公开(公告)号：US20230241609A1

公开(公告)日：2023-08-03

申请号：US18010794

申请日：2020-06-17

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Jeffrey A. Nielsen ,  Viktor Shkolnikov ,  Erica Squires ,  Roberto A. Pugliese ,  Matthew D. Smith ,  Michael J. Day ,  Diane R. Hammerstad

IPC: B01L3/00 ,  G01N15/10

CPC classification number: B01L3/502746 ,  G01N15/1031 ,  G01N15/1056 ,  B01L2300/0663 ,  B01L2400/08 ,  B01L2200/061 ,  B01L2200/027 ,  B01L2300/0867 ,  B01L2200/0647

Abstract: A method of detecting passage of a particle into a target location includes receiving a sample on a die including a microfluidic chamber, the microfluidic chamber including a microfluidic path coupling a reservoir to a foyer, and moving the sample from the reservoir to the foyer by firing a nozzle fluidically coupled to the foyer. The method further includes detecting passage of a particle of the sample from the reservoir to the foyer via a first sensor disposed within the microfluidic path, and detecting passage of the particle into the target location via a second sensor disposed between the first sensor and the nozzle. The method includes recording in a dispense map, an indication of whether the target location includes a single particle or multiple particles based on signals measured by the first sensor and the second sensor.