 to 38000 Dalton, having a pH optimum at pH 7.7 and being inhibited by aprotinin but not being inhibited by alpha.sub.2 -macroglobulin, .alpha..sub.1 -antitrypsin and ethylenediamine-tetraacetic acid.The present invention also provides a process for obtaining this enzyme, wherein a culture broth of an appropriate bacterial strain which has been filtered or pre-purified by usual methods is chromatographed over carrier-fixed alpha.sub.2 -macroglobulin-metal complex and the enzyme is obtained from the filtrate.

    摘要翻译： 本发明提供来自细菌的内蛋白酶-Lys-C，其由分子量为35000至38000道尔顿的链组成，其pH在pH7.7最适pH并被抑肽酶抑制，但不被α2-巨球蛋白，α1-抗胰蛋白酶抑制，和 乙二胺四乙酸。 本发明还提供了一种获得该酶的方法，其中通过常规方法过滤或预纯化的合适的细菌菌株的培养液在载体固定的α2-巨球蛋白 - 金属络合物上进行色谱分离， 滤液。

    Process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy
    6.
    发明授权
    Process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy 失效
    用于检测过敏的存在和对过敏原的特异性检测的过程

    公开(公告)号：US4592997A

    公开(公告)日：1986-06-03

    申请号：US476452

    申请日：1983-03-17

    申请人： Otto-Henning Wilhelms ,  Peter Stahl ,  Peter Wunderwald

    发明人： Otto-Henning Wilhelms ,  Peter Stahl ,  Peter Wunderwald

    IPC分类号： G01N33/53 ,  A61K39/00 ,  C12N9/64 ,  C12Q1/37 ,  C12Q1/38

    CPC分类号： C12N9/6421 ,  C12Q1/37 ,  C12Q2337/12

    摘要： The present invention provides a process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy, based on the cellular principle, wherein leukocytes of a sample to be investigated are incubated with an allergen or another stimulation factor of degranulation, such as anti-IgE, in an aqueous medium, the leukocytes are then separated off and the remaining solution is incubated with a compound of the general formula:Y--X--Cin which Y is an amino acid or a peptide containing 2 or 3 amino acids, the amino acids optionally carrying a conventional protective group on the amino end, X is arginine or lysine and C is a chromogenic residue, in the presence of a buffer effective at pH 6.3 to 8.0 and the liberation of the chromogenic residue is measured.

    摘要翻译： 本发明提供了一种检测过敏现象的检测方法和用于特异性检测过敏原的特异性检测方法，该方法基于细胞原理，其中待研究样品的白细胞与过敏原或另一种刺激物 脱水因子如抗IgE，然后将白细胞分离出来，剩下的溶液与下列通式的化合物一起温育：YXC，其中Y是氨基酸或含有2或3的肽 氨基酸，氨基酸任选地在氨基末端携带常规保护基，X是精氨酸或赖氨酸，C是发色残基，在pH6.3至8.0有效的缓冲液存在下，测定显色残留物的释放 。