公开(公告)号：US06987211B1

公开(公告)日：2006-01-17

申请号：US09310638

申请日：1999-05-12

申请人： Hermona Soreq ,  Haim Zakut ,  Moshe Shani

发明人： Hermona Soreq ,  Haim Zakut ,  Moshe Shani

IPC分类号： A01K67/027 ,  C12P21/00

CPC分类号： C12N15/8509 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2227/50 ,  A01K2267/03 ,  A01K2267/0356 ,  C12N9/18 ,  C12Q1/46

摘要： The present invention relates to novel alternative forms of human acetylcholinesterase (AChE) and nucleotide sequences encoding the same. The genes encoding the novel forms of human AChE have been identified in various malignant tumor cells. In a further aspect, the invention relates to a transgenic animal assay system for evaluating efficacy of drugs against cholinergic proteins, prior to or in the course of therapeutic treatment. Transgenic animals, preferably developing tadpole of Xenopus or mice which express human AChE, are used. The transgenic animal assay system is also useful for evaluating the toxicity of substances which potentially block human AChE (e.g. organophosphorous compounds).

摘要翻译： 本发明涉及人乙酰胆碱酯酶（AChE）的新型替代形式及其编码的核苷酸序列。 已经在各种恶性肿瘤细胞中鉴定了编码人类AChE的新型形式的基因。 在另一方面，本发明涉及用于在治疗治疗之前或治疗过程中评价药物对胆碱能蛋白的功效的转基因动物测定系统。 使用转基因动物，优选发育表达人AChE的非洲爪蟾的t us或小鼠。 转基因动物测定系统也可用于评估可能阻断人类AChE（例如有机磷化合物）的物质的毒性。

公开(公告)号：US6025183A

公开(公告)日：2000-02-15

申请号：US814095

申请日：1997-03-06

申请人： Hermona Soreq ,  Haim Zakut ,  Moshe Shani

发明人： Hermona Soreq ,  Haim Zakut ,  Moshe Shani

IPC分类号： A01K67/027 ,  C12N1/21 ,  C12N9/18 ,  C12N15/85

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  C12N9/18 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/50 ,  A01K2267/03 ,  A01K2267/0356

摘要： The present invention provides a transgenic animal assay system which provides a model system for testing for, and treatment of, cholinergic deficits and imbalances in mammals such as cognitive functioning in Alzheimer's patients, certain types of retinal photoreceptor degeneration, hematopoietic disorders, and screening for and susceptibility to anti-cholinesterase compounds. The transgenic animals and progeny thereof are transformed with a recombinant expression vector of the present invention. The recombinant expression vector comprises a DNA sequence encoding a heterologous cholinesterase (ChE) enzyme and promoter.

摘要翻译： 本发明提供了一种转基因动物测定系统，其提供用于测试和治疗哺乳动物胆碱能缺陷和不平衡的模型系统，例如阿尔茨海默病患者的认知功能，某些类型的视网膜光感受器变性，造血障碍，以及筛选和 对抗胆碱酯酶化合物的敏感性。 用本发明的重组表达载体转化转基因动物及其后代。 重组表达载体包含编码异源胆碱酯酶（ChE）酶和启动子的DNA序列。

公开(公告)号：US5932780A

公开(公告)日：1999-08-03

申请号：US370156

申请日：1995-01-09

申请人： Hermona Soreq ,  Haim Zakut ,  Moshe Shani

发明人： Hermona Soreq ,  Haim Zakut ,  Moshe Shani

IPC分类号： C12N9/18 ,  C12N15/85 ,  C12Q1/46 ,  A01K67/00 ,  A01K67/027 ,  A01K67/033 ,  G01N33/00

CPC分类号： C12N15/8509 ,  A01K67/0278 ,  C12N9/18 ,  C12Q1/46 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2227/50 ,  A01K2267/03 ,  A01K2267/0356

摘要： The present invention relates to novel alternative forms of human acetylcholinesterase (AChE) and nucleotide sequences encoding the same. The genes encoding the novel forms of human AChE have been identified in various malignant tumor cells. In a further aspect, the invention relates to a transgenic animal assay system for evaluating efficacy of drugs against cholinergic proteins, prior to or in the course of therapeutic treatment. Transgenic animals, preferably developing tadpole of Xenopus or mice which express human AChE, are used. The transgenic animal assay system is also useful for evaluating the toxicity of substances which potentially block human AChE (e.g. organophosphorous compounds).

摘要翻译： 本发明涉及人乙酰胆碱酯酶（AChE）的新型替代形式及其编码的核苷酸序列。 已经在各种恶性肿瘤细胞中鉴定了编码人类AChE的新型形式的基因。 在另一方面，本发明涉及用于在治疗治疗之前或治疗过程中评价药物对胆碱能蛋白的功效的转基因动物测定系统。 使用转基因动物，优选发育表达人AChE的非洲爪蟾的t us或小鼠。 转基因动物测定系统也可用于评估可能阻断人类AChE（例如有机磷化合物）的物质的毒性。

公开(公告)号：US5648243A

公开(公告)日：1997-07-15

申请号：US310356

申请日：1994-09-21

申请人： David R. Hurwitz ,  Margret Nathan ,  Moshe Shani

发明人： David R. Hurwitz ,  Margret Nathan ,  Moshe Shani

IPC分类号： A01K67/027 ,  C07K14/47 ,  C07K14/765 ,  C12N15/09 ,  C12N15/14 ,  C12N15/85

CPC分类号： C12N15/85 ,  A01K67/0278 ,  C07K14/4717 ,  C07K14/765 ,  C12N15/8509 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/102 ,  A01K2227/105 ,  A01K2267/01 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/85

摘要： The present invention provides DNA constructs comprising a promoter DNA sequence and a DNA sequence coding for human serum albumin. In one embodiment the human serum albumin sequence comprises at least one, but not all, of the introns in the naturally occurring gene encoding for the HSA protein. In another embodiment the DNA constructs comprise a 5' regulatory sequence which directs the expression and secretion of HSA protein in the milk of a transgenic animal. Preferably, the promoter gene is a milk protein promoter sequence such as .beta.-lactoglobulin. The present invention also provides transgenic animals which secrete HSA in the milk of lactating females. The present invention also provides vectors comprising the constructs of the present invention.

摘要翻译： 本发明提供了包含启动子DNA序列和编码人血清白蛋白的DNA序列的DNA构建体。 在一个实施方案中，人血清白蛋白序列包含编​​码HSA蛋白的天然存在的基因中的至少一个但不是全部的内含子。 在另一个实施方案中，DNA构建体包含引导转基因动物的乳中HSA蛋白的表达和分泌的5'调控序列。 优选地，启动子基因是乳蛋白启动子序列，例如β-乳球蛋白。 本发明还提供了在哺乳期雌性乳中分泌HSA的转基因动物。 本发明还提供了包含本发明构建体的载体。

公开(公告)号：US07803919B2

公开(公告)日：2010-09-28

申请号：US11427230

申请日：2006-06-28

申请人： Eyal Seroussi ,  Harris A. Lewin ,  Mark R. Band ,  Miri Cohen-Zinder ,  James K. Drackley ,  Denis M. Larkin ,  Juan J. Loor ,  Micha Ron ,  Moshe Shani ,  Joel Ira Weller

发明人： Eyal Seroussi ,  Harris A. Lewin ,  Mark R. Band ,  Miri Cohen-Zinder ,  James K. Drackley ,  Denis M. Larkin ,  Juan J. Loor ,  Micha Ron ,  Moshe Shani ,  Joel Ira Weller

IPC分类号： C07H21/04 ,  C07H21/02

CPC分类号： C12Q1/6888 ,  C07K14/705 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172

摘要： A quantitative trait locus (QTL) affecting milk fat and protein concentration was localized to a 4cM confidence interval on chromosome 6 centered on the microsatellite BM143. The genes and sequence variation in this region were characterized, and common haplotypes spanning five polymorphic sites in the genes IBSP, SPP1, PKD2, and ABCG2 for two sires heterozygous for this QTL were localized. Expression of SPP1 and ABCG2 in the bovine mammary gland increased from parturition through lactation. SPP1 was sequenced, and all the coding exons of ABCG2 and PKD2 were sequenced for these two sires. The single nucleotide change capable of encoding a substitution of tyrosine-581 to serine (Y581S) in the ABCG2 transporter was the only polymorphism corresponding to the segregation status of all three heterozygous and 15 homozygous sires for the QTL in the Israeli and US Holstein populations.

摘要翻译： 影响乳脂肪和蛋白质浓度的数量性状基因座（QTL）定位于以微卫星BM143为中心的染色体6上的4cM置信区间。 对该区域的基因和序列变异进行了表征，对于该QTL杂合的两个引物，IBSP，SPP1，PKD2和ABCG2基因的五个多态性位点的常见单倍型进行了定位。 牛乳腺中SPP1和ABCG2的表达从分娩到哺乳期增加。 对SPP1进行测序，并对这两个家系对ABCG2和PKD2的所有编码外显子进行测序。 在ABCG2转运蛋白中能够编码酪氨酸581替代丝氨酸（Y581S）的单核苷酸变化是唯一对应于以色列和美国荷斯坦人群中QTL所有三个杂合子和15个纯合子的分离状态的多态性。

公开(公告)号：US20080060271A1

公开(公告)日：2008-03-13

申请号：US10594564

申请日：2005-03-28

申请人： Ofer Beniamin ,  Dvir Arkin ,  Elyakim Reshef ,  Moshe Shani ,  Mordehay Yardeny ,  Avraham Atiias ,  Moshe Israel Granoi ,  Joseph Cohen ,  David Ben-Harosh

发明人： Ofer Beniamin ,  Dvir Arkin ,  Elyakim Reshef ,  Moshe Shani ,  Mordehay Yardeny ,  Avraham Atiias ,  Moshe Israel Granoi ,  Joseph Cohen ,  David Ben-Harosh

IPC分类号： E01F13/00

CPC分类号： E01F13/12

摘要： Devices for arresting a wheeled vehicle with rubber tires, comprising elongated flexible member (11) such as strips, mats, cables or chains disposed along the direction of motion and adapted to wrap around the tires using a tire attaching means (14) such as barb, spike or adhesive, the tire attaching means being fixed to the proximal end of the flexible member with respect to the vehicle's motion, and an arresting means (16) such as a rod attached to the distal end of the flexible member and adapted to interfere with the vehicle motion. The spikes may be anchoring in the tire and the cable or chain may be strong enough to tear the tires. The devices may be disposed in a flat housing with movable cover for allowing free passage of the vehicle.

摘要翻译： 用于用橡胶轮胎阻止轮式车辆的装置，包括细长的柔性构件（11），例如沿着运动方向设置的条，垫，电缆或链条，并且适于使用诸如倒钩的轮胎附接装置（14）包裹轮胎 所述轮胎安装装置相对于所述车辆的运动被固定到所述柔性构件的近端;以及止动装置（16），例如连接到所述柔性构件的远端的杆，并适于干涉 与车辆运动。 尖钉可以锚定在轮胎中，并且电缆或链条可能足够坚固以撕裂轮胎。 这些装置可以设置在具有可移动盖的平坦壳体中，以允许车辆自由通过。

公开(公告)号：US20080198060A1

公开(公告)日：2008-08-21

申请号：US11774832

申请日：2007-07-09

申请人： Moshe Shani ,  Avraham Cohen ,  Arye Amarant ,  Ely Levine

发明人： Moshe Shani ,  Avraham Cohen ,  Arye Amarant ,  Ely Levine

IPC分类号： G01S7/42

CPC分类号： G01S7/021 ,  F41H11/02 ,  F41J2/00 ,  G01S7/38 ,  H01Q1/28 ,  H01Q1/286 ,  H01Q9/16 ,  H01Q9/30 ,  H01Q11/08 ,  H01Q21/28

摘要： An expendable, stand-alone, off-board Electronic Counter-Measure system, airborne RF decoy aimed to provide airborne platforms with protection against multiple radar-based threats including Air-to-Air and Surface-to-Air missiles both active and semi-active ones. The airborne RF decoy has the mechanical outline of standard chaff and flare decoys and is safely ejected from any platform by pyrotechnic elements.The airborne RF decoy deceives enemy radar-based threats as follows: immediately after its ejection from the protected airborne platform, the decoy activates an energy source, stabilizes its path, acquires illuminating signals and analyzes threat parameters. Then the decoy alters the received signals to generate an authentic false target and transmits a deceiving signal towards the radar threat. The radar threats locks on the decoy and follow its path. Thus the threat course is diverted from the protected airborne platform and a large miss distance of the attacking missile (tens to hundreds of meters) is assured.

摘要翻译： 一个消耗性，独立的离线电子对抗系统，机载射频诱饵旨在提供机载平台，防止多种雷达威胁，包括空对空和地对空导弹，主动和半空， 活跃的。 机载RF诱饵具有标准谷壳和火炬诱饵的机械轮廓，并通过烟火元素从任何平台安全地弹出。 机载射频诱饵欺骗敌方基于雷达的威胁如下：从受保护的机载平台弹出后，诱饵激活能源，稳定其路径，获取照明信号并分析威胁参数。 然后，诱饵改变接收的信号以产生真实的假目标，并向雷达威胁发送欺骗信号。 雷达威胁锁在诱饵上并遵循其路径。 因此，威胁过程从受保护的机载平台转移，确保了攻击导弹（几十到几百米）的大错过距离。

公开(公告)号：US08049656B2

公开(公告)日：2011-11-01

申请号：US11774832

申请日：2007-07-09

申请人： Moshe Shani ,  Avraham Cohen ,  Arye Amarant ,  Ely Levine

发明人： Moshe Shani ,  Avraham Cohen ,  Arye Amarant ,  Ely Levine

IPC分类号： G01S7/38 ,  H04K3/00

CPC分类号： G01S7/021 ,  F41H11/02 ,  F41J2/00 ,  G01S7/38 ,  H01Q1/28 ,  H01Q1/286 ,  H01Q9/16 ,  H01Q9/30 ,  H01Q11/08 ,  H01Q21/28

摘要： An expendable, stand-alone, off-board Electronic Counter-Measure system, airborne RF decoy aimed to provide airborne platforms with protection against multiple radar-based threats including Air-to-Air and Surface-to-Air missiles both active and semi-active ones. The airborne RF decoy has the mechanical outline of standard chaff and flare decoys and is safely ejected from any platform by pyrotechnic elements. The airborne RF decoy deceives enemy radar-based threats as follows: immediately after its ejection from the protected airborne platform, the decoy activates an energy source, stabilizes its path, acquires illuminating signals and analyzes threat parameters. Then the decoy alters the received signals to generate an authentic false target and transmits a deceiving signal towards the radar threat. The radar threats locks on the decoy and follow its path. Thus the threat course is diverted from the protected airborne platform and a large miss distance of the attacking missile (tens to hundreds of meters) is assured.

摘要翻译： 一个消耗性，独立的离线电子对抗系统，机载射频诱饵旨在提供机载平台，防止多种雷达威胁，包括空对空和地对空导弹，主动和半空， 活跃的。 机载RF诱饵具有标准谷壳和火炬诱饵的机械轮廓，并通过烟火元素从任何平台安全地弹出。 机载射频诱饵欺骗敌方基于雷达的威胁如下：从受保护的机载平台弹出后，诱饵激活能源，稳定其路径，获取照明信号并分析威胁参数。 然后，诱饵改变接收的信号以产生真实的假目标，并向雷达威胁发送欺骗信号。 雷达威胁锁在诱饵上并遵循其路径。 因此，威胁过程从受保护的机载平台转移，确保了攻击导弹（几十到几百米）的大错过距离。

公开(公告)号：US20070118912A1

公开(公告)日：2007-05-24

申请号：US11427230

申请日：2006-06-28

申请人： Eyal Seroussi ,  Harris Lewin ,  Mark Band ,  Miri Cohen-Zinder ,  James Drackley ,  Denis Larkin ,  Juan Loor ,  Micha Ron ,  Moshe Shani ,  Joel Weller

发明人： Eyal Seroussi ,  Harris Lewin ,  Mark Band ,  Miri Cohen-Zinder ,  James Drackley ,  Denis Larkin ,  Juan Loor ,  Micha Ron ,  Moshe Shani ,  Joel Weller

IPC分类号： A01K67/027 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6888 ,  C07K14/705 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172

摘要： A quantitative trait locus (QTL) affecting milk fat and protein concentration was localized to a 4cM confidence interval on chromosome 6 centered on the microsatellite BM143. The genes and sequence variation in this region were characterized, and common haplotypes spanning five polymorphic sites in the genes IBSP, SPP1, PKD2, and ABCG2 for two sires heterozygous for this QTL were localized. Expression of SPP1 and ABCG2 in the bovine mammary gland increased from parturition through lactation. SPP1 was sequenced, and all the coding exons of ABCG2 and PKD2 were sequenced for these two sires. The single nucleotide change capable of encoding a substitution of tyrosine-581 to serine (Y581S) in the ABCG2 transporter was the only polymorphism corresponding to the segregation status of all three heterozygous and 15 homozygous sires for the QTL in the Israeli and US Holstein populations.

摘要翻译： 影响乳脂肪和蛋白质浓度的数量性状基因座（QTL）定位于以微卫星BM143为中心的染色体6上的4cM置信区间。 对该区域的基因和序列变异进行了表征，对于该QTL杂合的两个引物，IBSP，SPP1，PKD2和ABCG2基因的五个多态性位点的常见单倍型进行了定位。 牛乳腺中SPP1和ABCG2的表达从分娩到哺乳期增加。 对SPP1进行测序，并对这两个家系对ABCG2和PKD2的所有编码外显子进行测序。 在ABCG2转运蛋白中能够编码酪氨酸581替代丝氨酸（Y581S）的单核苷酸变化是唯一对应于以色列和美国荷斯坦人群中QTL所有三个杂合子和15个纯合子的分离状态的多态性。