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12 条记录 (耗时 0.026 秒)

    Process for preparing purified active monomer of bone-derived factor
    1.
    发明授权
    Process for preparing purified active monomer of bone-derived factor 失效
    骨源性因子纯化活性单体的制备方法

    公开(公告)号：US07811793B2

    公开(公告)日：2010-10-12

    申请号：US10734583

    申请日：2003-12-15

    申请人： Hidetoshi Andou ,  Jun Honda ,  Shunjiro Sugimoto ,  Gertrud Hötten ,  Rolf Bechtold ,  Jens Pohl

    发明人： Hidetoshi Andou ,  Jun Honda ,  Shunjiro Sugimoto ,  Gertrud Hötten ,  Rolf Bechtold ,  Jens Pohl

    IPC分类号： C12P21/04 ,  C12P21/06 ,  C12N1/20 ,  C07K14/51

    CPC分类号： C07K14/51 ,  A61K38/00 ,  C07K14/475 ,  C07K14/575

    摘要： A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence: a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer, b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor, c) subjecting the refolded monomeric bone morphogenetic factor to purification.

    摘要翻译： 一种制备骨源性因子的纯化的重折叠单体或二聚体的方法，其包括使基因工程产生的骨衍生因子的内含体按顺序步骤a）至c）：a）将多核苷酸 将骨形态发生因子编码成细菌，以包涵体的形式表达所述骨形态发生因子，回收所述包涵体并用变性剂处理以获得溶解的单体，b）直接用 重折叠溶液以获得重折叠的单体骨形态发生因子，c）使重折叠的单体骨形态发生因子进行纯化。

    Use of MP52 or MP121 for treating and preventing diseases of the nervous system
    3.
    发明授权
    Use of MP52 or MP121 for treating and preventing diseases of the nervous system 失效
    使用MP52或MP121治疗和预防神经系统疾病

    公开(公告)号：US06531450B2

    公开(公告)日：2003-03-11

    申请号：US08981490

    申请日：1998-05-18

    申请人： Gertrud Hötten ,  Jens Pohl ,  Rolf Bechtold ,  Michael Paulista ,  Klaus Unsicker

    发明人： Gertrud Hötten ,  Jens Pohl ,  Rolf Bechtold ,  Michael Paulista ,  Klaus Unsicker

    IPC分类号： A61R3818

    CPC分类号： C07K14/475 ,  A61K38/1841 ,  C07K2319/00 ,  A61K2300/00

    摘要： The present invention concerns the use of biologically active MP52 or/and MP121 for the treatment and prevention of diseases of the nervous system or/and for the treatment of neuropathological situations which are caused by ageing of the nervous system. A pharmaceutical agent according to the invention for the treatment and prevention of diseases of the nervous system or/and for treating neuropathological situations which are caused by ageing of the nervous system therefore contains biologically active MP52 or/and MP121 as the active substance.

    摘要翻译： 本发明涉及生物活性MP52或/和MP121用于治疗和预防神经系统疾病和/或用于治疗由神经系统衰老引起的神经病理学情况的用途。 因此，根据本发明的用于治疗和预防由神经系统衰老引起的神经系统疾病和/或用于治疗神经病理学情况的本发明的药剂包含生物活性MP52或/和MP121作为活性物质。

    DNA sequences encoding growth/differentiation
    6.
    发明授权
    DNA sequences encoding growth/differentiation 失效
    编码生长/分化因子的DNA序列

    公开(公告)号：US06197550B1

    公开(公告)日：2001-03-06

    申请号：US09054526

    申请日：1998-04-03

    申请人： Gertrud Hötten ,  Helge Neidhardt ,  Rolf Bechtold ,  Jens Pohl

    发明人： Gertrud Hötten ,  Helge Neidhardt ,  Rolf Bechtold ,  Jens Pohl

    IPC分类号： C12N1519

    CPC分类号： C07K14/51 ,  A61K38/00 ,  C07K14/495

    摘要： The invention provides DNA sequences encoding novel members of the TGF-&bgr; family of proteins. The TGF-&bgr; family comprises proteins which function as growth and/or differentiation factors and which are useful in medical applications. Accordingly, the invention also describes the isolation of the above-mentioned DNA sequences, the expression of the encoded proteins, the production of said proteins and pharmaceutical compositions containing said proteins.

    摘要翻译： 本发明提供编码TGF-β蛋白家族的新成员的DNA序列。 TGF-β家族包含用作生长和/或分化因子并且可用于医学应用的蛋白质。 因此，本发明还描述了上述DNA序列的分离，编码蛋白质的表达，所述蛋白质的产生和含有所述蛋白质的药物组合物。

    Monomeric protein of the TGF-β family
    9.
    发明授权
    Monomeric protein of the TGF-β family 有权
    TGF-β家族的单体蛋白

    公开(公告)号：US07569227B2

    公开(公告)日：2009-08-04

    申请号：US11191072

    申请日：2005-07-28

    申请人： Gertrud Hötten ,  Rolf Bechtold ,  Jens Pohl

    发明人： Gertrud Hötten ,  Rolf Bechtold ,  Jens Pohl

    IPC分类号： A61K39/00 ,  A61K38/00 ,  C07K14/00 ,  A01N1/00

    CPC分类号： C07K14/495 ,  A61K38/00 ,  A61L27/227 ,  C07K14/475 ,  C07K14/575

    摘要： The present invention is concerned with proteins selected from the members of the TGF-β superfamily, which are monomeric due to substitution or deletion of a cysteine which is responsible for dimer formation. The invention is also concerned with nucleic acids, encoding such monomeric proteins, vectors or host cells containing the nucleic acids as well as with pharmaceutical compositions comprising the proteins or nucleic acids encoding the proteins. The compositions can be applied advantageously for all indications for which the respective dimeric proteins are useful.

    摘要翻译： 本发明涉及选自TGF-β超家族成员的蛋白质，其由于负责二聚体形成的半胱氨酸的取代或缺失而是单体的。 本发明还涉及编码这样的单体蛋白质的核酸，含有核酸的载体或宿主细胞以及包含编码蛋白质的蛋白质或核酸的药物组合物。 组合物可以有利地用于各种二聚体蛋白质可用的所有适应症。

    Growth/differentiation factor of the TGF-β family
    10.
    发明授权
    Growth/differentiation factor of the TGF-β family 失效
    TGF-β家族的生长/分化因子

    公开(公告)号：US07141239B2

    公开(公告)日：2006-11-28

    申请号：US11105344

    申请日：2005-04-14

    申请人： Gertrud Hötten ,  Helge Neidhardt ,  Rolf Bechtold ,  Jens Pohl

    发明人： Gertrud Hötten ,  Helge Neidhardt ,  Rolf Bechtold ,  Jens Pohl

    IPC分类号： C07K14/475 ,  A61K38/19

    CPC分类号： A61K38/1858 ,  A61K38/00 ,  A61K38/1808 ,  C07K14/495 ,  C07K14/51

    摘要： The invention provides DNA sequences encoding novel members of the TGF-β family of proteins. The TGF-β family comprises proteins which function as growth and/or differentiation factors and which are useful in medical applications. Accordingly, the invention also describes the isolation of the above-mentioned DNA sequences, the expression of the encoded proteins, the production of said proteins and pharmaceutical compositions containing said proteins.

    摘要翻译： 本发明提供编码TGF-β蛋白家族的新成员的DNA序列。 TGF-β家族包含用作生长和/或分化因子并且可用于医学应用的蛋白质。 因此，本发明还描述了上述DNA序列的分离，编码蛋白质的表达，所述蛋白质的产生和含有所述蛋白质的药物组合物。